A5020839989- Metal-Catalyzed Oxygenation Mechanisms
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Porphyrin and Phthalocyanine Chemistry
- Hemoglobin structure and function
- Metal complexes synthesis and properties
- Magnetism in coordination complexes
- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Porphyrin Metabolism and Disorders
- Evolution and Genetic Dynamics
- Metalloenzymes and iron-sulfur proteins
- Heme Oxygenase-1 and Carbon Monoxide
- Electrocatalysts for Energy Conversion
- Crystallography and molecular interactions
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Immunotherapy and Immune Responses
- Block Copolymer Self-Assembly
- Electrochemical sensors and biosensors
- Rheology and Fluid Dynamics Studies
- Mass Spectrometry Techniques and Applications
- Material Dynamics and Properties
- Cancer, Lipids, and Metabolism
University of Hyogo
2005-2022
Sanyo-Onoda City University
2019-2020
Hyogo University
2015-2019
Kyushu University
1984-2016
Fukuoka University
2015-2016
Aichi Institute of Technology
2015
National Institute of Genetics
1967-2013
Japan Science and Technology Agency
2009-2013
Nara Institute of Science and Technology
2010
Doshisha University
2010
A mathematical theory is developed that enables us to derive a formula for the equilibrium distribution of allelic frequencies in finite population when selectively neutral alleles are produced stepwise fashion (stepwise mutation model). It shown model has remarkable property distinguishes it from conventional infinite allele (Kimura-Crow model): as size increases indefinitely while product effective and rate kept at fixed value, mean number different contained rapidly reaches plateau which...
Abstract Terminal cobalt(IV)–oxo (Co IV –O) species have been implicated as key intermediates in various cobalt-mediated oxidation reactions. Herein we report the photocatalytic generation of a mononuclear non-haem [(13-TMC)Co (O)] 2+ ( 2 ) by irradiating [Co II (13-TMC)(CF 3 SO )] + 1 presence [Ru (bpy) ] , Na S O 8 and water an oxygen source. The intermediate was also obtained reacting with artificial oxidant (that is, iodosylbenzene) characterized spectroscopic techniques. In particular,...
The reactions of manganese(III) porphyrin complexes with terminal oxidants, such as m-chloroperbenzoic acid, iodosylarenes, and H(2)O(2), produced high-valent manganese(V)-oxo porphyrins in the presence base organic solvents at room temperature. have been characterized various spectroscopic techniques, including UV-vis, EPR, 1H 19F NMR, resonance Raman, X-ray absorption spectroscopy. combined results indicate that are diamagnetic low-spin (S = 0) species a longer, weaker Mn-O bond than...
Host-encoded factors play an important role in virus multiplication, acting concert with virus-encoded factors. However, information regarding the host involved this process is limited. Here we report map-based cloning of Arabidopsis thaliana gene, TOM1 , which necessary for efficient multiplication tobamoviruses, positive-strand RNA viruses infecting a wide variety plants. The mRNA suggested to encode 291-aa polypeptide that predicted be multipass transmembrane protein. Sos recruitment...
A series of complexes [Fe(IV)(O)(TMC)(X)](+) (where X = OH(-), CF3CO2(-), N3(-), NCS(-), NCO(-), and CN(-)) were obtained by treatment the well-characterized nonheme oxoiron(IV) complex [Fe(IV)(O)(TMC)(NCMe)](2+) (TMC tetramethylcyclam) with appropriate NR4X salts. Because topology TMC macrocycle, represents an extensive collection S 1 that only differ respect to ligand trans oxo unit. Electronic absorption, Fe K-edge X-ray resonance Raman, Mossbauer data collected for these conclusively...
The gene encoding D-lactate dehydrogenase (D-lactate: NAD+ oxidoreductase, EC 1.1.1.28) of Lactobacillus plantarum has been sequenced, and expressed in Escherichia coli cells with an inducible expression plasmid, which the 5'-noncoding region was replaced tac promoter.Comparison sequence D-laCtate L-lactate dehydrogenases, including L. dehydrogenase, showed no significant homology.In contrast, is homologous to E. D-3-phosphoglycerate casei D-2-hydroxyisocaproate dehydrogenase.This indicates...
Penicillin-binding protein (PBP)-2 and the RodA are known to function in determining rod shape of Escherichia coli cells.Peptidoglycan biosynthetic reactions that required these two proteins were demonstrated membrane fraction prepared from an E. strain overproduced both which lacked PBP-1B activity (the major peptidoglycan synthetase normal membranes).The cross-linked was synthesized UDP-N-acetylmuramylpentapeptide UDP-N-acetylglucosamine presence a high concentration cefmetazole inhibited...
Axial ligand substitution of a mononuclear nonheme oxoiron(IV) complex, [FeIV(O)(TMC)(NCCH3)]2+ (1) (TMC = 1,4,8,11-tetramethyl-1,4,8,11-tetraazacyclotetradecane), leads to the formation new FeIV=O species with relatively intense electronic absorption features in near-UV region. The presence these allowed us make first observation Fe=O vibrations S 1 complexes by resonance Raman spectroscopy. We have also demonstrated that reactivity intermediates is markedly influenced axial ligands.
Class A beta-lactamases are known to hydrolyze substrates through a Ser70-linked acyl-enzyme intermediate, although the detailed mechanism remains unknown. On basis of tertiary structure active site, role Glu166 class enzymes was investigated by replacing residue in RTEM-1 beta-lactamase with Ala, Asp, Gln, or Asn. All mutants, contrast wild-type, accumulated covalent complex benzylpenicillin which corresponds an intermediate. For Asp mutant, decayed slowly and hydrolytic activity slightly...
The evolution of multigene families whose members are dispersed into two or more nonhomologous chromosomes is studied from the standpoint population genetics. By using a simple model gene conversion, equilibrium and transient properties probability identity genes belonging to family investigated. Also, time until fixation mutant subdivided by an approximation method. It shown that dispersion has relatively minor effect on extent between genes, unless conversion rate extremely low, number which large.
Sites for Cys substitutions to form a disulfide bond were chosen in subtilisin E from Bacillus subtilis, cysteine-free bacterial serine protease, based on the structure of aqualysin I Thermus aquaticus YT-1 (a thermophilic subtilisin-type protease containing two bonds). residues introduced at positions 61 (wild-type, Gly) and 98 (Ser) by site-directed mutagenesis. The Cys-61/Cys-98 mutant appeared spontaneously expression system used showed catalytic efficiency equivalent that wild-type...
Abstract In order to clarify the nature of "near neutrality" in molecular evolution and polymorphism, extensive simulation studies were performed. Selection coefficients new mutations are assumed be small so that both random genetic drift selection contribute determining behavior mutants. The model also incorporates normally distributed spatial fluctuation coefficients. If system starts from "average neutrality," it will move a better adapted state, most become "slightly deleterious." Monte...
Aqualysin I is a subtilisin-type serine protease which secreted into the culture medium by Thermus aquaticus YT-1, an extremely thermophilic Gram-negative bacterium. The nucleotide sequence of entire gene for aqualysin was determined, and deduced amino acid suggests that produced as large precursor, consisting at least three portions, NH2-terminal pre-pro-sequence (127 residues), (281 COOH-terminal pro-sequence (105 residues). When cloned expressed in Escherichia coli cells, not secreted....
Modeling job: Protonation of a closed seven-coordinate side-on heme peroxide can switch its spin state from high- to low-spin and convert the η2 binding fashion into monodentate configuration afford corresponding hydroperoxide (see scheme). The end-on ferric hydroperoxo–heme species bear covalently appended axial imidazole ligand are easily prepared.
The equation of motion is derived for the interfaces formed in general reaction-diffusion system which includes systems undergoing phase transformations some strong coupling regime where interface thickness can be regarded as small compared to other length scales problem. Taking into account effects curvature permits extensions theory “interface” one spatial dimension described Appendix multi-dimensional cases. related work Langer and Turski discussed light present investigation.
A functional heme/nonheme nitric oxide reductase (NOR) model is presented. The fully reduced diiron compound reacts with two equivalents of NO leading to the formation one equivalent N(2)O and bis-ferric product. binds both heme Fe nonheme complexes forming individual ferrous nitrosyl species. mixed-valence species an oxidized a Fe(B) does not show reduction activity. These results are consistent so-called "trans" mechanism for by bacterial NOR.
With advancements in stem cell technology, vitro models using iPSC (induced pluripotent cells)-derived cardiomyocytes (iPSC-CM) and engineered heart tissues (EHT) can serve as powerful tools for disease modeling drug screening. ...Fluorescent reporters of cardiac electrophysiology provide valuable information on tissue function. However, motion artifacts caused by muscle contraction interfere with accurate measurement fluorescence signals. Although drugs ...
Abstract The FeMo-cofactor of nitrogenase, a metal–sulfur cluster that contains eight transition metals, promotes the conversion dinitrogen into ammonia when stored in protein. Although various clusters have been synthesized over past decades, their use activation N 2 has remained challenging, and even extracted from nitrogenase is not able to reduce . Herein, we report by molybdenum titanium. An moiety bridging two [Mo 3 S 4 Ti] cubes converted NH H upon treatment with Brønsted acids...
Mononuclear nonheme iron(V)-oxo complexes have been reported previously. Herein, we report the first example of a mononuclear iron(V)-imido complex bearing tetraamido macrocyclic ligand (TAML), [(TAML)FeV(NTs)]- (1). The spectroscopic characterization 1 revealed an S = 1/2 Fe(V) oxidation state, Fe-N bond length 1.65(4) Å, and vibration at 817 cm-1. reactivity was demonstrated in C-H functionalization nitrene transfer reactions.
Abstract Reaction of O 2 with a high‐spin mononuclear iron(II) complex supported by five‐azole donor set yields the corresponding non‐heme iron(III)–superoxo species, which was characterized UV/Vis spectroscopy and resonance Raman spectroscopy. 1 H NMR analysis reveals diamagnetic nature superoxo arising from antiferromagnetic coupling between spins on low‐spin iron(III) superoxide. This species reacts H‐atom donating reagents to give iron(III)–hydroperoxo showing characteristic UV/Vis,...
Activation of dioxygen (O
Reported here is the N2 cleavage of a one-electron oxidation reaction using trans-[Mo(depe)2 (N2 )2 ] (1) (depe=Et2 PCH2 CH2 PEt2 ), which classical molybdenum(0)-dinitrogen complex supported by two bidentate phosphine ligands. The molybdenum(IV) terminal nitride [Mo(depe)2 N][BArf4 (2) (BArf4 =B(3,5-(CF3 C6 H3 )4 ) synthesized 1 upon addition mild oxidant, [Cp2 Fe][BArf4 (Cp=C5 H5 and proceeds from MoII -N=N-MoII structure. In addition, electrochemical for also cleaved ligand to give 2....
The rodA(Sui) mutation allows cell division to take place at 42 degrees C in ftsI23 mutant cells, which produce a thermolabile penicillin-binding protein 3 (PBP3, the septation-specific peptidoglycan transpeptidase). We show here that rodA is single-base change from glutamine chain termination (amber) codon, and an amber suppressor (supE) present strain restores ability reduced level of normal RodA protein. accompanied by increase levels two other proteins (PBP2 PBP5) encoded genes operon....