A5090201638- Glycosylation and Glycoproteins Research
- Liver Disease Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Protein Kinase Regulation and GTPase Signaling
- Hepatitis C virus research
- Immune Cell Function and Interaction
- Receptor Mechanisms and Signaling
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Immunotherapy and Immune Responses
- Genetic and Kidney Cyst Diseases
- Cell Adhesion Molecules Research
- Acute Myeloid Leukemia Research
- S100 Proteins and Annexins
- Chemokine receptors and signaling
- Ion channel regulation and function
- Liver Disease and Transplantation
- Hepatitis B Virus Studies
- Erythrocyte Function and Pathophysiology
- Microtubule and mitosis dynamics
- Cancer, Hypoxia, and Metabolism
- Gastric Cancer Management and Outcomes
- HER2/EGFR in Cancer Research
- Gastrointestinal Tumor Research and Treatment
Nagoya City University
1982-2024
Teikyo University
2005-2024
Kashiwa Municipal Hospital
2015-2024
Fukushima Medical University Hospital
2023-2024
Fujita Health University Hospital
2024
Tohoku University
2014-2023
Mie University
2023
Nihon University Itabashi Hospital
2023
Osaka University
1992-2022
Mizuho (Japan)
1988-2022
The ganglioside GM3 synthase (SAT-I), encoded by a single-copy gene, is primary glycosyltransferase for the synthesis of complex gangliosides. In SAT-I null mice, hearing ability, assessed brainstem auditory-evoked potentials (BAEP), was impaired at onset and had been completely lost 17 days after birth (P17), showing deformity in hair cells organ Corti. By 2 months age, Corti selectively disappeared without effect on balance or motor function histology vestibule. Interestingly,...
Depolarization of the sarcolemma smooth muscle cells activates voltage-gated Ca2+ channels, influx and activation cross-bridge cycling by phosphorylation myosin catalysed Ca2+/calmodulin-dependent light-chain kinase (MLCK). Agonist stimulation contraction often involves other kinases in addition to MLCK. In present study, we address hypothesis that membrane depolarization-induced rat caudal arterial may involve Rho-associated (ROK). Addition 60mM K+ de-endothelialized strips presence...
Ganglioside GM3 is a major glycosphingolipid in the plasma membrane and widely distributed vertebrates. We describe here isolation of human cDNA whose protein product responsible for synthesis GM3. The cloned spanned 2,359 base pairs, with an open reading frame encoding 362 amino acids predicted molecular mass 41.7 kDa. deduced primary structure shows features characteristic sialyltransferase family, including type II transmembrane topology sialylmotifs L at center S C-terminal region. An...
Clostridium botulinum neurotoxins (BoNTs) act on nerve endings to block acetylcholine release. Their potency is due their enzymatic activity and selective high affinity binding neurons. Although there are many pieces of data available the receptor for BoNT, little attempt has been made characterize receptors BoNT/C BoNT/D. For this purpose, we prepared recombinant carboxyl-terminal domain heavy chain (H)C then examined its capability rat brain synaptosomes treated with enzymes heating....
Current guidelines recommended adequate nutritional support for patients with liver cirrhosis to improve clinical outcome and quality of life (QOL). However, these evidences were obtained more than 10 years ago when malnutrition prevailed. In recent years, the impact obesity on damage carcinogenesis has grown. We attempted elucidate state QOL in present cirrhotics.A research group supported by Ministry Health, Labor Welfare Japan recruited 294 cirrhotics between 2007 2011. Subjects comprised...
CC chemokine receptor 3 (CCR3) belongs to the G protein-coupled family and is highly expressed in eosinophils basophils. CCR3 essential for recruiting into lung. Moreover, was found serum of colorectal cancer patients higher than control group. Therefore, will be a useful target asthma diagnosis. This study developed specific sensitive monoclonal antibody (mAb) mouse (mCCR3), which flow cytometry using Cell-Based Immunization Screening method. The established anti-mCCR3 mAb, C3Mab-3 (rat...
Neolacto-series gangliosides having linear poly-N-acetyl-lactosaminyl oligosaccharide structure have been demonstrated to be increased characteristically during granulocytic differentiation of human promyelocytic leukemia cell line HL-60 cells induced by dimethyl sulfoxide or retinoic acid (Nojiri, H., Takaku, F., Tetsuka, T., Motoyoshi, K., Miura, Y., and Saito, M. (1984) Blood 64, 534-541). When were cultured in the presence neolacto-series prepared from mature granulocytes, found...
The phosphatase inhibitor okadaic acid was found to induce cell cycle arrest of human myeloid leukemic lines HL-60 and U937 in a concentration- time-dependent manner. Exposure low concentrations (2-8nM) for 24-48 hr caused greater than 70% cells at G2/M, with up 40% the arrested early mitosis. Cell viability decreased rapidly after 48 treatment, morphological DNA structure analysis indicated that this primarily due induction apoptosis. mitosis by 8 nM could be highly enriched density...
A new bacterial sialidase (N-acetylneuraminate glycohydrolase, EC 3.2.1.18) isolated from the culture filtrate of Arthrobacter ureafaciens was characterized in detail with respect to its action on sialoglycolipids. Strong electrolytes had a reversible inhibitory effect enzyme brain gangliosides accordance Debye-Huckel ionic environment activity, and resulted an acidic shift broadening pH optimum. Both non-ionic detergents markedly enhanced enzymic activity gangliosides, caused optimum this...
The epidermal growth factor receptor (EGFR) is a type I transmembrane protein, which member of the human (HER) family tyrosine kinases. EGFR crucial mediator cell and differentiation forms homodimers or heterodimers with other HER members to activate downstream signaling cascades. We previously established an anti-human (hEGFR) monoclonal antibody (mAb), clone EMab-134 (mouse IgG1), by immunizing mice ectodomain hEGFR. In this study, subclass was converted from IgG1 IgG2a (134-mG2a) further...
The epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein. Although EGFR physiologically essential in normal cells, it contributes to tumor malignancy through gene amplification and/or protein overexpression, which augment signaling cascades cells. We previously developed an anti-human (hEGFR) monoclonal antibody (mAb), EMab-134 (mouse IgG1, kappa), detects hEGFR and dog (dEGFR) with high sensitivity specificity. mouse IgG2a version of (134-mG2a) has antitumor effects...