A5061540772- Enzyme Structure and Function
- Enzyme Production and Characterization
- Amino Acid Enzymes and Metabolism
- Microbial Metabolites in Food Biotechnology
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Protein Structure and Dynamics
- Polysaccharides and Plant Cell Walls
- Metabolism and Genetic Disorders
- Cancer, Hypoxia, and Metabolism
- Biochemical and Molecular Research
- Glycosylation and Glycoproteins Research
- Diet, Metabolism, and Disease
- Heme Oxygenase-1 and Carbon Monoxide
- Biochemical Acid Research Studies
- Studies on Chitinases and Chitosanases
- Peptidase Inhibition and Analysis
- Carbohydrate Chemistry and Synthesis
- Enzyme Catalysis and Immobilization
- Plant-Microbe Interactions and Immunity
- Thermal Regulation in Medicine
- Semiconductor Quantum Structures and Devices
- Semiconductor materials and devices
- Immune Cell Function and Interaction
- Microbial metabolism and enzyme function
Tokyo University of Science
2015-2025
Fujifilm (Japan)
2019
The University of Tokyo
1982-1997
Toho University
1995
Tokyo University of Agriculture
1982-1994
Institute for Fermentation
1991
Tohoku University
1991
Friedrich Miescher Institute
1984-1987
Osaka University
1983
NEC (Japan)
1978
Two cDNA clones for rat heme oxygenase have been isolated from a spleen library in lambda gt11 by immunological screening using specific polyclonal antibody. One of these has an insert 1530 nucleotides that contains the entire protein-coding region. To confirm encodes oxygenase, we transfected monkey kidney cells (COS-7) with carried simian virus 40 vector. The was highly expressed endoplasmic reticulum cells. nucleotide sequence cloned determined and primary structure deduced. Heme is...
A heat shock element is located in the 5'-flanking region of rat heme oxygenase gene (HO gene). The incubation glioma cells at 42 degrees C or with hemin 37 increased levels mRNA within 1 h and produced a maximum 3 (at least 20-fold increase). In both treatments, activity started to increase after lag period about reached value 5 h. There was an apparent additive effect treatments on induction. Studies actinomycin D cycloheximide suggested that acted transcriptional level induce oxygenase....
Long-term senescent cells exhibit a secretome termed the senescence-associated secretory phenotype (SASP). Although mechanisms of SASP factor induction have been intensively studied, release mechanism and how factors influence tumorigenesis in biological context remain unclear. In this study, using mouse model obesity-induced hepatocellular carcinoma (HCC), we identified factors, which include interleukin-1β (IL-1β)- IL-1β-dependent IL-33, from hepatic stellate (HSCs) via gasdermin D (GSDMD)...
Heme oxygenase, an essential enzyme of heme catabolism, is inducible by its substrate heme, heavy metals, and various other substances. To study the molecular mechanisms induction we isolated oxygenase gene from a rat genomic DNA library using cloned cDNA as hybridization probes determined complete nucleotide sequence. The composed 6830 nucleotides, organized in four introns five exons. transcription initiation site was identified S1 nuclease mapping analysis. Using HeLa cell lysate,...
Escherichia coli TUH12191, which is resistant to piperacillin, cefazolin, cefotiam, ceftizoxime, cefuzonam, and aztreonam but susceptible cefoxitin, latamoxef, flomoxef, imipenem, was isolated from the urine of a patient treated with beta-lactam antibiotics. The beta-lactamase (Toho-1) purified bacteria had pI 7.8, molecular weight about 29,000, hydrolyzed antibiotics such as penicillin G, ampicillin, oxacillin, carbenicillin, cephalothin, cefotaxime, ceftazidime, aztreonam. Toho-1 markedly...
The gene encoding D-lactate dehydrogenase (D-lactate: NAD+ oxidoreductase, EC 1.1.1.28) of Lactobacillus plantarum has been sequenced, and expressed in Escherichia coli cells with an inducible expression plasmid, which the 5'-noncoding region was replaced tac promoter.Comparison sequence D-laCtate L-lactate dehydrogenases, including L. dehydrogenase, showed no significant homology.In contrast, is homologous to E. D-3-phosphoglycerate casei D-2-hydroxyisocaproate dehydrogenase.This indicates...
Thrombomodulin decreased by 20-30% the Michaelis constant of two tripeptidyl p-nitroanilide substrates thrombin. increased rate inactivation thrombin peptidyl chloromethane inhibitors a similar amount. This effect appeared to be due decrease in dissociation constants inhibitors. An improved method for separation fibrinopeptides A and B h.p.l.c. was developed, this used study thrombomodulin on thrombin-catalysed cleavage fibrinogen. In reaction, competitive inhibitor with respect alpha-chain...
The effects of structural properties and their changes during cellulose hydrolysis on the enzymatic rate have been studied from reaction mechanism point view. Important findings are following: (1) crystallinity index (CrI) partially crystalline increases as proceeds, a significant slowing down is, in large part, attributable to this change substrate. (2) completely disordered cellulose, like phosphoric-acid-treated does not significantly, relatively high is maintained hydrolysis. (3)...
Aqualysin I is an alkaline serine protease which secreted into the culture medium by Thermus aquaticus YT‐1. was purified, and its apparent relative molecular mass determined to be 28 500. The enzyme contained four Cys residues (probably as two cystines), amino acids composition similar those of cysteine‐containing proteases (proteinase K, etc.) well subtilisins. NH 2 ‐terminal sequence aqualysin showed homology with microbial proteases. optimum pH for proteolytic activity around 10.0. Ca 2+...
p94/calpain 3 is a skeletal muscle-specific Ca(2+)-regulated cysteine protease (calpain), and genetic loss of p94 activity causes muscular dystrophy (calpainopathy). In addition, small in-frame deletion in the N2A region connectin/titin that impairs p94-connectin interaction severe (mdm) mice. Since via its with M-line regions connectin becomes part filament system serves as molecular scaffold for myofibril, it has been proposed structural functional integrity complex essential health...
Abstract Cyclic β-1,2-glucan synthase (CGS) is a key enzyme in production of cyclic β-1,2-glucans (CβGs) which are involved bacterial infection or symbiosis to host organisms. Nevertheless, mechanism cyclization, the final step CGS reaction, has not been fully understood. Here we performed functional and structural analyses cyclization domain alone from Thermoanaerobacter italicus (TiCGS Cy ). We first found that β-glucosidase-resistant compounds produced by TiCGS with linear as substrates....
β-1,2-Glucan is an extracellular cyclic or linear polysaccharide from Gram-negative bacteria, with important roles in infection and symbiosis. Despite β-1,2-glucan's importance bacterial persistence pathogenesis, only a few reports exist on enzymes acting both β-1,2-glucan. To this end, we purified endo-β-1,2-glucanase to homogeneity cell extracts of the environmental species Chitinophaga arvensicola, candidate gene (Cpin_6279) was cloned related pinensis. The Cpin_6279 protein specifically...
Abstract Galactosides are major carbohydrates that found in plant cell walls and various prebiotic oligosaccharides. Studying the detailed biochemical functions of β-galactosidases degrading these is important. In particular, identifying with new substrate specificities could help production potentially beneficial this study, we identify a β-galactosidase novel specificity from Bacteroides xylanisolvens , an intestinal bacterium. The enzyme do not show hydrolytic activity toward natural...
4‐α‐Glucanotransferase was purified from cells of Thermococcus litoralis , a hyperthermophilic archaeon. The molecular mass the enzyme estimated to be approximately 87 kDa by gel filtration. optimal temperature for its activity 90 0 C. catalyzed transglycosylation maltooligosaccharides, yielding maltooligosaccharides various lengths and glucose. When maltoheptaose used as substrate, glucoamylase‐resistant glucoamylase‐sensitive saccharides were produced. On incubation amylose with T. enzyme,...
Journal Article Heat-Stable and Fructose 1,6-Bisphosphate-Activated L-Lactate Dehydrogenase from an Extremely Thermophilic Bacterium Get access Hayao TAGUCHI, TAGUCHI Department of Agricultural Chemistry, The University TokyoYayoi, Bunkyo-ku, Tokyo 113 Search for other works by this author on: Oxford Academic PubMed Google Scholar Minoru YAMASHITA, YAMASHITA Hiroshi MATSUZAWA, MATSUZAWA Takahisa OHTA Biochemistry, Volume 91, Issue 4, April 1982, Pages 1343–1348,...
We characterized recombinant Lin1839 protein (Lin1839r) belonging to glycoside hydrolase family 94 from Listeria innocua. Lin1839r catalyzed the synthesis of a series 1,2-β-oligoglucans (Sopn: n denotes degree polymerization) using sophorose (Sop2) as acceptor and α-d-glucose 1-phosphate (Glc1P) donor. recognized glucose very weak substrate form polymeric 1,2-β-glucan. The polymerization 1,2-β-glucan gradually decreased with long-term incubation generate Sopns. Kinetic analysis...
The rice blast fungus Magnaporthe oryzae differentiates a specialized infection structure called an appressorium to invade cells. In this report, we show that CBP1, which encodes chitin-deacetylase, is involved in the induction phase of differentiation. We demonstrate enzymatic activity Cbp1 critical for formation. M. has six CDA homologues addition Cbp1, but none these are indispensable observed chitosan localization at fungal cell wall using OGA488. This observation suggests Cbp1-catalysed...
The pigment cell-specific gene, located at the brown (b) locus in mouse, has been cloned and characterized. Its gene product is required for formation of black melanin rather than brown, although its exact function remains to be elucidated. We thus tentatively named it b-locus protein this report. about 18 kilobase pairs long organized into 8 exons 7 introns. Functional analysis promoter region suggests that nucleotide residues -38/154 sufficient direct transcription melanoma whole cell...
1,2-β-Glucan was produced enzymatically from 1.0 M sucrose and 0.5 glucose by the combination of phosphorylase 1,2-β-oligoglucan in presence 100 mM inorganic phosphate. Accumulation 1,2-β-glucan 2 L reaction mixture reached over 800 (glucose equivalent). Sucrose, fructose were removed after yeast treatment. precipitated with ethanol to obtain 167 g 1 mixture.
Despite the presence of β-1,2-glucan in nature, few degrading enzymes have been reported to date. Recently, Lin1839 protein from Listeria innocua was identified as a 1,2-β-oligoglucan phosphorylase. Since adjacent lin1840 gene cluster encodes putative glycoside hydrolase family 3 β-glucosidase, we hypothesized that Lin1840 is also involved dissimilation. Here report functional and structural analysis Lin1840. A recombinant (Lin1840r) showed highest hydrolytic activity toward sophorose...
Glycoside phosphorylases catalyze the phosphorolysis of oligosaccharides into sugar phosphates. Recently, we found a novel phosphorylase acting on β-1,2-glucooligosaccharides with degrees polymerization 3 or more (1,2-β-oligoglucan phosphorylase, SOGP) in glycoside hydrolase family (GH) 94. Here, characterized SOGP from Lachnoclostridium phytofermentans (LpSOGP) and determined its crystal structure. LpSOGP is monomeric enzyme that contains unique β-sandwich domain (Ndom1) at N-terminus....
BT _3567 protein, a putative β‐glucosidase from Bacteroides thetaiotaomicron , exhibits higher activity toward Sop 3–5 (Sop n n: degree of polymerization β‐1,2‐glucooligosaccharides) than 2 unlike known Listeria innocua which predominantly prefers . In the complex structure determined by soaking D286N mutant crystal with 4 3 moiety was observed at subsites −1 to +2. The glucose subsite +2 forms hydrogen bond Asn81, is replaced Gly in L. β‐glucosidase. K m values N81G for are much those...
Abstract Lactobacillus casei L ‐lactate dehydrogenase (LCLDH) is activated through the homotropic and heterotropic activation effects of pyruvate fructose 1,6‐bisphosphate (FBP), respectively, exhibits unusually high pH‐dependence in allosteric these ligands. The active (R) inactive (T) state structures unliganded LCLDH were determined at 2.5 2.6 Å resolution, respectively. In catalytic site, structural rearrangements are concerned mostly switching orientation Arg171 flexible intersubunit...
endo-β-1,2-Glucanase (SGL) is an enzyme that hydrolyzes β-1,2-glucans, which play important physiological roles in some bacteria as a cyclic form. To date, no eukaryotic SGL has been identified. We purified from Talaromyces funiculosus (TfSGL), soil fungus, to homogeneity and then cloned the complementary DNA encoding enzyme. TfSGL shows significant sequence similarity any known glycoside hydrolase (GH) families, but certain proteins with unknown functions. The recombinant (TfSGLr)...