A5055604616- Microbial Metabolic Engineering and Bioproduction
- Metabolomics and Mass Spectrometry Studies
- Microbial metabolism and enzyme function
- Enzyme Catalysis and Immobilization
- Amino Acid Enzymes and Metabolism
- Biofuel production and bioconversion
- Analytical Chemistry and Chromatography
- Gut microbiota and health
- Mass Spectrometry Techniques and Applications
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Plant-Microbe Interactions and Immunity
- Lipoproteins and Cardiovascular Health
- Blood Coagulation and Thrombosis Mechanisms
- Salmonella and Campylobacter epidemiology
- Bacterial Genetics and Biotechnology
- Microbial Natural Products and Biosynthesis
- Biochemical and Molecular Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antimicrobial Resistance in Staphylococcus
- Gene Regulatory Network Analysis
- Legionella and Acanthamoeba research
- Enzyme Structure and Function
- Mitochondrial Function and Pathology
- Microbial bioremediation and biosurfactants
ETH Zurich
2015-2025
University of Milan
2006-2024
Laboratory Dr. Wisplinghoff
2017
Board of the Swiss Federal Institutes of Technology
2007-2015
University of Zurich
2010
Saarland University
2002-2007
Centre National de la Recherche Scientifique
2007
Université Toulouse III - Paul Sabatier
2007
Université de Picardie Jules Verne
2007
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
2007
The association of arbuscular mycorrhizal (AM) fungi with plant roots is the oldest and ecologically most important symbiotic relationship between higher plants microorganisms, yet mechanism by which these detect presence a host poorly understood. Previous studies have shown that secrete branching factor (BF) strongly stimulates hyphae during germination spores AM fungi. In BF Lotus, strigolactone was found to be active molecule. Strigolactones are known as stimulants parasitic Striga...
In the present work we investigated most commonly applied methods used for sampling of microorganisms in field metabolomics order to unravel potential sources error previously ignored but utmost importance accurate metabolome analysis. To broaden significance our study, different Gram-negative and Gram-positive bacteria, i.e., Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli, Gluconobacter oxydans, Pseudomonas putida, Zymononas mobilis, analyzed metabolites from catabolic...
The assimilation of one-carbon (C1) compounds, such as methanol, by serine cycle methylotrophs requires the continuous regeneration glyoxylate. Instead glyoxylate cycle, this process is achieved a not yet established pathway where CoA thioesters are known to play key role. We applied state-of-the-art metabolomics and 13 C strategies demonstrate how generated during methylotrophic growth in isocitrate lyase-negative methylotroph Methylobacterium extorquens AM1. High-resolution mass...
A comprehensive approach to (13)C tracer studies, labeling measurements by gas chromatography-mass spectrometry, metabolite balancing, and isotopomer modeling, was applied for comparative metabolic network analysis of lysine-producing Corynebacterium glutamicum on glucose or fructose. Significantly reduced yields lysine biomass enhanced formation dihydroxyacetone, glycerol, lactate in comparison those resulted Metabolic flux revealed drastic differences intracellular depending the carbon...
Methanol represents an attractive substrate for biotechnological applications. Utilization of reduced one-carbon compounds growth is currently limited to methylotrophic organisms, and engineering synthetic methylotrophy remains a major challenge. Here we apply in silico-guided multiple knockout approach engineer methanol-essential Escherichia coli strain, which contains the ribulose monophosphate cycle methanol assimilation. conversion biomass was stoichiometrically coupled metabolization...
Methylotrophic microorganisms are playing a key role in biogeochemical processes - especially the global carbon cycle and have gained interest for biotechnological purposes. Significant progress was made recent years biochemistry, genetics, genomics, physiology of methylotrophic bacteria, showing that methylotrophy is much more widespread versatile than initially assumed. Despite such progress, system-level description metabolism currently lacking, remains to understand regarding...
The sustainable production of industrial platform chemicals is one the great challenges facing biotechnology field. Ideally, fermentation feedstocks would rather rely on waste streams than food-based raw materials. Corynebacterium glutamicum was metabolically engineered to produce bio-nylon precursor 1,5-diaminopentane from hemicellulose sugar xylose. Comparison a basic diaminopentane producer strain xylose and glucose revealed 30% reduction in yield productivity pentose sugar. integration...
Single-cell metabolite analysis provides valuable information on cellular function and response to external stimuli. While recent advances in mass spectrometry reached the sensitivity required investigate metabolites single cells, current methods commonly isolate sacrifice inflicting a perturbed state preventing complementary analyses. Here, we propose two-step approach that combines nondestructive quantitative withdrawal of intracellular fluid with subpicoliter resolution using fluidic...
Methanol is a liquid with high energy storage capacity that holds promise as an alternative substrate to replace sugars in the biotechnology industry. It can be produced from CO2 or methane and its use does not compete food animal feed production. However, there are currently only limited biotechnological options for valorization of methanol, which hinders widespread adoption. Here, we report conversion industrial platform organism Escherichia coli into synthetic methylotroph assimilates...
Foodborne bacterial diarrhea involves complex pathogen-microbiota-host interactions. Pathogen-displacing probiotics are increasingly popular, but heterogeneous patient outcomes highlighted the need to understand individualized host-probiotic activity. Using mouse gut commensal Escherichia coli 8178 and human probiotic E. Nissle 1917, we found that degree of protection against enteric pathogen Salmonella enterica serovar Typhimurium (S. Tm) varies across mice with distinct microbiotas....
Abstract Endotoxin-driven systemic immune activation is a common hallmark across various clinical conditions. During acute critical illness, elevated plasma lipopolysaccharide triggers non-specific activation. In addition, compositional shift in the gut microbiota, including an increase gut-luminal opportunistic pathogens, observed. Whether causal link exists between endotoxemia and abundance of pathogens incompletely understood. Here, we model acute, pathophysiological concentrations mice...
The opportunistic pathogen Legionella pneumophila replicates in human lung macrophages and free-living amoebae. To accommodate the transfer between host cells, L. switches from a replicative to transmissive phase. harbors gene cluster homologous Vibrio cholerae cqsAS quorum sensing system, encoding putative autoinducer synthase (lqsA) sensor kinase (lqsS), which flank response regulator (lqsR). LqsR is an element of virulence regulatory network, promotes pathogen-host cell interactions...
Methanol dehydrogenase-like protein XoxF of Methylobacterium extorquens AM1 exhibits a sequence identity 50 % to the catalytic subunit MxaF periplasmic methanol dehydrogenase in same organism. The latter has been characterized detail, identified as pyrroloquinoline quinone (PQQ)-dependent protein, and shown be essential for growth presence this methylotrophic model bacterium. In contrast, function M. not yet elucidated, phenotype remained described xoxF mutant. Here, we found that mutant is...
The bacterial isolate GFAJ-1 has been proposed to substitute arsenic for phosphorus sustain growth. We have shown that is able grow at low phosphate concentrations (1.7 μM), even in the presence of high arsenate (40 mM), but lacks ability phosphorus-depleted (<0.3 arsenate-containing medium. High-resolution mass spectrometry analyses revealed phosphorylated central metabolites and nucleic acids predominated. A few arsenylated compounds, including C6 sugar arsenates, were detected extracts...
Faster growing and more virulent strains of methicillin resistant Staphylococcus aureus (MRSA) are increasingly displacing highly MRSA. Elevated fitness in these MRSA is often accompanied by decreased heterogeneous levels resistance; however, the mechanisms for this phenomenon not yet fully understood. Whole genome sequencing was used to investigate genetic basis apparent correlation, an isogenic strain pair that differed resistance fitness, with respect growth rate. Sequencing revealed only...
In this article, we introduce a method using nanoscale ion-pair reversed-phase high-performance liquid chromatography (nano-IP-RP-HPLC) hyphenated to nanoelectrospray ionization high-resolution mass spectrometry (nano-ESI-HRMS) separate and identify metabolites in cell extracts. Separation of was performed on 100 μm i.d. C18 column with tributylamine (TBA) as the ion-pairing reagent methanol eluent. Basic pH (9.4) mobile phase critical achieve sufficient retention sharp metabolite elution at...
Significance Shigella causes devastating bloody diarrhea. Rapid disease progression results from exceptionally fast growth inside human gut epithelial cells, but how can obtain nutrients at such high rates its host cell has been unclear. Here, we show that infected cells maintain normal central metabolism for energy production and survival. However, captures the entire output degrades it further to acetate. This striking strategy provides with an abundant supply of a favorable source, while...
Abstract Background Bacteria of the genus Arthrobacter are ubiquitous in soil environments and can be considered as true survivalists. sp. strain Rue61a is an isolate from sewage sludge able to utilize quinaldine (2-methylquinoline) sole carbon energy source. The genome provides insight into molecular basis versatility robustness this environmental strain. Results consists a single circular chromosome 4,736,495 bp with average G + C content 62.32%, 231,551-bp plasmid pARUE232, linear...