A5020082708- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Neurobiology and Insect Physiology Research
- Hippo pathway signaling and YAP/TAZ
- Wnt/β-catenin signaling in development and cancer
- Animal Behavior and Reproduction
- Cellular transport and secretion
- Epigenetics and DNA Methylation
- Insect behavior and control techniques
- Mosquito-borne diseases and control
- Ubiquitin and proteasome pathways
- Chromosomal and Genetic Variations
- Signaling Pathways in Disease
- CRISPR and Genetic Engineering
- Cellular Mechanics and Interactions
- Histone Deacetylase Inhibitors Research
- Developmental Biology and Gene Regulation
- Plant Molecular Biology Research
Philipps University of Marburg
2021-2023
University of California, San Francisco
2018-2022
Universidad Católica de Santa Fe
2019
University of Cologne
2018
Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases
2018
University of Göttingen
2018
For more than 100 years, the fruit fly
The Drosophila ovary is a widely used model for germ cell and somatic tissue biology. Here we use single-cell RNA-sequencing (scRNA-seq) to build comprehensive atlas of the adult that contains transcriptional profiles every major type in ovary, including germline stem cells their niche cells, follicle previously undescribed subpopulations escort cells. In addition, identify Gal4 lines with specific expression patterns perform lineage tracing We discover distinct subpopulation able convert...
Abstract The ability to obtain single cell transcriptomes for stable types and dynamic states is ushering in a new era biology. We created the Tabula Drosophilae , atlas of adult fruit fly which includes 580k cells from 15 individually dissected sexed tissues as well entire head body. Over 100 researchers community contributed annotations >250 distinct across all tissues. provide an in-depth analysis type-related gene signatures transcription factor markers, sexual dimorphism, whole...
Summary Controlled signaling activity is vital for normal tissue homeostasis and oncogenic activation facilitates tumorigenesis. Here we use single-cell transcriptomics to investigate the effects of pro-proliferative on epithelial using Drosophila follicle cell lineage. Notably, EGFR-Ras overactivation induces cycle defects by activating transcription factors Pointed E2f1 impedes differentiation. Hh simultaneously promotes an undifferentiated state differentiation via EMT-associated zfh1...
Abstract Changes in cell morphology require the dynamic remodeling of actin cytoskeleton. Calcium fluxes have been suggested as an important signal to rapidly relay information cytoskeleton, but underlying mechanisms remain poorly understood. Here, we identify EF-hand domain containing protein EFhD2/Swip-1 a conserved lamellipodial strongly upregulated Drosophila macrophages at onset metamorphosis when macrophage behavior shifts from quiescent migratory state. Loss- and gain-of-function...
Drosophila blood cells called hemocytes form an efficient barrier against infections and tissue damage. During metamorphosis, undergo tremendous changes in their shape behavior, preparing them for clearance. Yet, the diversity functional plasticity of pupal have not been explored. Here, we combine single-cell transcriptomics high-resolution microscopy to dissect heterogeneity hemocytes. We identified undifferentiated specified with different molecular signatures associated distinct functions...
Abstract Occluding junctions are essential for tissue integrity and barrier function in epithelial tissues. Here we identify several non-occluding functions of septate junction components, the invertebrate occluding junctions, follicle epithelium development. We show that morphogenesis follicular stalk is an actin-driven process requires intact apical-basal cell polarity, which controlled by components. Septate components further regulate signaling pathways a cell-type specific manner. In...
Abstract The Drosophila ovary is a widely used model for germ cell and somatic tissue biology. We have single-cell RNA-sequencing to build comprehensive atlas of the adult containing unique transcriptional profiles every major type in ovary, including germline follicle stem cells. Using this we identify novel tools identification manipulation known types perform lineage tracing test cellular relationships previously unknown types. By discovered new form plasticity which inner germarial...
Abstract Drosophila blood cells called hemocytes form an efficient barrier against infections and tissue damage. During metamorphosis, undergo tremendous changes in their shape behavior preparing them for clearance. Yet, the diversity functional plasticity of pupal have not been explored. Here, we combine single-cell transcriptomics high-resolution microscopy to dissect heterogeneity hemocytes. We identified precursor effector with distinct molecular signatures cellular functions clearly...