A5077791618- Streptococcal Infections and Treatments
- Herpesvirus Infections and Treatments
- Animal Disease Management and Epidemiology
- Microbial infections and disease research
- Vector-Borne Animal Diseases
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Mycobacterium research and diagnosis
- Milk Quality and Mastitis in Dairy Cows
- Bacterial Identification and Susceptibility Testing
- Toxoplasma gondii Research Studies
- T-cell and Retrovirus Studies
- Viral gastroenteritis research and epidemiology
- Infective Endocarditis Diagnosis and Management
- Diphtheria, Corynebacterium, and Tetanus
- Muscle metabolism and nutrition
- Microbial Metabolism and Applications
- Infectious Diseases and Mycology
- Animal health and immunology
- Cytomegalovirus and herpesvirus research
- Plant Virus Research Studies
- Viral Infections and Vectors
- Neonatal and Maternal Infections
- HIV Research and Treatment
- Antimicrobial Resistance in Staphylococcus
Università di Camerino
2013-2023
Servicio Regional de Investigación y Desarrollo Agroalimentario
2018
University of Pisa
2002-2009
In order to fulfil the seroprevalence gap on Mycobacterium avium subsp. paratuberculosis infection in ovine dairy farms of Marche region (central Italy), a stratified study was carried out 2086 adult female sheep randomly chosen from 38 herds selected Ancona and Macerata provinces. 73.7% flocks resulted infected by commercial ELISA test (Pourquier, France), with mean 6.29% sampled both A higher number MAP seropositive ewes recorded large herds' consistence than small medium (), greater...
The equine disease strangles, which is characterized by the formation of abscesses in lymph nodes head and neck, one most frequently diagnosed infectious diseases horses around world. causal agent, Streptococcus equi subspecies equi, establishes a persistent infection approximately 10 % animals that recover from acute disease. Such 'carrier' appear healthy are rarely identified during routine veterinary examinations pre-purchase or transit, but can transmit S. to naïve initiating new...
A morphological, immunohistochemical and polymerase chain reaction (PCR) study was performed on eight ewes experimentally infected with an Italian strain of Maedi-Visna Virus (MVV) in order to evaluate the lesions viral distribution after three years infection. At moment euthanasia, seven sheep were seropositive for MVV, while one poor body conditions seronegative since year. Lungs, pulmonary lymph nodes, udder, supramammary carpal joints, CNS, spleen bone marrow collected histology,...
ABSTRACT Streptococcus canis is an animal pathogen that occasionally causes human infections. Isolates recovered from infections of animals ( n = 78, 2000 to 2010 in three European countries, mainly house pets) and humans 7, 2006 Portugal) were identified by phenotypic genotypic methods characterized antimicrobial susceptibility testing, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), emm typing. S. isolates presented considerable variability biochemical profiles...
Maedi Visna Virus (MVV) is the etiological agent of a systemic disease sheep, which causes lesions in lungs, central nervous system, joints, and mammary glands. It has been speculated that association with Brucella ovis may lead to venereal shedding virus. In this work, samples epididymis from ten rams positive for MVV infected experimentally ovis, were subjected liquid- phase PCR, immunohistochemistry (IHC) situ PCR tests, aimed at identifying pathogens tissue context. IHC was carried out...
Abstract Japanese Encephalitis Virus (JEV) is a flavivirus responsible for an important zoonotic, vector‐borne disease included in the OIE list. JEV endemic large area of Asia. In Italy, has been found dead birds collected 1997–2000 and pool Culex pipiens mosquitoes 2010. Viral ecology inter‐epidemic periods not known. The aim this study was to investigate FFPE archival samples healthy Tuscany (Italy) same few months after collection resulted infected by JEV. Different from 37 young 83...
Streptococcus (S.) dysgalactiae subsp. equisimilis is responsible for severe diseases in humans, including primary bacteraemia, pneumonia, endocarditis, and toxic shock syndrome. Infection some animal species can also occur, although a few studies have looked into cross-species infectivity. In horses, S. generally considered infrequent or opportunistic, but has recently been isolated from cases of strangles-like disease. Rapid sensitive diagnostic techniques could enable epidemiological...
The pathogenic role of beta-hemolytic Streptococcus dysgalactiae in the equine host is increasingly recognized. A collection 108 Lancefield group C (n = 96) or L 12) horse isolates recovered United States and three European countries presented multilocus sequence typing (MLST) alleles, types emm (only 56% could be typed) that were, with few exceptions, distinct from those previously found human subsp. equisimilis. Characterization a subset by analysis (MLSA) 16S rRNA gene showed most also...
Stainless steel, widely present in the food industry, is frequently exposed to bacterial colonization with possible consequences on consumers’ health. 288 stainless steel disks different roughness (0.25, 0.5 and 1 μm) were challenged four Gram-negative (Escherichia coli ATCC 25922, Salmonella typhimurium 1402, Yersinia enterocolitica 9610 Pseudomonas aeruginosa 27588) Gram-positive bacteria (Staphylococcus aureus 6538, Enterococcus faecalis 29212, Bacillus cereus 14579 Listeria monocytogenes...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the pandemic COVID-19. The virus infects human cells by binding of spike to cell receptor ACE2. crystal structure SARS-CoV-2 spikes in complex with ACE2 has recently been solved, and main amino acid residues involved virus–receptor have detected. To investigate affinity lagomorphs spike, sequences from rabbits American pikas were compared mammals different susceptibility virus. Models formed other created...
The aim was to identify conjunctival bacterial and fungal flora in clinically normal sheep.Prospective study.Tuscany.100 eyes from 50 adult Massese female sheep were examined. included the study considered free of anterior ophthalmic abnormalities.Bacteria identified by morphological assessment, Gram staining, biochemical tests. Identification filamentous fungi achieved at genus level, Aspergillus species based on keys provided other authors. Yeast colonies highlighted, but not...
The maedi-visna virus (MVV) is distributed worldwide and, to date, no epidemiological surveys have been carried out using ELISA testing in Turkey. aims of this study were as follows: i) verify the diagnostic performance a home-made test (HM-ELISA) Turkish ovine sera comparison with other commercially available tests, and ii) perform preliminary survey İstanbul province. MVV seroprevalence province was 15.3%. Older sheep 5 times more likely be positive than younger animals, ewes 3 rams, all...
Small Ruminant Lentiviruses (SRLVs) are widespread in many countries and cause economically relevant, slow, persistent diseases sheep goats. Monitoring the genetic diversity of SRLVs is useful to improve diagnostic tools used eradication programs. In this study, detected Spanish Assaf with different grades lymphoproliferative mastitis were sequenced. Genetic characterization showed that most samples belonged type A closer SRLV isolates previously classified as A2/A3. Four subtype B2 higher...
In order to characterize the complete range of lesions, especially minimal, affecting mammary gland and viral antigen distribution target cells using immunohistochemistry in naturally Visna/maedi (VM) 84 infected sheep were studied, forty-four from flocks with clinical cases (A) 35 randomly sampled two abattoirs (B) together five negative controls (C). An immunocytochemistry technique was developed further milk samples (n = 39) used study excretion, carrier role colostrum transmission...
Ocular infections due to Streptococcus dysgalactiae subsp. equisimilis are rare. In the present report, three patients with a history of uncomplicated small incision cataract surgery intraocular lens implantation developed exogenous endophthalmitis equisimilis. The identification organisms was confirmed by PCR for 16S rRNA sequence specific species S. dysgalactiae. Intravitreal treatment cefazolin and amikacin, in addition topical ofloxacin tobramycin, resulted resolution infection all...