A5085281748- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- Carbon Dioxide Capture Technologies
- Biochemical and biochemical processes
- Biofuel production and bioconversion
- Photosynthetic Processes and Mechanisms
- Microbial Natural Products and Biosynthesis
- Amino Acid Enzymes and Metabolism
- Gene Regulatory Network Analysis
- Metabolism and Genetic Disorders
- Neurological diseases and metabolism
- Biochemical Acid Research Studies
- Microbial metabolism and enzyme function
- Biochemical and Molecular Research
- Mitochondrial Function and Pathology
- Microbial Community Ecology and Physiology
- Bacterial Genetics and Biotechnology
- Microbial Fuel Cells and Bioremediation
- Carbon dioxide utilization in catalysis
- Genomics and Phylogenetic Studies
- CO2 Reduction Techniques and Catalysts
- Protein Structure and Dynamics
- Metalloenzymes and iron-sulfur proteins
- Algal biology and biofuel production
Max Planck Institute for Terrestrial Microbiology
2016-2025
Loewe Center for Synthetic Microbiology
2016-2025
University of South Florida
2024
Synthetic Biologics (United States)
2022-2023
Max Planck Society
2017-2023
Philipps University of Marburg
2020-2021
ETH Zurich
2012-2016
Board of the Swiss Federal Institutes of Technology
2013-2014
University of Illinois Urbana-Champaign
2011-2012
University of Freiburg
2008-2011
Optimizing designer metabolisms in vitro Biological carbon fixation requires several enzymes to turn CO 2 into biomass. Although this pathway evolved plants, algae, and microorganisms over billions of years, many reactions could aid the production desired chemical products instead Schwander et al. constructed an optimized synthetic by using 17 enzymes—including three engineered enzymes—from nine different organisms across all domains life (see Perspective Gong Li). The is up five times more...
Significance CO 2 fixation is the most important biosynthesis process on Earth, enabling autotrophic organisms to synthesize their entire biomass from inorganic carbon at expense of energy generated by photo- or chemotrophic processes. In present study we demonstrate an autotrophy pathway that represents energy-efficient mechanism for fixing in presence oxygen. This novel variant hydroxypropionate/hydroxybutyrate cycle appears be common a ubiquitous and abundant group microorganisms can...
Fifty years ago, Kornberg and Krebs established the glyoxylate cycle as pathway for synthesis of cell constituents from C2-units. However, since then, many bacteria have been described that do not contain isocitrate lyase, key enzyme this pathway. Here, a termed ethylmalonyl-CoA operating in such organisms is described. Isotopically labeled acetate bicarbonate were transformed to by extracts acetate-grown, lyase-negative Rhodobacter sphaeroides determined NMR spectroscopy. Crotonyl-CoA...
Nature integrates complex biosynthetic and energy-converting tasks within compartments such as chloroplasts mitochondria. Chloroplasts convert light into chemical energy, driving carbon dioxide fixation. We used microfluidics to develop a chloroplast mimic by encapsulating operating photosynthetic membranes in cell-sized droplets. These droplets can be energized power enzymes or enzyme cascades analyzed for their catalytic properties multiplex real time. demonstrate how these microdroplets...
Abstract Background The biological degradation of plastics is a promising method to counter the increasing pollution our planet with artificial polymers and develop eco-friendly recycling strategies. Polyethylene terephthalate (PET) thermoplast industrially produced from fossil feedstocks since 1940s, nowadays prevalently used in bottle packaging textiles. Although established industrial processes for PET exist, large amounts still end up environment—a significant portion thereof world’s...
Abstract The capture of CO 2 by carboxylases is key to sustainable biocatalysis and a carbon-neutral bio-economy, yet currently limited few naturally existing enzymes. Here, we developed glycolyl-CoA carboxylase (GCC), new-to-nature enzyme, combining rational design, high-throughput microfluidics microplate screens. During this process, GCC’s catalytic efficiency improved three orders magnitude match the properties natural -fixing We verified our active-site redesign with an...
Optimization of biological networks is often limited by wet lab labor and cost, the lack convenient computational tools. Here, we describe METIS, a versatile active machine learning workflow with simple online interface for data-driven optimization targets minimal experiments. We demonstrate our various applications, including cell-free transcription translation, genetic circuits, 27-variable synthetic CO2-fixation cycle (CETCH cycle), improving these systems between one two orders...
Bioactive peptides are key molecules in health and medicine. Deep learning holds a big promise for the discovery design of bioactive peptides. Yet, suitable experimental approaches required to validate candidates high throughput at low cost. Here, we established cell-free protein synthesis (CFPS) pipeline rapid inexpensive production antimicrobial (AMPs) directly from DNA templates. To our platform, used deep thousands AMPs de novo. Using computational methods, prioritized 500 that produced...
Fractals are patterns that self-similar across multiple length-scales
Chemo- and stereoselective reductions are important reactions in chemistry biology, reductases from biological sources increasingly applied organic synthesis. In contrast, carboxylases used only sporadically. We recently described crotonyl-CoA carboxylase/reductase, which catalyzes the reduction of (E)-crotonyl-CoA to butyryl-CoA but also reductive carboxylation ethylmalonyl-CoA. this study, complete stereochemical course both was investigated detail. The pro-(4R) hydrogen NADPH is...
Significance Photorespiration limits plant carbon fixation by releasing CO 2 and using cellular resources to recycle the product of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) oxygenation, 2-phosphoglycolate. We systematically designed synthetic photorespiration bypasses that combine existing new-to-nature enzymatic activities do not release . Our computational model shows these could enhance rate under a range physiological conditions. To realize bypasses, glycolate reduction...
Carbon fixation is one of the most important biochemical processes. Most natural carbon pathways are thought to have emerged from enzymes that originally performed other metabolic tasks. Can we recreate emergence a pathway in heterotrophic host by recruiting only endogenous enzymes? In this study, address question systematically analyzing possible composed Escherichia coli native enzymes. We identify GED (Gnd-Entner-Doudoroff) cycle as simplest can operate with high thermodynamic driving...
The efficient regeneration of cofactors is vital for the establishment biocatalytic processes. Formate an ideal electron donor cofactor due to its general availability, low reduction potential, and benign byproduct (CO2). However, formate dehydrogenases (FDHs) are usually specific NAD+, such that NADPH with challenging. Previous studies reported naturally occurring FDHs or engineered accept NADP+, but these enzymes show kinetic efficiencies specificities. Here, we harness power natural...