A5086305320- Neurobiology and Insect Physiology Research
- Advanced Fluorescence Microscopy Techniques
- Neural dynamics and brain function
- Cellular Mechanics and Interactions
- Neuroscience and Neural Engineering
- CRISPR and Genetic Engineering
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Cell Image Analysis Techniques
- Plant and animal studies
- Caveolin-1 and cellular processes
- 3D Printing in Biomedical Research
- Zebrafish Biomedical Research Applications
- Lipid Membrane Structure and Behavior
- Retinal Development and Disorders
- CCD and CMOS Imaging Sensors
- Pluripotent Stem Cells Research
- Advanced Memory and Neural Computing
- Electrochemical Analysis and Applications
- Chromosomal and Genetic Variations
- Renal and related cancers
- RNA Interference and Gene Delivery
- Force Microscopy Techniques and Applications
- Genetics, Aging, and Longevity in Model Organisms
- Advanced biosensing and bioanalysis techniques
Technion – Israel Institute of Technology
2020-2025
Massachusetts Institute of Technology
2013-2020
McGovern Institute for Brain Research
2019-2020
Stanford University
2012-2017
Laboratoire d'Informatique de Paris-Nord
2017
Significance We explore the utility of expansion microscopy (ExM) in neuroscience and developmental biology using zebrafish model. Regarding studies, ExM enables tracing cellular processes brain, as well imaging synapses their biomolecular content organization. development, resolving nuclear compartments, particularly invaginations channels, helps relate such nanostructures to proteins cytoskeleton during embryogenesis.
The function of the brain is unlikely to be understood without an accurate description its output, yet nature movement elements and their organization remains open problem. Here, are identified from dynamics walking in flies, using unbiased criteria. On one time scale, consistent over hundreds milliseconds, allowing elementary features defined. Over longer periods, well described by a stochastic process composed these features, generative model this reproduces individual behavior sequences...
Abstract Brain organoids offer unprecedented insights into brain development and disease modeling hold promise for drug screening. Significant hindrances, however, are morphological cellular heterogeneity, inter-organoid size differences, stress, poor reproducibility. Here, we describe a method that reproducibly generates thousands of across multiple hiPSC lines. These High Quantity (Hi-Q organoids) exhibit reproducible cytoarchitecture, cell diversity, functionality, free from ectopically...
Determining the genomic locations of transposable elements is a common experimental goal. When mapping large collections transposon insertions, individualized amplification and sequencing both time consuming costly. We describe an approach in which numbers insertion lines can be simultaneously mapped single DNA reaction by using digital error-correcting codes to encode line identity unique set barcoded pools.
Rapid acquisition of large imaging volumes with microscopic resolution is an essential unmet need in biological research, especially for monitoring rapid dynamical processes such as fast activity distributed neural systems.
Expansion Microscopy is a super-resolution technique in which physically enlarging samples an isotropic manner increases inter-molecular distances such that nano-scale structures can be resolved using light microscopy. This particularly useful neuroscience as many important are smaller than the diffraction limit. Since its invention 2015, variety of protocols have been generated and applied to advance knowledge prominent organisms neuroscience, including zebrafish, mice, Drosophila, C....
Abstract Methods for one-photon fluorescent imaging of calcium dynamics in vivo are popular due to their ability simultaneously capture the hundreds neurons across large fields view, at a low equipment complexity and cost. In contrast two-photon methods, however, methods suffer from higher levels crosstalk between cell bodies surrounding neuropil, resulting decreased signal-to-noise artifactual correlations neural activity. Here, we address this problem by engineering body-targeted variants...
The Bursztyn et al. (2007) paper proposes a mathematical model of excitation-contraction in myometrial smooth muscle cell (SMC). incorporates processes intracellular Ca^2+ concentration control, myosin light chain (MLC) phosphorylation and stress production. We create modularized CellML implementation the model, which is able to simulate these against original data.
The Bursztyn et al. (2007) paper proposes a mathematical model of excitation-contraction in myometrial smooth muscle cell (SMC). incorporates processes intracellular Ca^2+ concentration control, myosin light chain (MLC) phosphorylation and stress production. We create modularized CellML implementation the model, which is able to simulate these against original data.