A5085639137- Yersinia bacterium, plague, ectoparasites research
- Microbial Natural Products and Biosynthesis
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Pharmacological Effects of Natural Compounds
- RNA and protein synthesis mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant-based Medicinal Research
- Microbial Metabolism and Applications
- Protein Structure and Dynamics
- Trace Elements in Health
- Radiopharmaceutical Chemistry and Applications
- Redox biology and oxidative stress
- Viral Infections and Immunology Research
- Microbial Metabolic Engineering and Bioproduction
- Mycobacterium research and diagnosis
- Eicosanoids and Hypertension Pharmacology
- Plant biochemistry and biosynthesis
- Bacterial biofilms and quorum sensing
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Organic Chemistry Cycloaddition Reactions
- Conducting polymers and applications
- Analytical Chemistry and Sensors
- Food Chemistry and Fat Analysis
ETH Zurich
2012-2020
Institute of Molecular Biology and Biophysics
2015
Paul Scherrer Institute
2014
Helmholtz Centre for Infection Research
2011-2013
Institute of Structural and Molecular Biology
2010
Imperial College London
2010
Abstract Hepatitis C virus (HCV), a widespread human pathogen, is dependent on highly structured 5′-untranslated region of its mRNA, referred to as internal ribosome entry site (IRES), for the translation all proteins. The HCV IRES initiates by directly binding small ribosomal subunit (40S), circumventing need many eukaryotic initiation factors required mRNA scanning. Here we present cryo-EM structure 40S in complex with at 3.9 Å resolution, determined focused refinement an 80S ribosome–HCV...
Pathogens often rely on thermosensing to adjust virulence gene expression. In yersiniae, important virulence-associated traits are under the control of master regulator RovA, which uses a built-in thermosensor its activity. Thermal upshifts encountered upon host entry induce conformational changes in RovA dimer that attenuate DNA binding and render protein more susceptible proteolysis. Here, we report crystal structure free DNA-bound forms provide evidence thermo-induced loss activity is...
We demonstrate how two copper atoms are brought together to build a metal center in an essential enzyme for cellular respiration.
In enteropathogenic Yersinia, the expression of several early-phase virulence factors such as invasin is tightly regulated in response to environmental cues. The responsible regulatory network complex, involving RNAs and proteins LysR-type transcription regulator (LTTR) RovM. this study, crystal structure effector-binding domain (EBD) RovM, first LTTR protein described being involved regulation, was determined at a resolution 2.4 Å. Size-exclusion chromatography comparison with structures...
AlsR from Bacillus subtilis, a member of the LysR-type transcriptional regulator (LTTR) family, regulates transcription alsSD operon encoding enzymes involved in acetoin biosynthesis. LTTRs represent largest known family regulators bacteria. In this study, AlsR82-302S100A, representing effector domain, was produced Escherichia coli, purified and crystallized using sitting-drop vapour-diffusion method presence 2.1 M DL-malic acid pH 7.0 at 293 K. The crystals belonged to space group C2, with...
Ribosomes have been suggested to directly control gene regulation, however regulatory roles for ribosomal RNA (rRNA) remain largely unexplored. Expansion segments (ESs) consist of a multitude tentacle-like rRNA structures that extend from the core ribosome in eukaryotes. ESs are remarkably variable sequence and size across eukaryotic evolution with unknown function. In characterizing binding element within Homeobox (Hox) 5’ UTR, we unexpectedly identify modular stem-loop this binds single...