A5030591646- Neurobiology and Insect Physiology Research
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Monoclonal and Polyclonal Antibodies Research
- Invertebrate Immune Response Mechanisms
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Cellular Mechanics and Interactions
- Signaling Pathways in Disease
- Proteoglycans and glycosaminoglycans research
- Insect behavior and control techniques
- Animal Behavior and Reproduction
- Tuberous Sclerosis Complex Research
- Insect symbiosis and bacterial influences
- Hippo pathway signaling and YAP/TAZ
- Cellular transport and secretion
- Transgenic Plants and Applications
- Olfactory and Sensory Function Studies
- Single-cell and spatial transcriptomics
- Genomics and Chromatin Dynamics
- Prostate Cancer Treatment and Research
- Gene Regulatory Network Analysis
- Protease and Inhibitor Mechanisms
- Aquaculture disease management and microbiota
- Plant Molecular Biology Research
Boston VA Research Institute
2021-2024
Harvard University
2021-2024
Kansas City University
2024
Howard Hughes Medical Institute
2021-2022
Stanford University
2022
The University of Queensland
2014-2019
For more than 100 years, the fruit fly
Expansion of the available repertoire reagents for visualization and manipulation proteins will help understand their function. Short epitope tags linked to interest recognized by existing binders such as nanobodies facilitate protein studies obviating need isolate new antibodies directed against them. Nanobodies have several advantages over conventional antibodies, they can be expressed used tools in vivo. Here, we characterize two short (<15aa) NanoTag epitopes, 127D01 VHH05, corresponding...
Abstract Paraneoplastic syndromes occur in cancer patients and originate from dysfunction of organs at a distance the tumor or its metastasis. A wide range can be affected paraneoplastic syndromes; however, pathological mechanisms by which tumors influence host are poorly understood. Recent studies fly uncovered that secreted factors target organs, leading to effects. In this study, using Drosophila gut model, we characterize mechanism tumor-induced kidney dysfunction. Specifically, find...
Abstract Multicellular organisms rely on cell–cell communication to exchange information necessary for developmental processes and metabolic homeostasis. Cell–cell pathways can be inferred from transcriptomic datasets based ligand–receptor expression. Recently, data generated single-cell RNA sequencing have enabled interaction predictions at an unprecedented resolution. While computational methods are available infer in vertebrates such a tool does not yet exist Drosophila. Here, we...
Alternative splicing of Drosophila Dscam2 is regulated by cell type–specific expression the RNA binding protein Muscleblind.
How the brain makes trillions of synaptic connections using a genome only 20,000 genes is major question in modern neuroscience. Alternative splicing one mechanism that can increase number proteins produced by each gene, but its role regulating synapse formation poorly understood. In Drosophila, photoreceptors form with multiple postsynaptic elements including lamina neurons L1 and L2. L2 express distinct isoforms homophilic repulsive protein Dscam2, since these cannot bind to other,...
Alternative splicing (AS) allows a single gene to generate multiple protein isoforms. It has been hypothesized that AS plays role in brain wiring by increasing the number of cell recognition molecules necessary for forming connections between neurons. Many studies have characterized isoform expression patterns various genes brain, but very few addressed whether specific isoforms play functional neuronal wiring. In our recent work, we reported cell-type-specific molecule Dscam2. Exclusive...
Summary The explosive growth of regulatory hypotheses from single-cell datasets demands accurate prioritization for in vivo validation. However, current computational methods emphasize overall accuracy network reconstruction rather than prioritizing a limited set causal transcription factors (TFs) that can be feasibly tested. We developed Haystack, hybrid computational-biological algorithm combines active learning and the concept optimal transport theory to nominate validate high-confidence...
Abstract Paraneoplastic syndromes occur in cancer patients and originate from dysfunction of organs at a distance the tumor or its metastasis. A wide range can be affected paraneoplastic syndromes; however, pathological mechanisms by which tumors influence host are poorly understood. Recent studies fly uncovered that secreted factors target organs, leading to effects. In this study, using Drosophila gut model, we characterized mechanism tumor-induced kidney dysfunction. Specifically, found...
The sense of taste is essential for survival, as it allows animals to distinguish between foods that are nutritious from those toxic. However, innate responses different tastants can be modulated or even reversed under pathological conditions. Here, we examined whether and how the internal status an animal impacts valence by using Drosophila models hyperproliferation in gut. In all three where expressed proliferation-inducing transgenes intestinal stem cells (ISCs), ISCs caused a tumor-like...
Selective androgen receptor modulators (SARMs) have gained popularity for their alleged ability to selectively target receptors, potentially offering muscle-building benefits with fewer side effects than traditional steroids. However, the safety profile of SARMs, including RAD-140, is not fully understood. This case report presents a 29-year-old male who developed liver injury after taking RAD-140. The patient experienced jaundice and elevated enzymes three months RAD-140 use. A ultrasound...
ABSTRACT The brain extracellular matrix (ECM) is enriched in chondroitin sulphate proteoglycans (CSPGs) with variable modifications that intimately participate maturation and function. Very little known about how the changing biophysical properties of CSPGs are signalled to neurons. Here, we report Fibrinogen C Domain Containing 1 (FIBCD1), a chitin-binding receptor innate immune system, be highly expressed hippocampus specifically bind containing 4-O modification (CS-4S). Cultured Fibcd1...
ABSTRACT Multicellular organisms rely on cell-cell communication to exchange information necessary for developmental processes and metabolic homeostasis. Cell-cell pathways can be inferred from transcriptomic datasets based ligand-receptor (L-R) expression. Recently, data generated single cell RNA sequencing (scRNA-seq) have enabled L-R interaction predictions at an unprecedented resolution. While computational methods are available infer in vertebrates such a tool does not yet exist...
Abstract Expansion of the available repertoire reagents for visualization and manipulation proteins will help understand their function. Short epitope tags linked to interest recognized by existing binders such as nanobodies facilitate protein studies obviating need isolate new antibodies directed against them. Nanobodies have several advantages over conventional antibodies, they can be expressed used tools in vivo . Here, we characterize two short (<15 aa) NanoTag epitopes, 127D01 VHH05)...
Summary Alternative splicing of genes increases the number distinct proteins in a cell. In brain it is highly prevalent, presumably because proteome diversity crucial for establishing complex circuitry between trillions neurons. To provide individual cells with different repertoires protein isoforms, however, this process must be regulated. Previously, we found that mutually exclusive alternative cell surface protein, Dscam2 produces two isoforms (exon 10A and 10B) unique binding properties....
Expansion of the available repertoire reagents for visualization and manipulation proteins will help understand their function. Short epitope tags installed on interest recognized by existing binders such as nanobodies facilitate protein studies obviating need to isolate new antibodies directed against them. Nanobodies have several advantages over conventional antibodies, they can be expressed used tools in vivo. Here, we combine short epitopes (NanoTags) specific them characterizing two...