A5051542402- Bacterial biofilms and quorum sensing
- Enzyme Structure and Function
- Enzyme Production and Characterization
- Paraoxonase enzyme and polymorphisms
- Bacterial Genetics and Biotechnology
- Pesticide and Herbicide Environmental Studies
- Enzyme Catalysis and Immobilization
- Coenzyme Q10 studies and effects
- Amino Acid Enzymes and Metabolism
- Biochemical and Molecular Research
- Antibiotic Resistance in Bacteria
- Drug Transport and Resistance Mechanisms
- Biochemical Acid Research Studies
- Environmental Toxicology and Ecotoxicology
- Pesticide Exposure and Toxicity
- Biochemical and Structural Characterization
- Enzyme function and inhibition
- Corrosion Behavior and Inhibition
- Cultural Heritage Materials Analysis
- Chemical Reaction Mechanisms
- Cancer-related gene regulation
- Antimicrobial Resistance in Staphylococcus
- Protein Structure and Dynamics
- Cancer, Hypoxia, and Metabolism
- Mass Spectrometry Techniques and Applications
Biotechnology Institute
2016-2025
University of Minnesota
2016-2025
Twin Cities Orthopedics
2018-2020
Méditerranée Infection Foundation
2020
Los Alamos National Laboratory
2016
University of Trnava
2016
Weizmann Institute of Science
2010-2015
Architecture et Fonction des Macromolécules Biologiques
2007-2010
Aix-Marseille Université
2010
Centre National de la Recherche Scientifique
2005-2009
Pseudomonas aeruginosa is a Gram negative pathogenic bacterium involved in many human infections including otitis, keratitis, pneumonia, and diabetic foot ulcers. P. uses communication system, referred to as quorum sensing (QS), adopt group behavior by synchronizing the expression of certain genes. Among regulated traits, secretion proteases or siderophores, motility biofilm formation are mainly pathogenicity. Many efforts have been dedicated development inhibitors (QSI) quenching (QQ)...
The effectiveness of antibiotic molecules in treating Pseudomonas aeruginosa pneumonia is reduced as a result the dissemination bacterial resistance. existence communication systems, such quorum sensing, has provided new opportunities treatment. Lactonases efficiently quench acyl-homoserine lactone-based implicating these enzymes potential anti-Pseudomonas drugs that might be evaluated pneumonia.The aim present study was to evaluate ability lactonase called SsoPox-I reduce mortality rat P....
Enzymes are proficient catalysts that enable fast rates of Michaelis-complex formation, the chemical step and products release. These different steps may require conformational states active site have distinct binding properties. Moreover, flexibility mediates alternative, promiscuous functions. Here we focused on lactonase SsoPox from Sulfolobus solfataricus. is a native endowed with phosphotriesterase activity. We identified position in loop (W263) governs its flexibility, thereby affects...
SsoPox is a lactonase endowed with promiscuous phosphotriesterase activity isolated from Sulfolobus solfataricus that belongs to the Phosphotriesterase-Like Lactonase family. Because of its intrinsic thermal stability, seen as an appealing candidate bioscavenger for organophosphorus compounds. A comprehensive kinetic characterisation has been performed various phosphotriesters (insecticides) and phosphodiesters (nerve agent analogues) substrates. We show active broad range OPs remains under...
Background A new member of the Phosphotriesterase-Like Lactonases (PLL) family from hyperthermophilic archeon Sulfolobus islandicus (SisLac) has been characterized. SisLac is a native lactonase that exhibits high promiscuous phosphotriesterase activity. thus represents promising target for engineering studies, exhibiting both detoxification and bacterial quorum quenching abilities, including human pathogens such as Pseudomonas aeruginosa. Methodology/Principal Findings Here, we describe...
Metformin is the first-line treatment for type II diabetes patients and a pervasive pollutant with more than 180 million kg ingested globally entering wastewater. The drug's direct mode of action currently unknown but linked to effects on gut microbiomes may involve specific microbial reactions drug. In wastewater plants, metformin known be transformed by microbes guanylurea, although genes encoding this metabolism had not been elucidated. present study, we revealed function two responsible...
Background Organophosphates (OPs) are neurotoxic compounds for which current methods of elimination unsatisfactory; thus bio-remediation is considered as a promising alternative. Here we provide the structural and enzymatic characterization recently identified enzyme isolated from Pseudomonas pseudoalcaligenes dubbed OPHC2. OPHC2 belongs to metallo-β-lactamase superfamily exhibits an unusual thermal resistance some OP degrading abilities. Principal findings The X-ray structure has been...
Constrained, cyclic peptides encoded by plant genes represent a new generation of drug leads. Evolution has repeatedly recruited the Cys-protease asparaginyl endopeptidase (AEP) to perform their head-to-tail ligation. These macrocyclization reactions use substrates amino terminus instead water deacylate, so peptide bond is formed. How solvent-exposed AEPs macrocyclize poorly understood. Here we present crystal structure an active AEP from common sunflower, Helianthus annuus. The site...
Quorum quenching lactonases are enzymes that capable of disrupting bacterial signaling based on acyl homoserine lactones (AHL) via their enzymatic degradation. In particular, have therefore been demonstrated to inhibit behaviors depend these chemicals, such as the formation biofilms or expression virulence factors. Here we characterized biochemically and structurally a novel representative from metallo-β-lactamase superfamily, named AaL was isolated thermoacidophilic bacterium...