A5019266132- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Insect symbiosis and bacterial influences
- Genetics, Aging, and Longevity in Model Organisms
- Cellular transport and secretion
- CRISPR and Genetic Engineering
- Cellular Mechanics and Interactions
- Cancer-related gene regulation
- Microtubule and mitosis dynamics
- Advanced Chemical Sensor Technologies
- RNA regulation and disease
- Hippo pathway signaling and YAP/TAZ
- Sperm and Testicular Function
- Environmental DNA in Biodiversity Studies
- Epigenetics and DNA Methylation
- Contact Dermatitis and Allergies
- Insect behavior and control techniques
- Renal and related cancers
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Biotin and Related Studies
- Developmental Biology and Gene Regulation
- Invertebrate Immune Response Mechanisms
Stanford University
2018-2024
Stowers Institute for Medical Research
2023
For more than 100 years, the fruit fly
Proper differentiation of sperm from germline stem cells, essential for production the next generation, requires dramatic changes in gene expression that drive remodeling almost all cellular components, chromatin to organelles cell shape itself. Here, we provide a single nucleus and RNA-seq resource covering spermatogenesis Drosophila starting in-depth analysis adult testis (snRNA-seq) data Fly Cell Atlas (FCA) study. With over 44,000 nuclei 6000 cells analyzed, identification rare types,...
Abstract The ability to obtain single cell transcriptomes for stable types and dynamic states is ushering in a new era biology. We created the Tabula Drosophilae , atlas of adult fruit fly which includes 580k cells from 15 individually dissected sexed tissues as well entire head body. Over 100 researchers community contributed annotations >250 distinct across all tissues. provide an in-depth analysis type-related gene signatures transcription factor markers, sexual dimorphism, whole...
Tissue homeostasis and repair relies on proper communication of stem cells their differentiating daughters with the local tissue microenvironment. In Drosophila male germline adult cell lineage, germ proliferate progressively differentiate enclosed in supportive somatic cyst cells, forming a small organoid, functional unit differentiation. Here we show that polarity vesicle trafficking influence signal transduction profound effects they enclose. Our data suggest cortical components Dlg,...
Alternative polyadenylation (APA) generates transcript isoforms that differ in the position of 3′ cleavage site, resulting production mRNA with different length UTRs. Although widespread, role APA biology cells, tissues, and organisms has been controversial. We identified >500 Drosophila genes express a long UTR proliferating spermatogonia but short differentiating spermatocytes due to APA. show stage-specific choice end site can be regulated by arrangement canonical signal (PAS) near...
Abstract In adult stem cell lineages, the cellular microenvironment plays essential roles to ensure proper balance of self-renewal, differentiation and regulated elimination differentiating cells. Although death progenitor cells undergoing proliferation or early is a feature many tissues, mechanisms that initiate this pruning remain unexplored, particularly in male germline, where up 30% germline eliminated before meiotic divisions. We conducted targeted screen identify functional regulators...
Abstract Proper differentiation of sperm from germline stem cells, essential for production the next generation, requires dramatic changes in gene expression that drive remodeling almost all cellular components, chromatin to organelles cell shape itself. Here we provide a single nucleus and RNA-seq resource covering spermatogenesis Drosophila starting in-depth analysis adult testis (snRNA-seq) data Fly Cell Atlas (FCA) study (Li et al ., 2022). With over 44,000 nuclei 6,000 cells analyzed,...
SUMMARY Tissue homeostasis and repair relies on proper communication of stem cells their differentiating daughters with the local tissue microenvironment. In Drosophila male germline adult cell lineage, germ proliferate progressively differentiate enclosed in supportive somatic cyst cells, forming a small organoid, functional unit differentiation. Here we show that polarity vesicle trafficking influence signal transduction profound effects they enclose. Our data suggest both cortical...
It is a truism to say that most of our interaction with the environment depend on surface contacts one kind or another. The nature many interactions any individual his her skin, and thus we come critical argument against adopting Euro. The amount nickel regulated in products into prolonged repeated contact because allergies produced. One- two-euro coins induce positive skin-test reactions sensitive individuals, remarkably, release 240–320 times more than allowed European Union Nickel...
Abstract Alternative polyadenylation (APA) generates transcript isoforms that differ in the position of 3’ cleavage site, resulting production mRNA with different length 3’UTRs. Although widespread, role APA biology cells, tissues and organisms has been controversial. We identified over 500 Drosophila genes express a long 3’UTR proliferating spermatogonia but short differentiating spermatocytes due to APA. show stage-specific choice end site can be regulated by arrangement canonical signal...