A5024986551- Zebrafish Biomedical Research Applications
- Neural dynamics and brain function
- Turtle Biology and Conservation
- Marine Ecology and Invasive Species
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Neurobiology and Insect Physiology Research
- Spider Taxonomy and Behavior Studies
- Cellular Mechanics and Interactions
- Micro and Nano Robotics
- Subterranean biodiversity and taxonomy
- Nonlinear Dynamics and Pattern Formation
- Neuroscience and Neuropharmacology Research
- Modular Robots and Swarm Intelligence
- Force Microscopy Techniques and Applications
- Genetics, Aging, and Longevity in Model Organisms
- Sleep and Wakefulness Research
- Neuroscience and Neural Engineering
- Lipid Membrane Structure and Behavior
- Advanced Fluorescence Microscopy Techniques
- Advanced Memory and Neural Computing
- Physiological and biochemical adaptations
- Memory and Neural Mechanisms
- Fish Biology and Ecology Studies
- Molecular Communication and Nanonetworks
New Jersey Institute of Technology
2019-2024
Inserm
2012-2018
Sorbonne Université
2012-2018
Centre National de la Recherche Scientifique
2012-2018
Institut du Cerveau
2013-2018
Assistance Publique – Hôpitaux de Paris
2018
Northeastern University
2008-2014
Harvard University
2014
Harvard University Press
2013
Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Here we describe red, single-wavelength GECIs, "RCaMPs," engineered from circular permutation of the thermostable red fluorescent protein mRuby. High-resolution crystal structures mRuby, sensor RCaMP, and recently published GECI R-GECO1 give insight into chromophore environments Ca(2+)-bound state sensors domain interfaces different indicators. We characterized biophysical properties performance RCaMP...
The zebrafish larva stands out as an emergent model organism for translational studies involving gene or drug screening thanks to its size, genetics, and permeability. At the larval stage, locomotion occurs in short episodes punctuated by periods of rest. Although phenotyping behavior is a key component large-scale screens, it has not yet been automated this system. We developed ZebraZoom, program automatically track larvae identify maneuvers many animals performing discrete movements. Our...
Abstract Locomotion in vertebrates relies on motor circuits the spinal cord receiving inputs from hindbrain to execute commands while dynamically integrating proprioceptive sensory feedback. The spatial organization of neuronal networks driving locomotion and role inhibition has not been extensively investigated. Here, we mapped activity with single-cell resolution restrained transgenic Tg(HuC:GCaMP5G) zebrafish larvae swimming response whole-field visual motion. We combined large-scale...
Locomotion is an essential behaviour for the survival of all animals. The neural circuitry underlying locomotion therefore highly robust to a wide variety perturbations, including injury and abrupt changes in environment. In short term, fault tolerance networks allows persist immediately after mild moderate injury. longer many invertebrates vertebrates, reorganization anatomical regeneration can restore severe perturbations that initially caused paralysis. Despite decades research, very...
Mauthner cells are the largest neurons in hindbrain of teleost fish and most amphibians. Each cell has two major dendrites thought to receive segregated streams sensory input: lateral dendrite receives mechanosensory input while ventral visual input. These inputs, which mediate escape responses sudden stimuli, may be modulated by availability information animal. To understand impact absence on morphologies during developmental evolutionary time scales, we examined Astyanax mexicanus. This...
Collective behavior may be elicited or can spontaneously emerge by a combination of interactions with the physical environment and conspecifics moving within that environment. To investigate relative contributions these factors in small millimeter-scale swimming organism, we observed larval zebrafish, interacting at varying densities under circular confinement. If left undisturbed, zebrafish swim intermittently burst coast manner are socially independent this developmental stage, before...
Expansion Microscopy is a super-resolution technique in which physically enlarging samples an isotropic manner increases inter-molecular distances such that nano-scale structures can be resolved using light microscopy. This particularly useful neuroscience as many important are smaller than the diffraction limit. Since its invention 2015, variety of protocols have been generated and applied to advance knowledge prominent organisms neuroscience, including zebrafish, mice, Drosophila, C....
Abstract Mauthner cells are the largest neurons in hindbrain of teleost fish and most amphibians. Each cell has two major dendrites thought to receive segregated streams sensory input: lateral dendrite receives mechanosensory input while ventral visual input. These inputs, which mediate escape responses sudden stimuli, may be modulated by availability information animal. To understand impact absence on morphologies during development evolutionary time scales, we examined Astyanax mexicanus ....
ABSTRACT Collective behavior may be elicited or can spontaneously emerge by a combination of interactions with the physical environment and conspecifics moving within that environment. To investigate relative contributions these factors in small millimeter-scale swimming organism, we observed larval zebrafish, interacting at varying densities under circular confinement. Our aim was to understand biological mechanisms acting on larvae as they swim together inside confinements. If left...