A5018784559- Single-cell and spatial transcriptomics
- Neurobiology and Insect Physiology Research
- Cell Image Analysis Techniques
- CRISPR and Genetic Engineering
- Olfactory and Sensory Function Studies
- Genetics, Aging, and Longevity in Model Organisms
- Retinal Development and Disorders
- Invertebrate Immune Response Mechanisms
- Pluripotent Stem Cells Research
- Microbial Community Ecology and Physiology
- Alzheimer's disease research and treatments
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Insect symbiosis and bacterial influences
- Physiological and biochemical adaptations
- Cancer Genomics and Diagnostics
- Parkinson's Disease Mechanisms and Treatments
- Glycosylation and Glycoproteins Research
- 3D Printing in Biomedical Research
- Advanced Fluorescence Microscopy Techniques
- RNA regulation and disease
- Genomics and Phylogenetic Studies
- interferon and immune responses
VIB-KU Leuven Center for Brain & Disease Research
2017-2025
KU Leuven
2017-2025
Allen Institute for Brain Science
2024-2025
VIB-KU Leuven Center for Cancer Biology
2021-2025
ETH Zurich
2023-2024
Université Libre de Bruxelles
1979
The diversity of cell types and regulatory states in the brain, how these change during aging, remains largely unknown. We present a single-cell transcriptome atlas entire adult Drosophila melanogaster brain sampled across its lifespan. Cell clustering identified 87 initial clusters that are further subclustered validated by targeted cell-sorting. Our data show high granularity identify wide range types. Gene network analyses using SCENIC revealed heterogeneity linked to energy consumption....
For more than 100 years, the fruit fly
Proper differentiation of sperm from germline stem cells, essential for production the next generation, requires dramatic changes in gene expression that drive remodeling almost all cellular components, chromatin to organelles cell shape itself. Here, we provide a single nucleus and RNA-seq resource covering spermatogenesis Drosophila starting in-depth analysis adult testis (snRNA-seq) data Fly Cell Atlas (FCA) study. With over 44,000 nuclei 6000 cells analyzed, identification rare types,...
Abstract The ability to obtain single cell transcriptomes for stable types and dynamic states is ushering in a new era biology. We created the Tabula Drosophilae , atlas of adult fruit fly which includes 580k cells from 15 individually dissected sexed tissues as well entire head body. Over 100 researchers community contributed annotations >250 distinct across all tissues. provide an in-depth analysis type-related gene signatures transcription factor markers, sexual dimorphism, whole...
Single-cell RNA-seq and single-cell assay for transposase-accessible chromatin (ATAC-seq) technologies are used extensively to create cell type atlases a wide range of organisms, tissues, disease processes. To increase the scale these atlases, lower cost pave way more specialized multiome assays, custom droplet microfluidics may provide solutions complementary commercial setups. We developed HyDrop, flexible open-source microfluidic platform encompassing three protocols. The first protocol...
Pathogenic α-synuclein and tau are critical drivers of neurodegeneration, their mutations cause neuronal loss in patients. Whether the underlying preferential vulnerability is a cell-type-intrinsic property or consequence increased expression levels remains elusive. Here, we explore cell-type-specific human brain datasets use deep phenotyping as well brain-wide single-cell RNA sequencing >200 live neuron types fruit flies to determine which cellular environments react most toxicity. We...
In multipolar vertebrate neurons, action potentials (APs) initiate close to the soma, at axonal initial segment. Invertebrate neurons are typically unipolar with dendrites integrating directly into axon. Where APs initiated in axons of invertebrate is unclear. Voltage-gated sodium (Na V ) channels a functional hallmark segment vertebrates. We used an intronic Minos -Mediated Integration Cassette determine endogenous gene expression and subcellular localization sole Na channel both male...
Morphogens, secreted signalling molecules that direct cell fate and tissue development, are used to neuroepithelial progenitors towards discrete regional identities across the central nervous system. Neural tissues derived from pluripotent stem cells in vitro (neural organoids) provide new models for studying neural regionalization, however, we lack a comprehensive survey of how developing human neuroepithelium responds morphogen cues. Here, produce detailed map morphogen-induced effects on...
The classical diagnosis of Parkinsonism is based on motor symptoms that are the consequence nigrostriatal pathway dysfunction and reduced dopaminergic output. However, a decade prior to emergence issues, patients frequently experience non-motor symptoms, such as sense smell (hyposmia). cellular molecular bases for these early defects remain enigmatic. To explore this, we developed new collection five fruit fly models representing various forms familial conducted single-cell RNA sequencing...
The classical diagnosis of Parkinsonism is based on motor symptoms that are the consequence nigrostriatal pathway dysfunction and reduced dopaminergic output. However, a decade prior to emergence issues, patients frequently experience non-motor symptoms, such as sense smell (hyposmia). cellular molecular bases for these early defects remain enigmatic. To explore this, we developed new collection five fruit fly models familial conducted single-cell RNA sequencing young brains models....
Recently, we have achieved a significant milestone with the creation of Fly Cell Atlas. This single-nuclei atlas encompasses entire fly, covering head and body, in addition to all major organs. catalogs many hundreds cell types, which annotated 250. Thus, large number clusters remain be fully characterized, particular brain. Furthermore, by applying sequencing, information about spatial location cells body possible subcellular localization mRNAs within these is lost. Spatial transcriptomics...
Recently, we have achieved a significant milestone with the creation of Fly Cell Atlas. This single-nuclei atlas encompasses entire fly, covering head and body, in addition to all major organs. catalogs many hundreds cell types, which annotated 250. Thus, large number clusters remain be fully characterized, particular brain. Furthermore, by applying sequencing, information about spatial location cells body possible subcellular localization mRNAs within these is lost. Spatial transcriptomics...
The classical diagnosis of Parkinsonism is based on motor symptoms that are the consequence nigrostriatal pathway dysfunction and reduced dopaminergic output. However, a decade prior to emergence issues, patients frequently experience non-motor symptoms, such as sense smell (hyposmia). cellular molecular bases for these early defects remain enigmatic. To explore this, we developed new collection five fruit fly models familial conducted single-cell RNA sequencing young brains models....
Abstract The classical diagnosis of Parkinsonism is based on motor symptoms that are the consequence nigrostriatal pathway dysfunction and reduced dopaminergic output. However, a decade prior to emergence issues, patients frequently experience non-motor symptoms, such as sense smell (hyposmia). cellular molecular bases for these early defects remain enigmatic. To explore this, we developed new collection five fruit fly models familial conducted single-cell RNA sequencing young brains models....
Summary The Drosophila brain is a work horse in neuroscience. Single-cell transcriptome analysis 1–5, 3D morphological classification 6 , and detailed EM mapping of the connectome 7–10 have revealed an immense diversity neuronal glial cell types that underlie wide array functional behavioral traits fruit fly. identities these are controlled by – still unknown gene regulatory networks (GRNs), involving combinations transcription factors bind to genomic enhancers regulate their target genes....
Recently, we have achieved a significant milestone with the creation of Fly Cell Atlas. This single-nuclei atlas encompasses entire fly, covering head and body, in addition to all major organs. catalogs many hundreds cell types, which annotated 250. Thus, large number clusters remain be fully characterized, particular brain. Furthermore, by applying sequencing, information about spatial location cells body possible subcellular localization mRNAs within these is lost. Spatial transcriptomics...
Recently, we have achieved a significant milestone with the creation of Fly Cell Atlas. This single-nuclei atlas encompasses entire fly, covering head and body, in addition to all major organs. catalogs hundreds thousands cell types, which annotated 250. still leaves many clusters be fully characterized, particular brain. Furthermore, sequencing, information about spatial location cells mRNAs within these is lost. Here, provide solution this problem. In proof concept study, applied...
The classical diagnosis of Parkinsonism is based on motor symptoms that are the consequence nigrostriatal pathway dysfunction and reduced dopaminergic output. However, a decade prior to emergence issues, patients frequently experience non-motor symptoms, such as sense smell (hyposmia). cellular molecular bases for these early defects remain enigmatic. To explore this, we developed new collection five fruit fly models familial conducted single-cell RNA sequencing young brains models....
Summary The diversity of cell types and regulatory states in the brain, how these change during ageing, remains largely unknown. Here, we present a single-cell transcriptome catalogue entire adult Drosophila melanogaster brain sampled across its lifespan. Both neurons glia age through process “regulatory erosion”, characterized by strong decline RNA content, accompanied increasing transcriptional chromatin noise. We identify more than 50 specific transcription factors their downstream gene...