A5031292638- Invertebrate Immune Response Mechanisms
- Neurobiology and Insect Physiology Research
- Insect symbiosis and bacterial influences
- Cellular Mechanics and Interactions
- Genetics, Aging, and Longevity in Model Organisms
- Hippo pathway signaling and YAP/TAZ
- Wnt/β-catenin signaling in development and cancer
- Cancer Cells and Metastasis
- Insect Utilization and Effects
- Microtubule and mitosis dynamics
- Pluripotent Stem Cells Research
- Physiological and biochemical adaptations
- Insect behavior and control techniques
- 3D Printing in Biomedical Research
- Reflective Practices in Education
- Renal and related cancers
- Micro and Nano Robotics
- Digestive system and related health
- CRISPR and Genetic Engineering
- Liver physiology and pathology
- Developmental Biology and Gene Regulation
- Single-cell and spatial transcriptomics
- Polyamine Metabolism and Applications
- Ubiquitin and proteasome pathways
- Signaling Pathways in Disease
Stanford University
2013-2024
Chan Zuckerberg Initiative (United States)
2022-2023
Stanford Medicine
2021
University of California, Berkeley
2006-2011
University of California, San Francisco
2000-2010
Millennium Engineering and Integration (United States)
2010
Institute of Molecular Biology and Biophysics
2003
For more than 100 years, the fruit fly
Epithelial cells polarize and orient polarity in response to cell-cell cell-matrix adhesion. Although there has been much recent progress understanding the general polarizing machinery of epithelia, it is largely unclear how this controlled by extracellular environment. To explore signals from interactions that control orientation cell polarity, we have used three-dimensional culture systems which Madin-Darby canine kidney (MDCK) form polarized, lumen-containing structures. We show...
Abstract The ability to obtain single cell transcriptomes for stable types and dynamic states is ushering in a new era biology. We created the Tabula Drosophilae , atlas of adult fruit fly which includes 580k cells from 15 individually dissected sexed tissues as well entire head body. Over 100 researchers community contributed annotations >250 distinct across all tissues. provide an in-depth analysis type-related gene signatures transcription factor markers, sexual dimorphism, whole...
Epithelial cyst and tubule formation are critical processes that involve transient, highly choreographed changes in cell polarity. Factors controlling these polarity largely unknown. One candidate factor is the conserved eight-member protein complex called exocyst. We show during tubulogenesis an vitro model system exocyst relocalized along growing tubules consistent with In yeast, subunit Sec10p a crucial component linking polarized exocytic vesicles rest of and, ultimately, plasma...
Organ renewal is governed by the dynamics of cell division, differentiation and loss. To study these in real time, we present a platform for extended live imaging adult Drosophila midgut, premier genetic model stem-cell-based organs. A window cut into living animal allows midgut to be imaged while intact physiologically functioning. This approach prolongs sessions 12–16 hr yields movies that document tissue at vivid spatiotemporal resolution. By applying pipeline movie processing analysis,...
Epithelial cells form monolayers of polarized with apical and basolateral surfaces. Madin-Darby canine kidney epithelial transiently lose their apico-basolateral polarity become motile by treatment hepatocyte growth factor (HGF), which causes the monolayer to remodel into tubules. HGF induces produce extensions. Cells then migrate out chains cells, go on Herein, we have analyzed molecular mechanisms underlying production extensions chains. We find that switch from an polarization in...
Cell- and tissue-level processes often occur across days or weeks, but few imaging methods can capture such long timescales. Here, we describe Bellymount, a simple, noninvasive method for longitudinal of the Drosophila abdomen at subcellular resolution. Bellymounted animals remain live intact, so same individual be imaged serially to yield vivid time series multiday processes. This feature opens door studies internal organs in their native context. Exploiting Bellymount's capabilities, track...
Epithelial cells undergo striking morphological changes during division to ensure proper segregation of genetic and cytoplasmic materials. These occur despite dividing being mechanically restricted by neighboring cells, indicating the need for extracellular force generation. Beyond driving cell itself, forces associated with have been implicated in tissue-scale processes, including development, tissue growth, migration, epidermal stratification. While generated mitotic rounding are well...
In healthy adult organs, robust feedback mechanisms control cell turnover to enforce homeostatic equilibrium between division and death [1Pellettieri J. Sánchez Alvarado A. Cell tissue homeostasis: from humans planarians.Annu. Rev. Genet. 2007; 41: 83-105Crossref PubMed Scopus (209) Google Scholar, 2O'Brien L.E. Bilder D. Beyond the niche: tissue-level coordination of stem dynamics.Annu. Dev. Biol. 2013; 29: 107-136Crossref (40) Scholar]. Nascent tumors must subvert these achieve cancerous...
Proteasome inhibitor therapeutics (PITs) have the potential to cause peripheral neuropathy. In a mouse model of PIT-induced neuropathy, authors demonstrated that ubiquitin-positive multifocal protein aggregates with nuclear displacement appear in dorsal root ganglion cells animals subsequently develop nerve injuries. This peripheral-nerve effect nonclinical models has generally been recognized as correlate grade 3 neuropathy clinical testing. differentiated PC12 cells, perturbations...
Tubule formation in vitro using Madin-Darby canine kidney (MDCK) epithelial cells consists mainly of two processes. First, the undergo a partial epithelial–mesenchymal transition (pEMT), losing polarity and migrating. Second, redifferentiate, forming cords then tubules with continuous lumens. We have shown previously that extracellular signal-regulated kinase activation is required for pEMT. However, mechanism how pEMT phase turned off redifferentiation initiated largely unknown. To address...
Cytosolic Ca2+ is a highly dynamic, tightly regulated and broadly conserved cellular signal. dynamics have been studied widely in monocultures, yet organs vivo comprise heterogeneous populations of stem differentiated cells. Here, we examine the adult Drosophila intestine, self-renewing epithelial organ which cells continuously produce daughters that differentiate into either enteroendocrine or enterocytes. Live imaging whole ex reveals stem-cell adopt strikingly distinct patterns...