A5042361828- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Mycology
- Microbial infections and disease research
- Diagnosis and treatment of tuberculosis
- Cardiac, Anesthesia and Surgical Outcomes
- Enhanced Recovery After Surgery
- Abdominal Surgery and Complications
- RNA Interference and Gene Delivery
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Ginseng Biological Effects and Applications
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Nutrition, Genetics, and Disease
- Rabies epidemiology and control
- Infectious Diseases and Tuberculosis
- Pharmacological Effects of Natural Compounds
- Intimate Partner and Family Violence
- Advanced Biosensing Techniques and Applications
- T-cell and B-cell Immunology
- Building materials and conservation
- Paleopathology and ancient diseases
- Acute Kidney Injury Research
Universidade Federal do Paraná
2024
Charles University
2021
University Hospital Hradec Králové
2021
St James's University Hospital
2019-2020
University of Leeds
2019-2020
University of Veterinary Sciences Brno
2007-2014
Veterinary Research Institute
2001-2007
BioVendor (Czechia)
2003
Fifty one products of dried milk baby food purchased from 10 producers seven countries available on the Czech market have been tested. IS900, specific fragments for Mycobacterium avium subsp. paratuberculosis (MAP) detected using PCR in 25 samples (49.0 %) and fragment f57 by real time 18 (35.3%). These results correspond to epidemiological situation Europe are not unexpected. Paratuberculosis cattle was almost unknown Republic until 1990. An increase number cows with found slaughterhouses...
Sources of mycobacterial infections in 50 pig herds the Czech Republicwere investigated during years 1996 to 2002. A total 2 412 samples from external environment (feeds, bedding materials, drinking water, biofilms on drinkers, scrapings walls, floors and pen barriers, dust, spider webs, peat, kaolin, faeces, organs rodents, birds, etc.) were examined. After staining by Ziehl-Neelsen technique, acid-fast rods detected 95 (3.9%) direct microscopic examination mycobacteria cultured 575 (23.8%)...
In the first part of study, 70 aquarium fish different species, originating from stocks CzechRepublic, were pathomorphologically examined for presence granulomatous lesions. Granulomas diagnosed in 44 (62.9%) fish. From these, acid fast rods (AFR) found 29 (65.9%) by staining according to Ziehl-Neelsen (Z-N). remaining 26 (37.1%) without lesions, AFR was microscopically proven 3 (11.5%) second direct microscopic examinations according Z-N and culture mycobacteria carried out 17 randomly...
Molecular biology methods offer new opportunities to differentiate, identify and type bacterial species strains. These use the variability of nucleic sequences genes such as 16S rDNA, beta subunit RNA-ase (rpoB), gyrase (gyrB), rDNA internal transcribed spacer other genes. The aim this paper is provide comprehensive information about available differentiate mycobacteria at DNA sequence level. discussed in review include PCR, PCR-REA, sequencing analysis, spoligotyping fingerprinting. have...
Spoligotyping was used to examine IS6110-positive DNA of 26 Mycobacterium bovis, M. bovis BCG and subsp. caprae non-viable isolates stored up 10 years. All these were previously identified by biochemical tests all 17/17 tested earlier found virulent for guinea pigs. In total seven spoligotypes, designated S1-S7, detected compared with the spoligotypes 3 176 in database National Institute Public Health Environment (RIVM) Bilthoven, Netherlands. A Neotype strain, isolated 1965 USA thereafter...
The last outbreak of bovine tuberculosis in cattle the CzechRepublic was detected 1995. Signs diarrhoea, weight loss and occasional coughing appeared one 14-year-old cow after giving birth for thirteenth time. Two months these symptoms had been observed, it to be slaughtered numerous tuberculous lesions were found its lung tissue, including pleura. Within three confirmation infection consecutive intra-vitam post-mortem diagnostics, all 28 remaining head from herd (nine cows, seven bulls, six...
The presence of mycobacteria was studied in Bull Rock Cave ("Byci skala") and the water catchment area Jedovnice Brook ("Jedovnicky potok") using direct microscopy after Ziehl-Neelsen (ZN) staining, culture examination molecular techniques. Mycobacteria were detected 47.1% a total 68 samples. mycobacterial genes hsp65 dnaA sequenced 37 (74.0%) out 50 cave environmental samples 10 (55.6%) 18 sediments. Nine species slowly growing (M. terrae, M. arupense, gordonae, lentiflavum,...
Summary A total of 24 Pasteurella multocida rabbit isolates obtained from flocks in the Czech Republic during period between 2001 and 2004 were analysed by capsular PCR typing. Apart identified as serogroups ( n = 14, 58.4%) D 2, 8.3%), eight (33.3%) members serogroup F. This had been predominantly associated with poultry infections so far. The F characterized detail ribotyping restriction to endonuclease Msp I revealing two distinct ribotypes. Seven assigned ribotype 1 one isolate was 2.
Summary In the autumn of 2004, tuberculosis caused by Mycobacterium caprae occurred in a zoo Slovenia. A dromedary camel ( Camelus dromedarius ) was killed after history progressive emaciation. Necropsy findings indicated disseminated tuberculosis, which confirmed cultivation M. . Consequently, tuberculin skin test performed all epidemiologically linked animals and another six bison Bison were positive killed. isolated from two while scrofulaceum spp. found other bison, respectively. The...
Abstract. The syrphid Eristalis tenax Linnaeus (Diptera: Syrphidae) may be found in and around dung storage pits at cattle farms various developmental stages of their life cycle. purpose this study was to investigate the occurrence Mycobacterium avium ssp. paratuberculosis 1044 E. samples stages, as well fresh stored originating from nine farms. fortuitum isolated one (1.5%) larva vicinity three paratuberculosis‐free herds cattle. a . 111 (21.4%) larvae collected two seven known infected...
In Norway, paratuberculosis has been frequently diagnosed in goats, while cattle have almost free of the infection. This difference prevalence between goats and led to speculations about existence a Mycobacterium avium subsp. (M. a. paratuberculosis) isolate that is non-pathogenic for cattle. There little information available on genotypic variation M. isolated from animals Norway. present study, 51 isolates four Norway was obtained by use IS900 restriction fragment length polymorphism...
A total of 842 wild boar differing ages, originating from 29 (37.7%) the 77 districts in Czech Republic, were examined during hunting seasons 2002 to 2005. Of them, 274 (32.5%) animals specimens and 568 (67.5%) game parks. Out 786 animals, following included study: 668 piglets, 61 juveniles, 32 adult males 25 females. 2 704 samples various tissues faeces examined: 309 separately collected faecal (36.7%) 332 63 533 (63.3%) animals. Mycobacteria isolated 75 (8.9%) 11 districts. Neither a...