A5101462778- Effects and risks of endocrine disrupting chemicals
- Pharmacogenetics and Drug Metabolism
- Neurosurgical Procedures and Complications
- Traumatic Brain Injury and Neurovascular Disturbances
- Hormonal and reproductive studies
- Intracranial Aneurysms: Treatment and Complications
- Estrogen and related hormone effects
- Drug Transport and Resistance Mechanisms
- Carcinogens and Genotoxicity Assessment
- Ruminant Nutrition and Digestive Physiology
- Spinal Fractures and Fixation Techniques
- Toxic Organic Pollutants Impact
- Vascular Malformations Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Microbial infections and disease research
- Cerebrospinal fluid and hydrocephalus
- Genetic and phenotypic traits in livestock
- Alzheimer's disease research and treatments
- Intracerebral and Subarachnoid Hemorrhage Research
- Reproductive Physiology in Livestock
- Bacteriophages and microbial interactions
- Spinal Hematomas and Complications
- Antimicrobial Peptides and Activities
- Cerebral Venous Sinus Thrombosis
- Drug-Induced Hepatotoxicity and Protection
Osaka Minami Medical Center
2001-2024
Nara Medical University
2004-2024
Nara Prefecture General Medical Center
2024
Rakuno Gakuen University
2013-2022
Osaka City General Hospital
2019-2022
Osaka Prefectural Medical Center
2019-2022
Osaka Police Hospital
2013-2022
Nara City Hospital
2011-2019
Higashi Osaka City General Hospital
1988-2019
BioChem Technology (United States)
2016
BackgroundBisphenol A (BPA), a well-known endocrine disruptor, is highly glucuronidated in the liver, and resultant BPA-glucuronide (BPA-GA) excreted primarily into bile. However, rodents, prenatal exposure to low doses of BPA can adversely affect fetus, despite efficient drug-metabolizing systems dams. The transport mechanisms from mother fetus are unknown.ObjectivesTo test our hypothesis that BPA-GA—an inactive metabolite—is passed through placenta where it affects after reactivation, we...
Bisphenol A, an environmental oestrogenic chemical, was found to conjugate highly with glucuronic acid in male rat liver microsomes studied vitro. In the various isoforms tested (1A1, 1A3, 1A5, 1A6, 1A7 and 2B1), glucuronidation of bisphenol A diethylstilboestrol, a synthetic crystalline compound possessing activity known be glucuronidated by microsomes, catalysed isoform UDP-glucuronosyltransferase (UGT), namely UGT2B1, which glucuronidates some endogenous androgens. UGT towards UGT2B1...
Xenoestrogens, such as bisphenol A and diethylstilbestrol, are glucuronidated by an isoform of UDP-glucuronosyltransferase named UGT2B1 in the livers adult male rats. In this study, we found that nonylphenol octylphenol also conjugated with glucuronic acid rat liver microsomes. Although activities toward these xenoestrogens were not detected fetal liver, a linear increase enzymatic during neonatal development was observed. At 3 weeks after birth, had reached same level The protein mRNA...
Bovine mastitis is an inflammation of the mammary gland caused by bacterial infection in dairy cattle. It most costly disease industry because high use antibiotics. Staphylococcus aureus one major causative agents bovine and antimicrobial resistance. Therefore, new strategies to control are required industry. One potential strategy bacteriophage (phage) therapy. In present study, we examined host range previously isolated S. phages ΦSA012 ΦSA039 against strains from mastitic cows. These...
To clarify the relationship between tumor necrosis factor (TNF) and insulin resistance in dairy cows affected with fatty liver, naturally occurring cases were investigated. The classified into following three groups according to histopathologic findings of liver: mild fat droplet deposition (group 1; n=11), severe 2; n=10), cloudy swelling 3; n=8). Serum TNF activities Group 2 (8.67 ± 2.16 U/ml) 3 (11.65 1.92 significantly higher than that 1 (3.57 0.81 (p<0.05). insulin-tolerance tests...
Despite the importance of short-chain fatty acids (SCFA) in maintaining ruminant physiology, mechanism SCFA absorption is still not fully studied. The goal this study was to elucidate possible involvement monocarboxylate transporter 1 (MCT1) transport caprine rumen, and delineate precise cellular localization level MCT1 protein along entire gastrointestinal tract. RT-PCR revealed presence mRNA encoding for all regions Quantitative Western blot analysis showed that order rumen >/= reticulum >...
Bisphenol A (BPA), a well-known endocrine disruptor, is metabolized and eliminated rapidly from the body in adult animals. However, many authors have reported that perinatal BPA exposure alters development of brain, reproductive system behavior next generation. Recently, substitutes, especially bisphenol F (BPF), been used because concerns about influence on children, although actual effects generation are unknown. In this study, we observed behavioral adverse offspring mice exposed to or...
Bacterial keratitis of the horse is mainly caused by staphylococci, streptococci, and pseudomonads. Of these bacteria, Pseudomonas aeruginosa sometimes causes rapid corneal corruption and, in some cases, blindness. Antimicrobial resistance can make treatment very difficult. Therefore, new strategies to control bacterial infection are required. A bacteriophage (phage) a virus that specifically infects kills bacteria. Since phage often lyse antibiotic-resistant bacteria because killing...
Bisphenol A, an environmental estrogen, can be leached from plastic tableware and the coating of food drink cans, orally exposing human beings to compound. The present study focuses on absorption metabolism bisphenol A in rat intestine, as elucidated experimentally by segmented everted intestine. One hour after application 2 μmol mucosal fluid, was slightly greater colon (48.6%) than proximal jejunum (37.5%). In serosal side, unconjugated appeared small amounts, increasing distally (maximal...
In male rats challenged with the environmental estrogen bisphenol A, compound is highly glucuronidated in liver and excreted largely into bile. Given that pregnancy microsomal glucuronidation toward A attenuated, we hypothesized elimination of from may be reduced pregnancy. This study was conducted to trace female rats, especially Sprague-Dawley 1.5 μmol perfused via portal vein. both nonpregnant female, infused glucuronidated, then resultant glucuronide mainly pregnant however, bilious...
Sivelestat sodium hydrate is a selective inhibitor of neutrophil elastase (NE), and effective in acute lung injury associated with systemic inflammatory response syndrome (SIRS). The effect for postoperative clinical courses after transthoracic esophagectomy was investigated. Consecutive patients carcinoma the thoracic esophagus who underwent between 2003 2004 were assigned to Sivelestat-treated group (n = 18), those 1998 control 25). morbidity rate, duration SIRS, mechanical ventilation,...
Sivelestat sodium hydrate is a selective inhibitor of neutrophil elastase, which effective in acute lung injury associated with systemic inflammatory response syndrome. However, the effectiveness sivelestat sepsis has not been fully examined. In present study, effect on severe rat cecal ligation and puncture (CLP) model was investigated. Adult male Sprague-Dawley rats underwent CLP were randomly divided into two groups: sivelestat-treated group saline-treated controls. The serum...
Flavonoids have poor bioavailabilities largely because of metabolism via UDP-glucuronosyltransferases (UGTs). This study aims to further understand the functions UGT in metabolizing genistein and apigenin, two compounds metabolized more extensively gut than liver. Because Gunn rats are deficient UGT1As, we determined whether this deficiency would result less flavonoid glucuronidation, using rat intestinal perfusion model microsomes prepared from liver intestine. In yeast-expressed isoforms,...
The actual levels of steroid hormones in organs are vital for endocrine, reproductive and neuronal health disorders. We developed an accurate method to determine the conjugates various by efficient preparation using a solid-phase-extraction cartridge. Each was identified precursor ion spectra liquid chromatography–electrospray ionization time-of-flight mass spectrometry, respective steroids were quantitatively analysed selected reaction monitoring mode chromatograph-mass spectrometry/mass...
Bisphenol A, an environmental oestrogenic chemical, was found to conjugate highly with glucuronic acid in male rat liver microsomes studied vitro. In the various isoforms tested (1A1, 1A3, 1A5, 1A6, 1A7 and 2B1), glucuronidation of bisphenol A diethylstilboestrol, a synthetic crystalline compound possessing activity known be glucuronidated by microsomes, catalysed isoform UDP-glucuronosyltransferase (UGT), namely UGT2B1, which glucuronidates some endogenous androgens. UGT towards UGT2B1...
Brain-specific angiogenesis inhibitor 2 (BAI2) is a transmembrane protein that predominantly expressed in the brain. Although BAI2 supposed to correlate with antiangiogenesis brain, its psychiatric function still unclear. In this study, we examined influence of gene disruption on mood-related behavior using BAI2-deficient mice. mice showed significant antidepressant-like social defeat test and tail suspension compared wild-type On other hand, had normal basal locomotor activity home cage...
Summary Blood chemical values, including ketone bodies, were measured in 25 cows with abomasal displacement (displacement group), 16 primary ketosis (ketosis and nine normal controls to investigate the pathophysiology of displacement. Increases aspartate aminotransferase, γ‐glutamyl transpeptidase, non‐esterified fatty acid (NEFA), bodies (3‐hydroxybutyric acetoacetic acid) observed groups. Total cholesterol increased significantly group but decreased group. Glucose was low reversely...
A simplified polymerase chain reaction (PCR) assay was developed for fast and easy screening of mycoplasma mastitis in dairy cattle.Species major strains [Mycoplasma (M.) bovis, M. arginini, bovigenitalium, californicum, bovirhinis, alkalescens canadense] cultured milk samples were detected by this PCR-based method as well a standard PCR technique.The minimum concentration limit detecting the estimated to be about 2.5 × 10 3 cfu/mL similar that PCR.We compared specificity sensitivity those...