A5002310524- Sulfur Compounds in Biology
- Nitric Oxide and Endothelin Effects
- Genomics, phytochemicals, and oxidative stress
- Neuroinflammation and Neurodegeneration Mechanisms
- Free Radicals and Antioxidants
- Neuroscience and Neuropharmacology Research
- Redox biology and oxidative stress
- Adenosine and Purinergic Signaling
- Enzyme Production and Characterization
- Biochemical effects in animals
- Botulinum Toxin and Related Neurological Disorders
- Biofuel production and bioconversion
- Air Quality and Health Impacts
- Polysaccharides and Plant Cell Walls
- Cellular transport and secretion
- Hereditary Neurological Disorders
- Mitochondrial Function and Pathology
- Neuroscience of respiration and sleep
- Receptor Mechanisms and Signaling
- Renin-Angiotensin System Studies
- Insect and Pesticide Research
- Neurological disorders and treatments
- Microbial Metabolites in Food Biotechnology
- Protein Hydrolysis and Bioactive Peptides
- Nicotinic Acetylcholine Receptors Study
Osaka Metropolitan University
2022-2024
Osaka Prefecture University
2014-2023
Tokyo Metropolitan University
2022-2023
Osaka University
2010
National Heart Lung and Blood Institute
1999
National Institutes of Health
1999
Tottori University
1996
Significance Reactive sulfur-containing compounds, such as l -cysteine hydropersulfide (CysSSH), reportedly form in mammals. However, the biological relevance of these reactive sulfur species remains unclear. We determined that CysSSH was synthesized from cystine by cystathionine β-synthase and γ-lyase, which turn may contribute to high levels glutathione (>100 μM) other derivatives peptides/proteins formed cells, tissues, plasma mice humans. Compared with hydrogen sulfide, were superior...
Abstract Cysteine hydropersulfide (CysSSH) occurs in abundant quantities various organisms, yet little is known about its biosynthesis and physiological functions. Extensive persulfide formation apparent cysteine-containing proteins Escherichia coli mammalian cells believed to result from post-translational processes involving hydrogen sulfide-related chemistry. Here we demonstrate effective CysSSH synthesis the substrate l -cysteine, a reaction catalyzed by prokaryotic cysteinyl-tRNA...
A nitrated guanine nucleotide, 8-nitroguanosine 3′,5′-cyclic monophosphate (8-nitro-cGMP), is formed via nitric oxide (NO) and causes protein <i>S</i>-guanylation. However, intracellular 8-nitro-cGMP levels mechanisms of formation <i>S</i>-guanylation are yet to be identified. In this study, we precisely quantified NO-dependent in C6 glioma cells liquid chromatography-tandem mass spectrometry. Treatment with <i>S</i>-nitroso-<i>N</i>-acetylpenicillamine led a rapid, transient increase cGMP,...
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)(2) is a classic glycolytic enzyme that also mediates cell death by its nuclear translocation under oxidative stress. Meanwhile, we previously presented stress induced disulfide-bonded GAPDH aggregation in vitro. Here, propose aggregate formation might participate stress-induced both vitro and vivo. We show human amyloid-like depends on the active site cysteine-152 (Cys-152) In SH-SY5Y neuroblastoma, treatment with dopamine decreases viability...
Abstract The mammalian brain is highly vulnerable to oxygen deprivation, yet the mechanism underlying brain’s sensitivity hypoxia incompletely understood. Hypoxia induces accumulation of hydrogen sulfide, a gas that inhibits mitochondrial respiration. Here, we show that, in mice, rats, and naturally hypoxia-tolerant ground squirrels, inversely related levels sulfide:quinone oxidoreductase (SQOR) capacity catabolize sulfide. Silencing SQOR increased hypoxia, whereas neuron-specific expression...
Clostridium botulinum neurotoxins (BoNTs) act on nerve endings to block acetylcholine release. Their potency is due their enzymatic activity and selective high affinity binding neurons. Although there are many pieces of data available the receptor for BoNT, little attempt has been made characterize receptors BoNT/C BoNT/D. For this purpose, we prepared recombinant carboxyl-terminal domain heavy chain (H)C then examined its capability rat brain synaptosomes treated with enzymes heating....
Plants are exposed to hydrogen sulfide (H2S) both exogenously, as it exists a pollutant gas in the environment, and endogenously, is synthesized cells. H2S has recently been found function gaseous signaling molecule, but its cascade remains unknown. Here, we examined H2S-mediated guard cell Arabidopsis. The donor GYY4137 (morpholin-4-ium-4-methoxyphenyl [morpholino] phosphinodithioate) induced stomatal closure, which peaked after 150 min at 1 µM or 90 10 100 µM. After reaching maximal...
Polysaccharides were extracted from Caulerpa lentillifera by treating with water and then purified size-exclusion chromatography. The polysaccharides, termed SP1, found to be sulfated xylogalactans a molecular mass of more than 100 kDa. Adding SP1 murine macrophage RAW 264.7 cells increased the production nitric oxide (NO) in dose-dependent manner. NO was immunoblotting RT-PCR analyses synthesized an inducible synthase. caused degradation IκB-α nuclear translocation factor (NF)-κB subunit...
The physiological importance of reactive sulfur species (RSS) such as cysteine hydropersulfide (CysSSH) has been increasingly recognized in recent years. We have established a metabolomics analysis by using RSS metabolic profiling, which revealed appreciable amounts generated endogenously and ubiquitously both prokaryotic eukaryotic organisms. chemical nature these polysulfides is not fully understood, however, because their or complicated redox-active properties. In our study here, we...
Although hydrogen sulfide (H2S) is an endogenous signaling molecule with antioxidant properties, it also cytotoxic by potently inhibiting cytochrome c oxidase and mitochondrial respiration. Paradoxically, the primary route of H2S detoxification thought to occur inside matrix via a series relatively slow enzymatic reactions that are unlikely compete its rapid inhibition oxidase. Therefore, alternative or complementary cellular mechanisms predicted exist. Here, superoxide dismutase [Cu-Zn]...
8-Nitro-cGMP (8-nitroguanosine 3′,5′-cyclic monophosphate) is a nitrated derivative of cGMP, which can function as unique electrophilic second messenger involved in regulation an antioxidant adaptive response cells. In the present study, we investigated chemical and biochemical regulatory mechanisms 8-nitro-cGMP formation, with particular focus on roles ROS (reactive oxygen species). Chemical analyses demonstrated that peroxynitrite-dependent oxidation myeloperoxidase-dependent nitrite...
Tools to enable spatiotemporally controlled upregulation of supersulfides, which are highly reactive, unstable sulfur species, needed study the pathophysiological roles post-translational protein modification with catenated atoms. Here, we set out design N,N-diethylaminocoumarin (DEAC)-based visible-light-responsive N-acetylcysteine persulfide donors (NAC-SS-DEAC), and serendipitously found that upon visible light irradiation, they donate a sulfane (S0) atom nucleophiles, including thiols...
β-1,3-Xylan was prepared from the green alga, Caulerpa lentillifera, and hydrolyzed to oligosaccharides by a mild acid treatment. The average degree of polymerization about 5. reduced number viable human breast cancer MCF-7 cells in dose-dependent manner, induced chromatin condensation degradation poly ADP-ribose polymerase, indicating that they apoptosis cells.