A5103252185- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Microbial infections and disease research
- Infectious Diseases and Mycology
- Ginseng Biological Effects and Applications
- Pharmacological Effects of Natural Compounds
- Bacteriophages and microbial interactions
- Veterinary medicine and infectious diseases
- Fungal Biology and Applications
- Infectious Diseases and Tuberculosis
- Educational and Social Studies
- Aquaculture disease management and microbiota
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Zoonotic diseases and public health
- Cytomegalovirus and herpesvirus research
- Psychological Treatments and Disorders
- Developmental and Educational Neuropsychology
- Immune Cell Function and Interaction
- Parasitic Infections and Diagnostics
- Bacterial Identification and Susceptibility Testing
- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Milk Quality and Mastitis in Dairy Cows
- Italian Literature and Culture
Consejo Nacional de Investigaciones Científicas y Técnicas
2007-2024
National Agricultural Technology Institute
2009-2023
Instituto de Agrobiotecnología del Litoral
2020-2023
Agrobiotechnology Institute
2022
Instituto de Biotecnología y Biología Molecular
2005-2022
Università degli Studi Suor Orsola Benincasa
2017-2020
Centro Científico Tecnológico - San Juan
2017-2018
National Institute of Industrial Technology
2002-2014
Biotechnology Institute
2011
Universidad Nacional de Asunción
2011
A comparison of Mycobacterium tuberculosis complex isolates from seals (pinnipeds) in Australia, Argentina, Uruguay, Great Britain and New Zealand was undertaken to determine their relationships each other taxonomic position within the complex. Isolates 30 cases six species pinniped seven related were compared representative standard strains M. The seal could be distinguished members complex, including recently defined 'Mycobacterium canettii' caprae', on basis host preference phenotypic...
Summary Paratuberculosis (Ptbc) has a high prevalence in Argentina, that affects dairy and beef cattle. The culture is the gold standard to diagnosis of disease. Mycobacterium avium ssp. paratuberculosis (M. paratuberculosis) , aetiological agent, difficult isolate grow culture. In this study, 24 randomly selected cows Fresian breed from herd with history Ptbc were used evaluate performance different diagnostic techniques. These animals did not show clinical signs However, another animal...
The confirmatory diagnosis of Mycobacterium bovis ( M. bovis) in animal samples is carried out by culture Stonebrink media. However, very slow because the extremely long duplication time bacillus and difficult scarcity bacilli diagnostic samples. This study describes development a single-tube touch-down polymerase chain reaction (PCR) protocol for detection using primers that target IS 6110 element. Spiked water milk as well routine (milk nasal swabs) from bovis–positive cattle were tested....
Ruminant infection by Mycobacterium avium subsp. paratuberculosis (MAP) causes a granulomatous inflammatory response in the intestine and associated lymph nodes. Differences either affected organs or infiltrate were observed between species individuals. Such differences are usually attributed to variations host immune responses inconsistent effects among different MAP strains. To evaluate if strains induce immuno-pathological lambs, 28 one-month-old individuals divided into six groups...
Mycobacterium avium sp. (MAA), M. hominissuis (MAH), and paratuberculosis (MAP) are the main members of complex (MAC) causing diseases in several hosts. The aim this study was to describe genetic diversity MAC isolated from different Twenty-six MAH 61 MAP isolates were recovered humans cattle, respectively. GenoType CM® IS1311-PCR used identify species. IS901-PCR differentiate between MAA, while IS900-PCR MAP. Genotyping performed using a mycobacterial interspersed...
ABSTRACT The presence of a 500-bp fragment which amplifies region from the genome Mycobacterium bovis (J. G. Rodriguez, A. Meija, P. Del Portillo, M. E. Patarroyo, and L. Murillo, Microbiology 141:2131–2138, 1995) was evaluated by carrying out PCR on 121 isolates. strains, previously characterized culture biochemical tests, were isolated cattle in different regions Argentina, Mexico, Colombia. Four additional strains sea lions that belong to tuberculosis complex also included study. All...
The aim of this study was to evaluate a wide panel antigens Mycobacterium avium subsp. paratuberculosis (MAP) select candidates for the diagnosis (PTB). A total 54 recombinant proteins were spotted onto nitrocellulose membranes and exposed sera from animals with PTB (n = 25), healthy 10), experimentally infected M. bovis 8). This initial screening allowed us seven antigens: MAP 2513, 1693, 2020, 0038, 1272, 0209c, 0210c, which reacted showed little cross-reactivity bovis. second step antigen...
Multiple-locus variable number-tandem repeat analysis (MLVA) of Mycobacterium avium subspecies paratuberculosis (MAP) isolates may contribute to the knowledge strain diversity in Argentina. Although MAP has been previously investigated Argentina using IS900-RFLP, a small number were employed, and low discriminative power was reached. The aim present study test genetic among an MLVA approach based on 8 repetitive loci. We studied 97 from cattle, goat sheep could describe 7 different patterns:...
Tuberculosis (TB) was diagnosed in 10 seals from three species (Arctocephalus australis, Arctocephalus tropicalis and Otaria flavescens) found South America. The mycobacteria isolated these cases belonged to the Mycobacterium tuberculosis complex, as determined by RFLP using an IS6110 probe, spoligotyping, analysis of 16S rRNA gene sequence PCR-restriction hsp65. Polymorphisms gyrA, katG, oxyR pncA were investigated some isolates, well presence MPB70 antigen. insertion present seven copies...
Neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. surface antigen 1 (Nc-SAG1) immunodominant candidate for the development of diagnostic reagent neosporosis. The current study describes and evaluation print immunoassay (APIA) with recombinant Nc-SAG1 detection specific antibodies to N. concordance between APIA commercial enzyme-linked immunosorbent assay (ELISA) was evaluated 232 serum samples from experimentally naturally infected Sixty-one...
Bovine tuberculosis is still rife in Latin America, producing huge economic losses. There are very few studies of the way this disease spread through geographical region, particularly countries that border those almost free Mycobacterium bovis. In work, we have analyzed spacer oligonucleotide typing (spoligotype) patterns M. bovis isolates from cattle at Ciudad Juárez, a Mexican city close to El Paso, Texas. Fifty-eight collected herd Northern Mexico were studied by spoligotyping. Nine...
The binding and ingestion of Mycobacterium avium subsp. paratuberculosis (MAP) by host cells are fibronectin (FN) dependent. In several species mycobacteria, a specific family proteins allows the attachment internalization these bacteria epithelial through interaction with FN. Thus, identification adhesion molecules is essential to understand pathogenesis MAP. aim this study was identify characterize FN cell wall We searched for conserved adhesins within large panel surface immunogenic MAP...