A5068610741- Erythrocyte Function and Pathophysiology
- Pancreatic function and diabetes
- Blood properties and coagulation
- Metabolism and Genetic Disorders
- Neonatal Health and Biochemistry
- Ion Transport and Channel Regulation
- Amino Acid Enzymes and Metabolism
- Hemoglobinopathies and Related Disorders
- Glycosylation and Glycoproteins Research
- Neuroscience and Neuropharmacology Research
- Blood groups and transfusion
- Ion channel regulation and function
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Animal Genetics and Reproduction
- Barrier Structure and Function Studies
- Cellular transport and secretion
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- Lipid Membrane Structure and Behavior
- Genetic and phenotypic traits in livestock
- Enzyme function and inhibition
- Vector-borne infectious diseases
- Psoriasis: Treatment and Pathogenesis
- RNA regulation and disease
Hokkaido University
2009-2024
Waseda University
2005-2006
The University of Tokyo
1996-2002
Tokyo University of Agriculture
1997
Asahikawa Medical College Hospital
1995
Rush University
1994
Osaka University
1990
We studied bovine subjects that exhibited a moderate uncompensated anemia with hereditary spherocytosis inherited in an autosomal incompletely dominant mode and retarded growth. Based on the results of SDS-PAGE, immunoblotting, electron microscopic analysis by freeze fracture method, we show here proband red cells lacked band 3 protein completely. Sequence cDNA genomic DNA showed C --> T substitution resulting nonsense mutation (CGA TGA; Arg Stop) at position corresponding to codon 646 human...
Data from an exDeriment for each type of cells.The control values K,,, and Vmax are 9.1 350.9 in high K+ cells, 10.5 a i d 118.1 low K' ckils, respectively.
The Na,K-ATPase of red cells from high K+ and low dogs was studied immunologically by using antibodies raised against dog kidney enzyme.Anti-a subunit IgGs, which also recognized a(+) brain enzyme, identified the larger erthrocyte as a homogeneous polypeptide with M , = 96,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed immunoblotting.In addition, erythrocyte Na,K-ATPase, purified immunoaffinity chromatography monoclonal antibody-coupled column, showed identity its...
The present study demonstrated that dog reticulocytes had considerable amounts of (Na,K)-ATPase, but lost it rapidly during maturation into erythrocytes.Furthermore, from dogs possessing erythrocytes characterized with high (Na,K)-ATPase activity and K, low Na concentrations (HK dogs; Maede, Y., Inaba, M., Taniguchi, N. (1983) Blood 61,493-499) more ouabain binding sites than cells normal (LK dogs).Our results were as follows: i) maximal capacities (B-) for at equilibrium approximately 0...
We have recently characterized IQI/Jic mice as a model for Sjogren's syndrome (SS), chronic autoimmune disease in humans. In SS, local lymphocytic infiltrations into salivary and lacrimal glands frequently develop to the involvement of systemic exocrine nonexocrine organs, mechanism progression this remains obscure. Herein, we report identification an autoantigen shared by various target organs mice. Polypeptides identified based on immunorecognition autoantibodies sera from affected with...
Canine degenerative myelopathy (DM) is a progressive neurodegenerative disease that frequently found in Pembroke Welsh Corgi (PWC) dogs. DM potentially spontaneous animal model for human amyotrophic lateral sclerosis (ALS) because of similar lesions and the involvement superoxide dismutase 1 (SOD1) mutation. However, ventral horn lesion has not been characterized detail. Glutamate excitotoxicity due to deficiency glutamine-glutamate cycle implicated neuron death ALS. Thus, we examined 5 PWC...
Polypoid cystitis is a rare disease of the urinary bladder in dogs characterized by chronic inflammation, epithelial proliferation, and development polypoid mass or masses without histopathologic evidence neoplasia. The ultrasonographic appearances eight with are described. Ultrasonography confirmed presence all patients. Ultrasonographic findings mucosal projections to pedunculated variable size shape. Although tends be located cranioventral mucosa, polyps also could arise craniodorsal...
Claudin-16 is one of the tight junction protein claudins and has been shown to contribute reabsorption divalent cations in human kidney. In cattle, total deficiency claudin-16 causes severe renal tubular dysplasia without aberrant metabolic changes cations, suggesting that bovine some roles tubule formation paracellular transport are somewhat different from those expected pathology disease. As first step clarify these roles, we examined expression distribution several other major claudin...
Various mutations in the AE1 (anion exchanger 1, band 3) gene cause dominant hereditary spherocytosis, a common congenital hemolytic anemia associated with deficiencies of different degrees and loss mutant protein from red blood cell membranes. To determine mechanisms underlying decreases levels, we employed K562 HEK293 lines Xenopus oocytes together bovine wild-type an R664X nonsense responsible for spherocytosis to analyze expression, turnover, intracellular localization. R664X-mutant...
TSPO2 (translocator protein 2) is a transmembrane specifically expressed in late erythroblasts and has been postulated to mediate intracellular redistribution of cholesterol. We identified as the causative gene for HK (high-K+) trait with immature red cell phenotypes dogs investigated effects defects on erythropoiesis mutation Tspo2 knockout (Tspo2−/−) mouse models. Bone marrow–derived from showed increased binucleated apoptotic cells at various stages maturation shed large nuclei incomplete...
SUMMARY Cats given dl -methionine (1 g/kg of body weight/day) developed severe hemolytic anemia with marked increase methemoglobin (MetHb) concentration and Heinz-body formation at treatment-day 6 to 10. fed 0.5 g me-thionine/kg for 52 days had a moderate methemoglobinemia treatment 17 31, but thereafter recovered from the despite continuation methionine feeding, indicating an adaptation cats. In vitro, significant ( P < 0.01) increases MetHb were observed when rbc incubated plasma cats...
Psoriasis is a chronic inflammatory skin disease characterized by epidermal hyperplasia and cellular infiltration. Studies have shown that development depends on proinflammatory cytokines, such as interleukin (IL)-23 IL-17. It has been suggested IL-23 produced innate immune cells, macrophages, stimulates subset of helper T cells to release IL-17, promoting neutrophil recruitment keratinocyte proliferation. However, recent studies revealed the crucial role γδT in psoriasis pathogenesis...
Background Interleukin (IL)-17-producing γδT (γδT17) cells mediate inflammatory responses in barrier tissues. Dysregulated γδT17 cell activation can lead to the overproduction of IL-17 and IL-22 development diseases, including psoriasis. IL-23 IL-1β are known synergistically activate cells, but regulatory mechanisms have not been fully elucidated. This study aimed reveal contribution cytokine tumor necrosis factor-like ligand 1A (TL1A) psoriasis development. Methods Anti-TL1A antibody was...
Erythrocyte protein 4.1 is a cytoplasmic that possesses protein-saccharide modification structure, an O-N-acetyl-D-glucosamine (GlcNAc) moiety. We determined the amino acid sequence of proteolytic fragment containing O-GlcNAc moiety after labeling saccharide with [3H]galactose in presence bovine milk galactosyltransferase. Glycosylation appears to occur on one or more serine threonine residues following sequence:...
Erythrocyte membranes from goat contain a considerable amount, more than 10% of the total glycoprotein with Mr = 155,000 (gp155) on sodium dodecyl sulfate-polyacrylamide electrophoresis gel. This report describes first isolation and characterization gp155. gp155 has major trypsin-sensitive sites at each side plasma membrane to generate membrane-bound fragments, indicating that spans lipid bilayer several times. protein consists single polypeptide containing about 1,200 amino acid residues...