A5101591164- Erythrocyte Function and Pathophysiology
- Pancreatic function and diabetes
- Neuroscience and Neuropharmacology Research
- Adipose Tissue and Metabolism
- Retinal Development and Disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Cellular transport and secretion
- Amino Acid Enzymes and Metabolism
- Neonatal Health and Biochemistry
- Immunotherapy and Immune Responses
- Ion channel regulation and function
- Glycosylation and Glycoproteins Research
- Retinoids in leukemia and cellular processes
- T-cell and B-cell Immunology
- Trace Elements in Health
- Biopolymer Synthesis and Applications
- Neurological diseases and metabolism
- Virus-based gene therapy research
- Blood properties and coagulation
- Photoreceptor and optogenetics research
- Zoonotic diseases and public health
- Acute Ischemic Stroke Management
- Retinal Diseases and Treatments
- Pharmaceutical and Antibiotic Environmental Impacts
- Metallurgy and Material Forming
Hirosaki University
1990-2024
Waseda University
2022-2024
Hokkaido University
2008-2023
Fuji Heavy Industries Health Insurance Society Ota Memorial Hospital
2021-2023
Chubu University
2023
Japanese Red Cross Medical Center
2023
Saitama University
2017-2022
Kyushu University
2016-2021
Niigata University
2021
National Hospital Organization
2019
We studied bovine subjects that exhibited a moderate uncompensated anemia with hereditary spherocytosis inherited in an autosomal incompletely dominant mode and retarded growth. Based on the results of SDS-PAGE, immunoblotting, electron microscopic analysis by freeze fracture method, we show here proband red cells lacked band 3 protein completely. Sequence cDNA genomic DNA showed C --> T substitution resulting nonsense mutation (CGA TGA; Arg Stop) at position corresponding to codon 646 human...
Interphotoreceptor retinoid-binding protein (IRBP) secreted by photoreceptors plays a pivotal role in photoreceptor survival with an unknown mechanism. A mutation the human IRBP has been linked to retinitis pigmentosa, progressive retinal degenerative disease. Mice lacking display severe early and degeneration. However, signaling pathway(s) leading death IRBP-deficient mice remains poorly understood. Here, we show that amounts of tumor necrosis factor-α (TNF-α) interphotoreceptor matrix...
Adiponectin, a circulating adipocyte-derived hormone, exerts beneficial actions on hearts subjected to ischemia-reperfusion injury. Adiponectin exists in plasma as three different oligomeric forms: trimer, hexamer and high molecular weight. This study investigated the expression myocardial accumulation of adiponectin murine model injury.Wild-type deficient mice were left anterior descending artery ligation followed by reperfusion. Plasma levels analyzed ELISA heart was determined...
Although recent studies suggest involvement of glutathione transferase (GST) blood vessels in vasodilation by nitroglycerin, GST forms remain to be studied. In this study, three (pI values 8.3, 6.6, and 4.8) were purified from human aorta four 6.0, 5.6, 5.3, 4.6) the heart affinity chromatography followed chromatofocusing. The major form both was identified as GST-pi, other five immunologically related GST-mu, suggesting that belong Mu class. Among nine forms, including Alpha class liver, pI...
Objective: With the increasing prevalence of mechanical thrombectomy (MT) for large vessel occlusion strokes, encountering unruptured cerebral aneurysms (uANs) in MT target vessels has become more common, necessitating case accumulation to establish safety guidelines such cases. In this study, we aimed review and present cases uAN associated with at our hospital. Methods: Among 320 patients who underwent strokes hospital between January 2018 December 2021, selected analyzed various...
Abstract Objective —To examine stress-related neurohormonal and metabolic effects of butorphanol, fentanyl, ketamine administration alone in combination with medetomidine dogs. Animals —10 Beagles. Procedure —5 dogs received either butorphanol (0.1 mg/kg), fentanyl (0.01 or (10 mg/kg) IM a crossover design. Another 5 (0.02 saline (0.9% NaCl) solution mL/kg) similar Blood samples were obtained for 6 hours following the treatments. Norepinephrine, epinephrine, cortisol, glucose, insulin,...
Abstract: A number of C‐type lectins on antigen‐presenting cells play an important role in regulating innate immunity. Previously, we identified the mouse (dectin‐1, and dectin‐2) human DECTIN‐1. To identify DECTIN‐2, employed degenerative polymerase chain reaction‐based cDNA cloning using RNA from Langerhans cell (LC)‐like dendritic (DCs). This process yielded a encoding lectin with 66.5% amino acid sequence homology to dectin‐2, same gene reported by Kanazawa et al. (J Invest Dermatol...
The retinal pigment epithelium (RPE)-dependent visual cycle provides 11-cis-retinal to opsins in the photoreceptor outer segments generate functional pigments that initiate phototransduction response light stimuli. Both RPE65 isomerase of and rhodopsin have recently been identified as critical players mediating light-induced degeneration. These findings suggest expression function need be coordinately controlled sustain normal vision protect retina from photodamage. However, mechanism...
Plasma lipoprotein cholesterol in 64 clinically healthy Shetland sheepdogs was evaluated to assess whether the breed is more susceptible hypercholesterolemia. The incidence of hypercholesterolemia clearly higher and mean plasma level significantly than control dogs. Blood biochemical examinations did not evidence abnormalities, which imply causative disorders, thyroid hormone levels were different from controls. These results suggest that cholesterolemia a primary disorder. Cholesterol...
Abstract In glaucoma, although axonal injury drives retinal ganglion cell (RGC) death, little is known about the underlying pathomechanisms. To provide new mechanistic insights and identify biomarkers, we combined latest non-targeting metabolomics analyses to profile altered metabolites in mouse whole retina 2, 4, 7 days after optic nerve crush (NC). Ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry Fourier transform covering wide spectrum of...
A metal–organic framework (MOF) consisting of Cu-benzenetricarboxylic acid was processed under ultrahigh pressure (5 GPa) and at temperature up to 500 °C. The products were characterized with TEM, FTIR, XAFS. decomposition the MOF started 200 °C 5 GPa. This much lower than that in vacuum. Single-nanometer Cu nanoparticles obtained carbon matrix, which significantly smaller particles prepared ambient pressure. catalytic activity for Huisgen cycloaddition examined, sample GPa showed a improved...
A 14-year-old mixed-breed dog was examined because of severe absolute erythrocytosis (PCV, 70%). Plasma erythropoietin (EPO) concentration consistently high, even though results arterial blood gas analyses were normal. Radiography, ultrasonography, urinalysis, and serum biochemical did not reveal any cardiac, pulmonary, or renal abnormalities that could cause the erythrocytosis, secondary to inappropriate EPO secretion diagnosed. The PCV maintained at approximately 60% by means periodic...
The Rcs phosphorelay signal transduction system of Escherichia coli controls genes for capsule production and many other envelope-related functions is implicated in biofilm formation. outer-membrane lipoprotein RcsF an essential component the system. Mislocalization to periplasm or cytoplasmic membrane leads high activation system, suggesting that by interacting with component(s) activating This consistent result reported Cho et al. (Cell 159, 1652–1664, 2014) showing interacts periplasmic...
TSPO2 (translocator protein 2) is a transmembrane specifically expressed in late erythroblasts and has been postulated to mediate intracellular redistribution of cholesterol. We identified as the causative gene for HK (high-K+) trait with immature red cell phenotypes dogs investigated effects defects on erythropoiesis mutation Tspo2 knockout (Tspo2−/−) mouse models. Bone marrow–derived from showed increased binucleated apoptotic cells at various stages maturation shed large nuclei incomplete...
The effects of several factors related to lipids on bovine adipocyte differentiation were investigated in primary culture.Adipocyte was assessed by development glycerol−3−phosphate dehydrogenase(GPDH)activity and morphological observation.Addition triglyceride mixture(Intralipid), caprylic acid very low−, low− high−density lipoproteins(VLDL, LDL HDL)stimulated preadipocyte serum−free condition.Especially, VLDL strongly increased both cell protein contents GPDH activity, suggesting that it...