A5077116472- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Diagnosis and treatment of tuberculosis
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Infectious Diseases and Tuberculosis
- Escherichia coli research studies
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- Clostridium difficile and Clostridium perfringens research
- Drug Transport and Resistance Mechanisms
- Sphingolipid Metabolism and Signaling
- Vector-borne infectious diseases
- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Mosquito-borne diseases and control
- Erythrocyte Function and Pathophysiology
- Viral Infections and Vectors
- Cancer therapeutics and mechanisms
University of Alabama at Birmingham
2015-2025
National Agricultural Technology Institute
2004-2012
Czech Academy of Sciences, Institute of Biotechnology
2009
Instituto de Biotecnología y Biología Molecular
2005-2006
Iron is essential for nearly all bacterial pathogens, including Mycobacterium tuberculosis (Mtb), but severely limited in the human host. To meet its iron needs, Mtb secretes siderophores, small molecules with high affinity iron, and takes up iron-loaded mycobactins (MBT) carboxymycobactins (cMBT), from environment. also capable of utilizing heme hemoglobin which contain more than 70% body. However, many components these acquisition pathways are still unknown. In this study, a high-density...
Sphingomyelinases secreted by pathogenic bacteria play important roles in host-pathogen interactions ranging from interfering with phagocytosis and oxidative burst to iron acquisition. This study shows that the Mtb protein Rv0888 possesses potent sphingomyelinase activity cleaving sphingomyelin, a major lipid eukaryotic cells, into ceramide phosphocholine, which are then utilized as carbon, nitrogen phosphorus sources, respectively. An rv0888 deletion mutant did not grow on sphingomyelin...
ABSTRACT Bovine tuberculosis is a major problem in many countries; hence, new and better diagnostic tools are urgently needed. In this work, we have tested ESAT6, CFP10, PE13, PE5, MPB70, TB10.4, TB27.4 for their potentials as markers field animals from Northern Ireland, Mexico, Argentina, regions with low, medium, high prevalences of bovine tuberculosis, respectively. At all three sites, ESAT6 CFP10 were superior antigens, while combination performed even at the two sites where was tested,...
Drug-resistant
ABSTRACT Tuberculosis is the leading cause of death from a single infectious disease. Mycobacterium tuberculosis secretes proteins using five ESX systems with distinctive functions essential for its growth and virulence. Here we show that non-canonical supercomplex EsxU-EsxT proteins, encoded in esx-4 locus, orphan EsxE-EsxF cpnT operon, required toxin secretion by M. . Surprisingly, outer membrane localization all Esx their into cytosol infected macrophages also depend on EsxEF-EsxUT ESX-4....
The confirmatory diagnosis of Mycobacterium bovis ( M. bovis) in animal samples is carried out by culture Stonebrink media. However, very slow because the extremely long duplication time bacillus and difficult scarcity bacilli diagnostic samples. This study describes development a single-tube touch-down polymerase chain reaction (PCR) protocol for detection using primers that target IS 6110 element. Spiked water milk as well routine (milk nasal swabs) from bovis–positive cattle were tested....
ABSTRACT A number of studies have determined the contribution Th1 and Th2 responses to protective immunity pathology Mycobacterium bovis infection. However, much that information is derived from experimentally infecting cattle with M. few data naturally infected animals are available. The aim this study was characterize immunological profile towards antigens by measurement cytokine mRNA expression in PBMC, determine which lymphocyte subsets involved recall PBMC antigens. Consistent , were...
Abstract Background In many regions of the world, wild mammals act as reservoir Mycobacterium bovis , a situation that prevents eradication bovine tuberculosis. order to observe whether strain isolated from boar, previously tested highly virulent in mice model, is also cattle, we performed cattle experimental inoculation with this Results Groups Friesian calves were either infected boar M. 04-303 or NCTC10772 control. We found antigen-specific IFN-γ release whole blood samples occurred...
The generation of efficient candidate vaccines against bovine tuberculosis will contribute to the control this zoonotic disease. Rationally attenuated Mycobacterium bovis strains generated by knockout virulence genes are promising vaccines. However, be effective, these should at least maintain immunological properties their virulent parental M. strains. Therefore, aim study was obtain an strain deleted in mce2 and evaluate effect mutation on profile elicited bacteria cattle. We showed that...
Mycobacterium tuberculosis, the causative agent of human tuberculosis (TB), has surpassed HIV/AIDS as leading cause death from a single infectious agent. The increasing occurrence drug-resistant strains become major challenge for health care systems and, in some cases, rendered TB untreatable. However, development new drugs been plagued with high failure rates and costs. Alternative strategies to increase efficacy current treatment regimens include host-directed therapies or agents that make...
Summary Cattle are the host and main reservoir of etiologic agent bovine tuberculosis, Mycobacterium bovis ; although other mammalian species, including humans, susceptible. The tuberculin test and/or slaughterhouse surveillance is diagnostic method used by control programs all around world to eradicate disease. In order compare different tuberculosis tests reach disease confirmation, a study was performed in group 14 steers Friesian breed, reacting positively test. Three ante‐mortem assays...
ABSTRACT Culture filtrate and cell extracts from Mycobacterium bovis cultures contain molecules which could promote protective immunity to tuberculosis in animals. Different protein fractions of M. were obtained by elution electrophoresis tested experimentally infected cattle. The that elicited gamma interferon (IFN-γ) responses resolved two-dimensional gel electrophoresis, individual proteins identified matrix-assisted laser desorption ionization-time flight mass spectrometry. open reading...
Summary A Mycobacterium avium subsp. paratuberculosis expression library in lambda ZAP was screened with immunized mice sera. One clone selected, sequenced and further characterized. The sequence analysis of the hypothetical open‐reading frame (ORF) predicts a protein 20.8 kDa probable signal compatible Cys‐acylation at Cys24, characteristic lipoproteins. In consequence, termed Lpp34. Recombinant Lpp34 achieved by cloning lpp34 gene into histidine‐tag vector pRSET‐A. Western blot showed band...