A5072525407- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Enzyme Structure and Function
- Advanced Electron Microscopy Techniques and Applications
- Phytoplasmas and Hemiptera pathogens
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Magnetic properties of thin films
- Plant Pathogenic Bacteria Studies
- Carbohydrate Chemistry and Synthesis
- Plant-based Medicinal Research
- RNA Research and Splicing
- Plant biochemistry and biosynthesis
- Bacterial biofilms and quorum sensing
- CRISPR and Genetic Engineering
- Microbial Metabolism and Applications
- Genetics and Neurodevelopmental Disorders
- Galectins and Cancer Biology
- Protein Structure and Dynamics
- Plant Parasitism and Resistance
- Machine Learning in Bioinformatics
- Click Chemistry and Applications
- Lipid Membrane Structure and Behavior
ETH Zurich
2020-2023
National Institute of Chemistry
2017-2021
An extra sugar protects Many microbial pathogens produce proteins that are toxic to the cells they targeting. Broad-leaved plants susceptible NLP (necrosis and ethylene-inducing peptide 1–like protein) toxins. Lenarčič et al. identified receptors for toxins be GIPC (glycosylinositol phosphorylceramide) sphingolipids (see Perspective by Van den Ackerveken). Their findings reveal why these only attack broad-leaved (so-called eudicots): If sphingolipid carries just two hexoses, as is case...
The genetic code that specifies the identity of amino acids incorporated into proteins during protein synthesis is almost universally conserved. Mitochondrial genomes feature deviations from standard code, including reassignment two arginine codons to stop codons. required for translation termination at these noncanonical release newly synthesized polypeptides not currently known. In this study, we used gene editing and ribosomal profiling in combination with cryo-electron microscopy...
Mitochondrial ribosomes are specialized for the synthesis of membrane proteins responsible oxidative phosphorylation. Mammalian mitoribosomes have diverged considerably from ancestral bacterial and feature dramatically reduced ribosomal RNAs. The structural basis mammalian mitochondrial ribosome assembly is currently not well understood. Here we present eight distinct intermediates human large mitoribosomal subunit involving seven factors. We discover that NSUN4-MTERF4 dimer plays a critical...
Mitochondrial ribosomes (mitoribosomes) play a central role in synthesizing mitochondrial inner membrane proteins responsible for oxidative phosphorylation. Although mitoribosomes from different organisms exhibit considerable structural variations, recent insights into mitoribosome assembly suggest that maturation follows common principles and involves number of conserved factors. To investigate the steps involved mitoribosomal small subunit (mt-SSU) we determined cryoelectron microscopy...
Necrosis and ethylene-inducing peptide 1 (Nep1)-like proteins (NLPs) are secreted by several phytopathogenic microorganisms. They trigger necrosis in various eudicot plants upon binding to plant sphingolipid glycosylinositol phosphorylceramides (GIPC). Interestingly, HaNLP3 from the obligate biotroph oomycete Hyaloperonospora arabidopsidis does not induce necrosis. We determined crystal structure of showed that it adopts NLP fold. However, conformations loops surrounding GIPC...
The lack of efficient methods to control the major diseases crops most important agriculture leads huge economic losses and seriously threatens global food security. Many microbial plant pathogens, including bacteria, fungi, oomycetes, secrete necrosis- ethylene-inducing peptide 1 (Nep1)-like proteins (NLPs), which critically contribute virulence spread disease. NLPs are cytotoxic eudicot plants, as they disturb plasma membrane by binding specific sphingolipid receptors. Their pivotal role...
Aegerolysins are proteins produced by bacteria, fungi, plants and protozoa. The most studied fungal aegerolysins share a common property of interacting with membranes enriched cholesterol in combination either sphingomyelin or ceramide phosphorylethanolamine (CPE), major sphingolipids the cell vertebrates invertebrates, respectively. However, genome analyses show particularly high frequency aegerolysin genes including pathogenic genera Pseudomonas Vibrio; these human pathogens clinical...
Abstract Mitochondrial ribosomes are specialized for the synthesis of membrane proteins responsible oxidative phosphorylation. Mammalian mitoribosomes diverged considerably from ancestral bacterial and feature dramatically reduced ribosomal RNAs. Structural basis mammalian mitochondrial ribosome assembly is currently not understood. Here we present eight distinct intermediates human large mitoribosomal subunit involving 7 factors. We discover that NSUN4-MTERF4 dimer plays a critical role in...
Ribosomes have been suggested to directly control gene regulation, however regulatory roles for ribosomal RNA (rRNA) remain largely unexplored. Expansion segments (ESs) consist of a multitude tentacle-like rRNA structures that extend from the core ribosome in eukaryotes. ESs are remarkably variable sequence and size across eukaryotic evolution with unknown function. In characterizing binding element within Homeobox (Hox) 5’ UTR, we unexpectedly identify modular stem-loop this binds single...