A5072221176- Retinal Diseases and Treatments
- Retinal Development and Disorders
- Cellular transport and secretion
- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- Erythrocyte Function and Pathophysiology
- Glaucoma and retinal disorders
- Pancreatic function and diabetes
- Retinal Imaging and Analysis
- Bee Products Chemical Analysis
- Protist diversity and phylogeny
- Connexins and lens biology
- Neuroinflammation and Neurodegeneration Mechanisms
- Peptidase Inhibition and Analysis
- Renal and related cancers
- Saffron Plant Research Studies
- RNA Interference and Gene Delivery
- Retinal and Optic Conditions
- Antioxidant Activity and Oxidative Stress
- Endoplasmic Reticulum Stress and Disease
- Hedgehog Signaling Pathway Studies
- Blood Coagulation and Thrombosis Mechanisms
- Plant Gene Expression Analysis
- Heme Oxygenase-1 and Carbon Monoxide
- Ferroptosis and cancer prognosis
Gifu Pharmaceutical University
2019-2024
Cornell University
2016-2024
Weill Cornell Medicine
2018-2024
Hokkaido University
2011-2020
Ophthalmology Associates (United States)
2019
Abstract Age-related macular degeneration (AMD) is the leading cause of blindness among elderly. Dry AMD has unclear etiology and no treatment. Lipid-rich drusen are hallmark dry AMD. An mouse model insights into drusenogenesis keys to better understanding this disease. Chloride intracellular channel 4 (CLIC4) a pleomorphic protein regulating diverse biological functions. Here we show that retinal pigment epithelium (RPE)-specific Clic4 knockout mice exhibit full spectrum functional...
Abstract Chloride intracellular channel 4 (CLIC4) is a mammalian homologue of EXC-4 whose mutation associated with cystic excretory canals in nematodes. Here we show that CLIC4-null mouse embryos exhibit impaired renal tubulogenesis. In both developing and developed kidneys, CLIC4 specifically enriched the proximal tubule epithelial cells, which important for luminal delivery, microvillus morphogenesis, endolysosomal biogenesis. Adult tubules display aberrant dilation. MDCK 3D cultures,...
Non-exudative age-related macular degeneration (AMD) is an irreversibly progressive retinal degenerative disease characterized by dysfunction and loss of pigment epithelium (RPE). It has been suggested that impaired phagocytosis the RPE involved in progression non-exudative AMD, but mechanism not fully clear. In this study, we investigated effect lipid droplet accumulation on function. Compared to young mice, expression droplet-associated proteins increased RPE-choroidal complex, was...
Abstract Background Blue light exposure is known to induce reactive oxygen species (ROS) production and increased endoplasmic reticulum stress, leading apoptosis of photoreceptors. Maqui berry ( Aristotelia chilensis ) a fruit enriched in anthocyanins, for beneficial biological activities such as antioxidation. In this study, we investigated the effects extract (MBE) its constituents on subcellular damage induced by blue irradiation mouse retina-derived 661W cells. Methods We evaluated MBE...
Abstract The cornea is directly exposed to cigarette smoke, and smoking a risk factor for several corneal diseases including dry eye syndrome. Currently, heated tobacco products (HTPs) are widely used as substitutes around the world. In present study, we investigated molecular mechanism(s) leading cellular injury induced by smoke extract (CSE) or HTPs. Exposure CSE perturbed formation of tight junctions, an increase in cell volume, decrease transepithelial electrical resistance (TER) human...
Abstract Vacuolar protein sorting 35 (VPS35), the core component of retromer complex which regulates endosomal trafficking, is genetically linked with Parkinson’s disease (PD). Impaired vision a common non-motor manifestation PD. Here, we show mouse retinas VPS35-deficient rods exhibit synapse loss and visual deficit, followed by progressive degeneration concomitant emergence Lewy body-like inclusions phospho-α-synuclein (P-αSyn) aggregation. Ultrastructural analyses reveal accumulate...
Peripherin 2 (PRPH2) is a tetraspanin protein concentrated in the light-sensing cilium (called outer segment) of vertebrate photoreceptor. The mechanism underlying ciliary targeting PRPH2 and etiology cone dystrophy caused by mutations remain elusive. Here we show that late endosome (LE) main waystation critically sorts newly synthesized to cilium. expressed luminal membrane LE. We delineate multiple C-terminal motifs distinctively regulate its LE through ubiquitination binding ESCRT...
Abstract Dysregulation in the extracellular matrix (ECM) microenvironment surrounding retinal pigment epithelium (RPE) has been implicated etiology of proliferative vitreoretinopathy and age-related macular degeneration. The regulation ECM remodeling by RPE cells is not well understood. We show that membrane-type metalloproteinase 14 (MMP14) central to degradation at focal adhesions human ARPE19 cells. degradative activity, but assembly, adhesion regulated chloride intracellular channel 4...
TSPO2 (translocator protein 2) is a transmembrane specifically expressed in late erythroblasts and has been postulated to mediate intracellular redistribution of cholesterol. We identified as the causative gene for HK (high-K+) trait with immature red cell phenotypes dogs investigated effects defects on erythropoiesis mutation Tspo2 knockout (Tspo2−/−) mouse models. Bone marrow–derived from showed increased binucleated apoptotic cells at various stages maturation shed large nuclei incomplete...
Age-related macular degeneration (AMD) is the principal cause of permanent blindness among elderly individuals worldwide. Chronic inflammation in subretinal space associated with a progression exudative AMD. Progranulin (PGRN) growth factor secreted from myeloid cells and plays an important role controlling lysosomal function. A deficiency PGRN leads to neurons central nervous system. The purpose this study was investigate played by size choroidal neovascularization (CNV) laser-induced CNV...
The corneal epithelium is located at the outermost layer of ocular surface and continuously exposed to environmental factors, such as ultraviolet (UV) radiation from sunlight. UV irradiation causes excessive production reactive oxygen species (ROS) in cells, which results oxidative damage membrane-bound organelles mitochondria, eventually leading cell death. Crocetin, a natural carotenoid found plants, has various biological properties including antioxidant activity. In this study, we...
To explore the roles of conserved YXXΦ-type motif in erythroid-specific N-terminal stretch anion exchanger 1 (AE1), cell surface expression and internalization various mutants derived from murine erythroid AE1 tagged with an enhanced green fluorescent protein extracellular FLAG (EGFP-mAE1Flag) were explored K562 HEK293 cells. EGFP-mAE1Flag showed rapid internalization, association internalizations transferrin endogenous chaperone-like glycophorin A Disruption Y72VEL sequence markedly reduced...
Objective: This study was conducted to evaluate the effects of anti-vascular endothelial growth factor (VEGF) antibody (bevacizumab) on vascular leakage and fibrosis in a monkey choroidal neovascularization (CNV) model. The relationship between fibrotic tissue subretinal hyper-reflective material (SHRM), optical coherence tomography (OCT) images, also investigated. Methods: Experimental CNV induced male cynomolgus monkeys by laser photocoagulation. Intravitreal injection bevacizumab at 0.5...
The corneal epithelium is continuously exposed to oxygen, light, and environmental substances. Excessive exposure those stresses thought be a risk factor for eye diseases. Photokeratitis damage the resulting in painful condition caused by unprotected UV rays, usually from sunlight, often found people who spend long time outdoors. In modern life, human eyes are artificial light light-emitting diode (LED) displays of computers smartphones, it has been shown that short-wavelength (blue) LED can...
Neovascular glaucoma (NVG) is caused by the formation of new blood vessels in angle, iris, and cornea retinal ischemic disease, such as proliferative diabetic retinopathy (PDR) vein occlusion (RVO), which can reduce visual acuity. However, pathophysiological symptoms NVG are still not well understood because there no model for cornea. The aim this study was to investigate involvement during a murine ischemia. We evaluated changes intraocular pressure (IOP) pathological anterior eye segment...
Background: Sarcopenia is a progressive skeletal muscle disorder characterized by the loss of mass and function, resulting in physical disability mortality. Although sarcopenia impacts large proportion elderly individuals, no effective treatment for this disease has yet been identified. The excessive production reactive oxygen species (ROS) can damage tissues promote aging, daily use dietary antioxidants be maintaining health. Propolis, natural substance that collected honey bees, used as...