A5075242662- Plant Virus Research Studies
- Fungal and yeast genetics research
- Plant and Fungal Interactions Research
- CRISPR and Genetic Engineering
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Forest Insect Ecology and Management
- Bacteriophages and microbial interactions
- X-ray Diffraction in Crystallography
- Advanced Chemical Physics Studies
- Enzyme Structure and Function
- DNA Repair Mechanisms
- Mesenchymal stem cell research
- Chromosomal and Genetic Variations
- Plant Genetic and Mutation Studies
- Mycorrhizal Fungi and Plant Interactions
- Insect symbiosis and bacterial influences
- Plant Disease Resistance and Genetics
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
The University of Tokyo
2024
Kikkoman (Japan)
2024
University of Tsukuba
2022-2023
Institute of Agrobiological Sciences
2021
National Agriculture and Food Research Organization
2021
Tokyo University of Science
2015-2019
Kobe University
2014
The rice blast fungus Magnaporthe oryzae differentiates a specialized infection structure called an appressorium to invade cells. In this report, we show that CBP1, which encodes chitin-deacetylase, is involved in the induction phase of differentiation. We demonstrate enzymatic activity Cbp1 critical for formation. M. has six CDA homologues addition Cbp1, but none these are indispensable observed chitosan localization at fungal cell wall using OGA488. This observation suggests Cbp1-catalysed...
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome editing has become a promising approach for efficient and versatile genetic engineering in various organisms; however, simple precise nucleotide modification methods filamentous fungi have been restricted to double crossover type homologous recombination (HR). In this study, we developed novel strategy via single crossover-mediated HR the model fungus Pyricularia...
Direct delivery of CRISPR/Cas9 ribonucleoproteins into the shoot apical meristem via particle bombardment enabled introduction a semidwarf1-orthologous mutation an elite wheat variety.
Mycoviruses are viruses that infect fungi. Unlike mammalian infectious viruses, their life cycle does not generally have an extracellular stage, and a symbiosis-like relationship is maintained between virus host Recently, mycoviruses been reported to show effects on fungi, altering biological properties such as growth rate, virulence, drug resistance, metabolite production. In this study, we systematically elucidated the of cells by comparing phenotypes transcriptomic responses in multiple...
RNA viruses in fungi (mycoviruses) are model systems for understanding the relationships between eukaryotic microorganisms and viruses. To reveal effects of mycoviruses on host fungi, it is essential to compare phenotypes isogenic fungal isolates with or without virus infection. Since active entry machinery has never been identified, introducing a difficult time-consuming process. Therefore, most studies have tried generate virus-free from infected strains by eliminating mycovirus. However,...
Abstract Chloramphenicol (Cm) is a broad-spectrum classic antibiotic active against prokaryotic organisms. However, Cm has severe side effects in eukaryotes of which the cause remains unknown. The plant pathogenic fungus Magnaporthe oryzae , causes rice blast, forms an appressorium to infect host cell via single-cell differentiation. specifically inhibits formation, indicates that novel molecular target (or targets) blast fungus. Application T7 phage display method inferred MoDullard,...
Metabolic switching and rewiring play a dynamic role in programmed cell differentiation. Many pathogenic microbes need to survive nutrient-deficient conditions use the glyoxylate cycle, an anaplerotic pathway of tricarboxylic acid produce carbohydrates. The plant fungus Magnaporthe oryzae (Pyricularia oryzae) has unique chitin deacetylase, Cbp1. spatiotemporal activity this protein is required for modification M. wall differentiation into specialized infection structure (appressorium). Here...
We report preliminary natural circular dichroism (NCD) spectra of amino acids, Land D-histidine, thin films in the nitrogen K-edge energy region. NCD peaks were observed at 399.9 eV and assigned to 1s → π* transition atoms imidazole ring. Imidazole ring is positioned side chain distant from asymmetric carbon. This result suggests that spectroscopy soft X-ray region can selectively pick up information on local structure around specific atoms, regardless position molecules. It interesting view...
Abstract The ascomycete Pyricularia oryzae (teleomorph: Magnaporthe oryzae) causes one of the most serious diseases known as rice blast. Nijmegen breakage syndrome protein (NBS1) is essential for DNA repair; thus, we studied P. NBS1 homolog (PoNBS1). A PoNBS1 null mutant exhibited high sensitivity to damage-inducing agents. also retarded hyphal growth, and induced abnormal conidial germination shape, but showed normal appressorium formation. phenotypes were complemented by introducing cDNA...
真菌には様々な寄生体が共存している.DNA/RNAウイルスやプラスミド,プリオンが知られており,中でもRNAウイルスは高頻度に見出されている.これら真菌を宿主とするRNAウイルス(以下マイコウイルス)の特筆すべき特徴は,宿主真菌細胞を死滅させることはなく,宿主細胞と共存状態を維持しているという点にある.このようなマイコウイルスは宿主真菌の菌糸成長や胞子形成,気中菌糸形成,色素沈着といったマクロな表現型に留まらず,酵素や二次代謝物の産生,種々のストレス耐性,遺伝子発現などあらゆる形質に影響を及ぼし得ることが明らかになっている.本稿では特にマイコウイルスがマイコトキシンをはじめとする真菌の二次代謝物の産生に及ぼす事例を紹介する.
Abstract In planta genome editing represents an attractive approach to engineering crops/varieties that are recalcitrant culture-based transformation methods. Here, we report the direct delivery of CRISPR/Cas9 ribonucleoproteins into shoot apical meristem using in particle bombardment and introduction a semidwarf1 (sd1) -orthologous mutation wheat. The triple knockout tasd1 mutant elite wheat variety reduced culm length by 10% without reduction yield.