A5061033912- Microbial infections and disease research
- Bacterial Identification and Susceptibility Testing
- Streptococcal Infections and Treatments
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Veterinary medicine and infectious diseases
- Veterinary Equine Medical Research
- Bacteriophages and microbial interactions
- Antibiotics Pharmacokinetics and Efficacy
- Infectious Disease Case Reports and Treatments
- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Ocular Infections and Treatments
- Mycobacterium research and diagnosis
- Antimicrobial Resistance in Staphylococcus
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Herpesvirus Infections and Treatments
- Infective Endocarditis Diagnosis and Management
- Nail Diseases and Treatments
- Gastrointestinal motility and disorders
- Animal Virus Infections Studies
- Ocular Surface and Contact Lens
- Burkholderia infections and melioidosis
- Infections and bacterial resistance
Japan Racing Association
2015-2025
Osaka International University
2023-2024
Osaka University
2023-2024
Ministry of Agriculture, Forestry and Fisheries
2019
Kagoshima University
2004-2016
Hidaka Hospital
2016
Centers for Disease Control and Prevention
2011
The University of Tokyo
2001-2011
Although many genes related to the pathogenicity of Campylobacter jejuni have been reported, relationships between these and sources strains are not clear. In this study, presence 11 pathogenic responsible for expression adherence, invasion, colonization cytotoxin production was examined in 111 C. isolated from human clinical samples, poultry meat, broiler faeces bovine faeces. For most genes, no difference their found among sources, but, racR, wlaN virB11, there were some variations...
On August 2007, we encountered equine influenza epidemic by Florida sub-lineage strain (H3N8) in Japan Racing Association's facilities where 4142 racehorses total were stabled. The number of new febrile cases sharply increased, but the occurrence was rapidly calmed down within 2 weeks. morbidity rate these 12.8% and subclinical infection healthy examined rapid antigen detection tests 19.4% at early stage epidemic. serological studies along with low existence numbers asymptomatically infected...
Recently, outbreaks associated with equine coronavirus (ECoV) have occurred in Japan and the United States. While ECoV is likely to be pathogenic horses, it has not been shown that experimental inoculation of horses produces clinical signs disease. In this study, we inoculated three Japanese draft an ECoV-positive diarrheic fecal sample confirm infection after investigate course virus shedding patterns ECoV. Virus neutralization tests showed all became infected Two developed similar those...
Bacterial keratitis of the horse is mainly caused by staphylococci, streptococci, and pseudomonads. Of these bacteria, Pseudomonas aeruginosa sometimes causes rapid corneal corruption and, in some cases, blindness. Antimicrobial resistance can make treatment very difficult. Therefore, new strategies to control bacterial infection are required. A bacteriophage (phage) a virus that specifically infects kills bacteria. Since phage often lyse antibiotic-resistant bacteria because killing...
Abstract Microbial communities are commonly studied by using amplicon sequencing of part the 16S rRNA gene. Sequencing full-length gene can provide higher taxonomic resolution and accuracy. To obtain even resolution, with as few false-positives possible, we assessed a method long targeting operon combined CCMetagen pipeline. Taxonomic assignment had > 90% accuracy at species level in mock sample family equine fecal samples, generating similar composition shotgun sequencing. The samples...
Glanders is a World Organization for Animal Health (WOAH)-notifiable equine disease caused by the infection of Burkholderia mallei, and endemic in Mongolia, South Asia, Africa, America. While complement fixation test (CFT) has been widely used serodiagnosis glanders considered standard serological test, it several limitations. These limitations include poor specificity, labor intensive techniques, variability antigen protocol. Consequently, indirect enzyme-linked immunosorbent assays...
In the present study, concentrations of serum amyloid A and surfactant protein D in sera were measured to evaluate them for identification clinical condition horses with bacterial pneumonia. The study utilized 185 clinically healthy control thoroughbreds 9 experimental infectious S. zooepidemicus. Blood samples collected from thoroughbreds. experimentally infected zooepidemicus using an endoscopic injection a lung lobe then observed conditions. before inoculation on 1-15th, 22nd, 29th days...
To describe the number of cases, etiologies, healing times, and results microbial culture susceptibility testing corneal ulcers in thoroughbred racehorses Japan.Retrospective study medical records belonging to Japan Racing Association (JRA) from 1997 2008.A total 2846 horses suffered ulcerative keratitis. These accounted for 90.5% all problems 54.9% entire with ocular diseases. Running races was cause 64.3% cases. The mean time developed 5.5 + or - 9.6 days, which shorter than that unrelated...
To describe the incidence, clinical progress, visual outcome, and laboratory findings of equine keratomycosis in Japan. Retrospective study medical records horses clinically mycologically diagnosed with at Equine Hospitals Japan Racing Association from 2005 to 2011.The diagnosis was confirmed eight (40.0% 20 infectious keratitis which fungi and/or bacteria were isolated). Fungi recovered corneal swabs identified as Aspergillus flavus (4), niger (1), Fusarium solani Mortierella wolfii (2)....
The equine disease strangles, which is characterized by the formation of abscesses in lymph nodes head and neck, one most frequently diagnosed infectious diseases horses around world. causal agent, Streptococcus equi subspecies equi, establishes a persistent infection approximately 10 % animals that recover from acute disease. Such 'carrier' appear healthy are rarely identified during routine veterinary examinations pre-purchase or transit, but can transmit S. to naïve initiating new...
Introduction Protection of horse welfare during transport is crucial. The aim this study was to determine the effect head and neck restraint on behavior airway bacteria. Methods In a randomized crossover study, six healthy Thoroughbreds were transported by road for 22 h in an individual bay with tight (50 cm short-rope) or loose (95 long-rope). Behavioral parameters relating position, eating, stress monitored transportation. Tracheal wash samples obtained 6 days before immediately after...
Clostridium difficile is an important cause of acute enterocolitis in horses. We describe five cases C infection occurring postoperatively Thoroughbred racehorses. Following diarrhoea or colic accompanied by a marked increase packed cell volume (to ≥60 per cent) and leucopenia (≤4000 cells/μl) within two to four days after surgery all horses, them died were euthanased because colitis severe diarrhoea. In these necrotising entero‐typhlo‐colitis was revealed postmortem examination, recovered...
MRSA is a known pathogen that affects horses. We investigated an equine isolate for potential antimicrobial resistance genes, classified the staphylococcal cassette chromosome mec (SCCmec) and identified strain-specific dissemination in horse community based on WGS.WGS, using short-read sequencing, subsequent long-read sequencing by hybrid assembly, was conducted to obtain complete genome sequence. Pairwise sequence alignment of relative SCCmec sequences core-genome phylogenetic analysis...
Background Although Clostridioides difficile ‐associated diseases (CDAD) is considered to be associated with colitis in horses, few studies have been performed a focus on the characteristics of CDAD thoroughbred racehorses. Methods Between 2010 and 2018, test for C. was using faecal samples from 137 racehorses presenting diarrhoea fever. The mortality rate, clinical findings, predisposing factors selected treatments were investigated retrospective manner. Results Twenty‐four cases diagnosed...
Since equine influenza A virus (H3N8) was transmitted to dogs in the United States 2004, causative virus, which is called canine (CIV), has become widespread dogs. To date, it remained unclear whether or not CIV-infected could transmit CIV horses. address this, we tested close contact between horses and experimentally infected with would result its interspecies transmission.Three pairs of animals consisting a dog inoculated (10(8.3) egg infectious dose 50/dog) healthy horse were kept...
We studied a recent epizootic of Getah virus infection among pigs in the southern part Ibaraki Prefecture and northern Chiba Prefecture, Japan, focusing on its possible association with outbreaks racehorses 2014 2015. The genomic sequence strain from an infected pig was analyzed to evaluate degree identity strains horses.Sera were collected September December 2012 2015 south (380 29 batches), 2010 north (538 104 batches). They examined by using virus-neutralizing test for virus....
Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) is a dominant pathogenic bacterium in equine pneumonia. We developed specific loop-mediated isothermal amplification (LAMP) method, which targets the gene encoding sorbitol-6-phosphate 2-dehydrogenase (sorD), for detecting S. and examined clinical efficacies of its use combination with each 3 DNA extraction methods easily used by veterinary practitioners, namely Loopamp PURE Extraction Kit, InstaGene Matrix conventional boiling...
Meticillin-resistant Staphylococcus aureus (MRSA) infections have been confirmed in hospitalised Thoroughbred racehorses at the hospitals of two training centres Japan since 2009. To investigate source infection, authors examined rate nasal MRSA colonisation 600 healthy racehorses, 53 veterinarians and 16 office staff racehorse centres. was not isolated from or hospital staff. However, (30.1 per cent), significantly higher than same hospitals. Also, 10 strains (62.5 cent) were classified as...