A5003565151- Neurobiology and Insect Physiology Research
- Retinal Development and Disorders
- Animal Behavior and Reproduction
- Neural dynamics and brain function
- Insect and Arachnid Ecology and Behavior
- Axon Guidance and Neuronal Signaling
- Circadian rhythm and melatonin
- Genetics, Aging, and Longevity in Model Organisms
- Developmental Biology and Gene Regulation
- Visual perception and processing mechanisms
- Zebrafish Biomedical Research Applications
- Photoreceptor and optogenetics research
- CRISPR and Genetic Engineering
- Physiological and biochemical adaptations
- Neuroscience and Neural Engineering
- Plant and Biological Electrophysiology Studies
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Insect behavior and control techniques
- Silk-based biomaterials and applications
- Plant and animal studies
- Viral Infectious Diseases and Gene Expression in Insects
- Spaceflight effects on biology
- Electrochemical Analysis and Applications
- Olfactory and Sensory Function Studies
Stanford University
2015-2024
Chan Zuckerberg Initiative (United States)
2024
Stanford Medicine
2021-2023
Fairchild Semiconductor (United States)
2023
Institute of Neurobiology
2011-2016
ORCID
2014
Howard Hughes Medical Institute
1995-2005
University of Oregon
2005
University of California, Los Angeles
1998-2003
California Institute of Technology
1995-1998
For more than 100 years, the fruit fly
Monitoring voltage dynamics in defined neurons deep the brain is critical for unraveling function of neuronal circuits but challenging due to limited performance existing tools. In particular, while genetically encoded indicators have shown promise optical detection transients, many exhibit low sensitivity when imaged under two-photon illumination. Previous studies thus fell short visualizing individual single trials. Here, we report ASAP2s, a novel indicator with improved sensitivity. By...
Genetically encoded voltage indicators are emerging tools for monitoring dynamics with cell-type specificity. However, current enable a narrow range of applications due to poor performance under two-photon microscopy, method choice deep-tissue recording. To improve indicators, we developed multiparameter high-throughput platform optimize microscopy. Using this system, identified JEDI-2P, an indicator that is faster, brighter, and more sensitive photostable than its predecessors. We...
Across animal phyla, motion vision relies on neurons that respond preferentially to stimuli moving in one, preferred direction over the opposite, null direction. In elementary detector of <i>Drosophila</i>, selectivity emerges two neuron types, T4 and T5, but computational algorithm underlying this remains unknown. We find receptive fields both T5 exhibit spatiotemporally offset light-preferring dark-preferring subfields, each obliquely oriented spacetime. a linear-nonlinear modeling...
Abstract Originally a genetic model organism, the experimental use of Drosophila melanogaster has grown to include quantitative behavioral analyses, sophisticated perturbations neuronal function, and detailed sensory physiology. A highlight these developments can be seen in context vision, where pioneering studies have uncovered fundamental generalizable principles processing. Here we begin with an overview vision-guided behaviors common methods for probing visual circuits. We then outline...