A5005225033- Zebrafish Biomedical Research Applications
- Neuroscience and Neuropharmacology Research
- Developmental Biology and Gene Regulation
- Lipid Membrane Structure and Behavior
- Neural dynamics and brain function
- Genetic and Kidney Cyst Diseases
- Wnt/β-catenin signaling in development and cancer
- Neuroscience of respiration and sleep
- Connective tissue disorders research
- Neurogenesis and neuroplasticity mechanisms
- Olfactory and Sensory Function Studies
- Retinal Development and Disorders
- Sleep and Wakefulness Research
- Neonatal and fetal brain pathology
- Memory and Neural Mechanisms
- EEG and Brain-Computer Interfaces
- Cell Adhesion Molecules Research
- Proteoglycans and glycosaminoglycans research
- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Cell Image Analysis Techniques
- Cerebrospinal fluid and hydrocephalus
- Advanced Fluorescence Microscopy Techniques
- Fetal and Pediatric Neurological Disorders
- Congenital heart defects research
Inserm
2013-2018
Harvard University
2018
Centre National de la Recherche Scientifique
2013-2018
Assistance Publique – Hôpitaux de Paris
2018
Sorbonne Université
2013-2018
Institut du Cerveau
2013-2018
MRC Toxicology Unit
1986
Abstract Defects in cerebrospinal fluid (CSF) flow may contribute to idiopathic scoliosis. However, the mechanisms underlying detection of CSF central canal spinal cord are unknown. Here we demonstrate that flows bidirectionally along antero-posterior axis zebrafish embryos. In cfap298 tm304 mutant, reduction cilia motility slows transport posteriorly down and abolishes spontaneous activity CSF-contacting neurons (CSF-cNs). Loss sensory Pkd2l1 channel nearly CSF-cN calcium single opening....
The zebrafish larva stands out as an emergent model organism for translational studies involving gene or drug screening thanks to its size, genetics, and permeability. At the larval stage, locomotion occurs in short episodes punctuated by periods of rest. Although phenotyping behavior is a key component large-scale screens, it has not yet been automated this system. We developed ZebraZoom, program automatically track larvae identify maneuvers many animals performing discrete movements. Our...
Organ development depends on the integration of coordinated long-range communication between cells. The cerebrospinal fluid composition and flow properties regulate several aspects central nervous system development, including progenitor proliferation, neurogenesis, migration [1-3]. One understudied component fluid, described over a century ago in vertebrates, is Reissner fiber. This extracellular thread forming early results from assembly SCO-spondin protein third fourth brain ventricles...
Abstract Chemical and mechanical cues from the cerebrospinal fluid (CSF) can affect development function of central nervous system (CNS). How such are detected relayed to CNS remains elusive. Cerebrospinal fluid-contacting neurons (CSF-cNs) situated at interface between CSF ideally located convey information local networks. In spinal cord, these GABAergic expressing PKD2L1 channel extend an apical extension into ascending axon in cord. zebrafish mouse CSF-cNs originate two distinct...
Imaging intracellular calcium concentration via reporters that change their fluorescence properties upon binding of calcium, referred to as imaging, has revolutionized our way probe neuronal activity non-invasively. To reach neurons densely located deep in the tissue, optical sectioning at high rate acquisition is necessary but difficult achieve a cost effective manner. Here we implement an accessible solution relying on HiLo microscopy provide robust with frame vivo. We show large signals...
The recent development of genetically encoded calcium indicators enables monitoring in vivo the activity neuronal populations. Most analysis these transients relies on linear regression based sensory stimulus applied or behavior observed. To estimate basic properties functional neural circuitry, we propose a network approach to imaging recorded at single cell resolution. Differently from previous cross-correlation, used Granger-causality estimates infer information propagation between...
ABSTRACT The distribution of the extracellular matrix molecules fibronectin, laminin and entactin was studied in frozen sections -day mouse embryos order to relate their presence neural crest cell migration. It found that all three components were present basement mem-branes, being mainly restricted these. Fibronectin at high levels membranes spaces throughout embryo, including regions is known affect migration a variety systems, so its the.embryo time may indicate it influencing movement....
ABSTRACT The earliest stage of neural crest cell (NCC) migration is characterized by an epitheliomesenchymal transformation, as the cells leave tube. There evidence that in a number systems this transformation accompanied alteration or depletion associated basement membranes. This study examines ultrastructural relationship between mouse NCCs and adjacent membranes during stages from Basement were identified transmission electron microscopy (TEM) immunofluorescence using antibodies to...
Abstract Flow of cerebrospinal fluid (CSF) may contribute to spine morphogenesis, as mutations affecting both cilia motility and CSF flow lead scoliosis 1 . However, the mechanisms underlying detection in central canal spinal cord remain elusive. Here we used full-field optical coherence tomography (FF-OCT) bead tracking demonstrate that flows bidirectionally along antero-posterior axis zebrafish embryos. In mutant cfap298 tm304 , previously known kurly reduction slows transport down length...
We report the first imaging and quantification of cerebrospinal fluid (CSF) dynamics within spinal cord zebrafish larvae. It provides a basis for understanding how CSF selectively transports instructive signals during development morphogenesis.