A5019402081- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Microbial infections and disease research
- Infective Endocarditis Diagnosis and Management
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Biochemical and Structural Characterization
- Diphtheria, Corynebacterium, and Tetanus
- Genomics and Phylogenetic Studies
- Rabies epidemiology and control
- Respiratory viral infections research
- Infections and bacterial resistance
- Virology and Viral Diseases
- Poxvirus research and outbreaks
- Immunodeficiency and Autoimmune Disorders
- Lipoproteins and Cardiovascular Health
- Plant Disease Resistance and Genetics
- vaccines and immunoinformatics approaches
- Animal Disease Management and Epidemiology
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Immunology Research
- Infectious Disease Case Reports and Treatments
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
University of Suffolk
2025
Erasmus MC
2021-2023
Animal Health Trust
2009-2021
Erasmus University Rotterdam
2021
Royal Veterinary College
2010
University of Pennsylvania
2008
Carter Center
2008
University of Virginia
2008
North Central Cancer Treatment Group
2008
Mayo Clinic
2008
The continued evolution of bacterial pathogens has major implications for both human and animal disease, but the exchange genetic material between host-restricted is rarely considered. Streptococcus equi subspecies (S. equi) a pathogen horses that evolved from zoonotic zooepidemicus zooepidemicus). These share approximately 80% genome sequence identity with important pyogenes. We sequenced compared genomes S. 4047 H70 screened strains around world to uncover evidence events have shaped led...
Strangles, the most frequently diagnosed infectious disease of horses worldwide, is caused by Streptococcus equi . Despite its prevalence, global diversity and mechanisms underlying evolution S. as a host-restricted pathogen remain poorly understood. Here, we define population structure this important reveal replacement in late 19th or early 20th Century. Our data dynamic genome that continues to mutate decay, but also amplify acquire genes despite organism having lost natural competence...
This is, to the best of our knowledge, first case report describe apparent transmission Streptococcus equi subsp. zooepidemicus from an infected dog a handler who subsequently developed severe systemic infection. Characterization haemolytic streptococci isolated both patient and dog, by phenotypic molecular analysis, confirmed canine human isolates were identical.
The acquisition of superantigen-encoding genes by Streptococcus pyogenes has been associated with increased morbidity and mortality in humans, the gain four superantigens equi is linked to evolution this host-restricted pathogen from an ancestral strain opportunistic subsp. zooepidemicus. A recent study determined that culture supernatants several S. zooepidemicus strains possessed mitogenic activity but lacked known genes. Here, we report identification activities three novel products szeF,...
The detection of anti-Streptococcus equi antibodies in the blood serum horses can assist with identification apparently healthy persistently infected carriers and prevention strangles outbreaks. aim current study was to use genome sequencing data develop an indirect enzyme linked immunosorbent assay (iELISA) that targets two S. equi-specific protein fragments. sensitivity specificity antigen A C iELISAs were compared SeM-based iELISA marketed by IDvet - diagnostic Vétérinaire (IDvet)....
Genome sequencing data for Streptococcus equi subspecies and zooepidemicus were used to develop a novel diagnostic triplex quantitative PCR (qPCR) assay targeting two genes specific S. (eqbE SEQ2190) unique 100 base pair control DNA sequence (SZIC) inserted into the SZO07770 pseudogene of strain H70. This strangles qPCR can provide results within 2 h sample receipt, has an overall sensitivity 93.9% specificity 96.6% relative eqbE singlex detects at levels below threshold culture assay, even...
Summary In this study, we determined the function of a novel non‐ribosomal peptide synthetase (NRPS) system carried by streptococcal integrative conjugative element (ICE), ICE Se2 . The NRPS shares similarity with yersiniabactin found in high‐pathogenicity island Yersinia sp. and is first its kind to be identified streptococci. We named product ‘equibactin’ genes locus eqbA–N. , although absolutely conserved Streptococcus equi causative agent equine strangles, was absent from all strains...
ABSTRACT Streptococcus equi is the causative agent of strangles, most frequently diagnosed infectious disease horses worldwide. The characterized by abscessation and swelling lymph nodes head neck, which can literally strangle horse to death. S. produces four recently acquired phage-associated bacterial superantigens (sAgs; SeeH, SeeI, SeeL, SeeM) that share homology with mitogenic toxins pyogenes . aim this study was characterize contribution each these sAgs activity in vitro quantify...
Reasons for performing study: Strangles is the most commonly diagnosed and important infectious disease of horses worldwide. Very little known about temporo-spatial molecular epidemiology strangles. The not notifiable in UK there are few published data on geographical locations outbreaks. Objective: To investigate whether typing a surface protein (SeM) Streptococcus equi ssp. (S. equi), causative agent strangles, useful epidemiological tool. Methods: variable region SeM gene was amplified...
The equine disease strangles, which is characterized by the formation of abscesses in lymph nodes head and neck, one most frequently diagnosed infectious diseases horses around world. causal agent, Streptococcus equi subspecies equi, establishes a persistent infection approximately 10 % animals that recover from acute disease. Such 'carrier' appear healthy are rarely identified during routine veterinary examinations pre-purchase or transit, but can transmit S. to naïve initiating new...
AIMS: To estimate the prevalence of β-haemolytic Lancefield group C streptococci in healthy dogs, cats and horses; to determine if frequent contact with horses was associated isolation these species from dogs cats; characterise recovered S. equi subsp. zooepidemicus isolates by multilocus sequence typing.METHODS: Oropharyngeal swabs were collected 197 72 cats, nasopharyngeal 93 horses. Sampling carried out at Massey University Veterinary Teaching Hospital, on sheep beef farms or premises...
Iceland is free of the major infectious diseases horses. However, in 2010 an epidemic respiratory disease unknown cause spread through country's native horse population 77,000. Microbiological investigations ruled out known viral agents but identified opportunistic pathogen Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) diseased animals. We sequenced genomes 257 isolates S. to differentiate from endemic strains. found that although multiple clones were present, one particular...
Streptococcus equi ssp. causes characteristic clinical signs that are most severe in young horses, including fever, purulent nasal discharge, and lymph node abscessation the head region.Clinical, serologic, microbiologic factors related to unexpectedly mild disease severity a natural outbreak of strangles immunologically naïve weanlings were investigated.One-hundred twelve warmblood weanlings.Prospective longitudinal observational study strangles. The entire cohort was examined at peak by...
The purpose of this cross-sectional study was to determine the seroprevalence Streptococcus equi in Israel, monitor seropositive horses over time and identify archived strains that were recovered from Israeli horses. A serological survey 200 healthy on 20 farms throughout Israel performed detect recent exposure S antigens C via indirect ELISA. Seroprevalence 9.5 per cent (19/200) positive found 30 (6/20) farms. Sixteen returned a serology result retested three six months later. Most (12/16)...
The Gram-positive bacterium Streptococcus equi subspecies (S. equi) is the causative agent of strangles, among most frequently diagnosed infectious diseases horses worldwide. Genome analysis S. strain 4047 (Se4047) identified a putative operon, Fim1, with similarity to pilus loci other bacteria. Fim1 locus was present in all strains and its close relative zooepidemicus zooepidemicus) that have been studied date. In this study we provide evidence structural proteins, SEQ_0936 CNE, are...
Phenotypic heterogeneity within a population of bacteria, through genetic or transcriptional variation, enables survival and persistence in challenging changing environments. We report here that recent clinical isolate S. equi, strain 1691 (Se1691), yielded mixture reduced capsule mucoid colonies on primary isolation when grown colistin-oxolinic acid blood agar (COBA) streptococcal selective plates. Passaging Se1691, with phenotype maintained this mixed phenotype. In contrast, passaging...
ABSTRACT Strangles, the most frequently diagnosed infectious disease of horses worldwide, is caused by Streptococcus equi . Despite its prevalence, global diversity and mechanisms underlying evolution S. as a host-restricted pathogen remain poorly understood. Here we define population structure this important reveal replacement in late 19 th or early 20 century, contemporaneous with spate conflicts. Our data dynamic genome that continues to mutate decay, but also amplify acquire genes...
Opportunistic pathogens must adapt to and survive in a wide range of complex ecosystems. Streptococcus zooepidemicus is an opportunistic pathogen horses many other animals, including humans. The assembly different surface architecture phenotypes from one genotype likely be crucial the successful exploitation such lifestyle. Construction series mutants revealed that serine recombinase, PinR, inverts 114 bp promoter SZO_08560, which bordered by GTAGACTTTA TAAAGTCTAC inverted repeats. Inversion...
ABSTRACT The native horse population of Iceland has remained free major infectious diseases. Between May and July 2010 an epidemic respiratory disease swept through the population. Initial microbiological investigations ruled out known equine viral agents as cause infections, but identified opportunistic pathogen Streptococcus zooepidemicus being frequently isolated from diseased animals. This diverse bacterial species a broad host range is usually regarded commensal horses. By genome...