A5018280421- Developmental Biology and Gene Regulation
- Neurobiology and Insect Physiology Research
- Ubiquitin and proteasome pathways
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Invertebrate Immune Response Mechanisms
- Microtubule and mitosis dynamics
- Physiological and biochemical adaptations
- RNA Research and Splicing
- Genetics, Aging, and Longevity in Model Organisms
- Insect symbiosis and bacterial influences
- CRISPR and Genetic Engineering
- Protist diversity and phylogeny
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Nuclear Structure and Function
- Marine Invertebrate Physiology and Ecology
- Hippo pathway signaling and YAP/TAZ
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Advanced Fluorescence Microscopy Techniques
- Signaling Pathways in Disease
- Axon Guidance and Neuronal Signaling
- Lipid metabolism and biosynthesis
- Amino Acid Enzymes and Metabolism
- Congenital heart defects research
Centre National de la Recherche Scientifique
2009-2025
Institut de Biologie du Développement Marseille
2007-2025
Aix-Marseille Université
2014-2025
Institut de Biologie Valrose
2019-2025
Inserm
2022-2024
Université Côte d'Azur
2022-2024
Imperial College London
2014-2021
MRC London Institute of Medical Sciences
2017-2021
Hammersmith Hospital
2014-2019
Institut de Génomique Fonctionnelle de Lyon
2012
For more than 100 years, the fruit fly
Physiology and metabolism are often sexually dimorphic, but the underlying mechanisms remain incompletely understood. Here, we use intestine of Drosophila melanogaster to investigate how gut-derived signals contribute sex differences in whole-body physiology. We find that carbohydrate handling is male-biased a specific portion intestine. In contrast known sexual dimorphisms invertebrates, intestinal extrinsically controlled by adjacent male gonad, which activates JAK-STAT signaling...
Highlights•The Drosophila tracheal system exhibits nutrient-dependent plasticity•Tracheal plasticity is organ specific and metabolically significant•Nutrient- hypoxia-responsive neurons drive adaptive tracheation•Distinct insulin-like Pdf neuropeptides control organ-specific branchingSummaryDuring angiogenesis, a key process in the etiology treatment of cancer obesity, vasculature changes to meet metabolic needs its target tissues. Although cues governing vascular remodeling are not fully...
Hox transcription factors are essential for shaping body morphology in development and evolution. The control of protein activity part arises from interaction with the PBC class partners, pre-B cell factor (Pbx) proteins vertebrates Extradenticle (Exd) Drosophila. Characterized interactions occur through a single mode, involving short hexapeptide motif protein. This apparent uniqueness Hox-PBC provides little mechanistic insight how same cofactors endow specific diverse activities. Here, we...
Abstract Background Protein interactions control the regulatory networks underlying developmental processes. The understanding of complexity will, therefore, require characterization protein within their proper environment. bimolecular fluorescence complementation (BiFC) technology offers this possibility as it enables direct visualization in living cells. However, its potential has rarely been applied embryos animal model organisms and was only performed under transient expression levels....
Hox transcription factors control a number of developmental processes with the help PBC class proteins. In vitro analyses have established that formation Hox/PBC complexes relies on short conserved protein motif called hexapeptide (HX). This paradigm is at basis vast majority experimental approaches dedicated to study function. Here we questioned unique and general use HX for recruitment by using Bimolecular Fluorescence Complementation (BiFC) assay. method allows analyzing Hox-PBC...
Despite tremendous body form diversity in nature, bilaterian animals share common sets of developmental genes that display conserved expression patterns the embryo. Among them are Hox genes, which define different identities along anterior–posterior axis. proteins exert their function by interaction with TALE transcription factors. and members also present some but not all non-bilaterian phyla, raising question how Hox–TALE interactions evolved to provide positional information. By using...
Hox proteins are well-established developmental regulators that coordinate cell fate and morphogenesis throughout embryogenesis. In contrast, our knowledge of their specific molecular modes action is limited to the interaction with few cofactors. Here, we show able interact a wide range transcription factors in live Drosophila embryo. this context, specificity relies on versatile usage conserved short linear motifs (SLiMs), which, surprisingly, often restrains potential proteins. This novel...
Nutrient-dependent body size plasticity differs between the sexes in most species, including mammals. Previous work Drosophila showed that was higher females, yet mechanisms underlying increased female remain unclear. Here, we discover a protein-rich diet augments females and not males because of female-biased increase activity conserved insulin/insulin-like growth factor signaling pathway (IIS). This sex-biased upregulation IIS triggered by diet-induced stunted mRNA required insulin-like...
Heterochromatin protein 1 (HP1) family proteins have a well-characterized role in heterochromatin packaging and gene regulation. Their function organismal development, however, is less well understood. Here we used genome-wide expression profiling to assess novel functions of the Caenorhabditis elegans HP1 homolog HPL-2 at specific developmental stages.We show that regulates germline genes, extracellular matrix components genes involved lipid metabolism. Comparison our data with ChIP-on-chip...
Hox genes in species across the metazoa encode transcription factors (TFs) containing highly-conserved homeodomains that bind target DNA sequences to regulate batteries of developmental genes. DNA-bound proteins, together with other TF partners, induce an appropriate transcriptional response by RNA Polymerase II (PolII) and its associated general factors. How evolutionarily conserved TFs interface this machinery generate finely regulated responses remains obscure. One major component PolII...
Reproduction in sexually dimorphic animals relies on successful gamete production, executed by the germline and aided somatic support cells. Somatic sex identity Drosophila is instructed sex-specific isoforms of DMRT1 ortholog Doublesex (Dsx). Female-specific expression Sex-lethal (Sxl) causes alternative splicing transformer (tra) to female isoform traF. In turn, TraF alternatively splices dsx dsxF. Loss transcriptional repressor Chinmo male stem cells (CySCs) testis them "feminize",...
Abstract While sex chromosomes carry sex-determining genes, they also often differ from autosomes in size and composition, consisting mainly of silenced heterochromatic repetitive DNA. Even though Y show structural heteromorphism, the functional significance such differences remains elusive. Correlative studies suggest that amount chromosome heterochromatin might be responsible for several male-specific traits, including sex-specific longevity observed across a wide spectrum species, humans....
Hox proteins, a sub-group of the homeodomain (HD) transcription factor family, provide positional information for axial patterning in development and evolution. protein functional specificity is reached, at least part, through interactions with Pbc (Extradenticle (Exd) Drosophila ) Meis/Prep (Homothorax (Hth) proteins. Most our current knowledge stems from study anterior central identifying molecular structural bases Hox/Pbc/Meis-Prep cooperative action. Posterior class Abdominal-B (Abd-B)...
Abstract Development of the mammalian heart is mediated by complex interactions between myocardial, endocardial, and neural crest‐derived cells. Studies in Drosophila have shown that Slit‐Robo signaling pathway controls cardiac cell shape changes lumen formation tube. Here, we demonstrate situ hybridization multiple Slit ligands Robo receptors are expressed developing mouse heart. Slit3 predominant ligand transcribed early ventral wall linear tube subsequently chamber but not...
Expression of the Ret receptor tyrosine kinase is a defining feature enteric neurons. Its importance underscored by effects its mutation in Hirschsprung disease, leading to absence gut innervation and severe gastrointestinal symptoms. We report new physiologically significant site expression intestine: intestinal epithelium. Experiments Drosophila indicate that expressed both neurons adult epithelial progenitors, which require sustain their proliferation. Mechanistically, engaged positive...
Abstract The molecular mechanisms connecting cellular metabolism with differentiation remain poorly understood. Here, we find that metabolic signals contribute to stem cell and germline homeostasis during Drosophila melanogaster spermatogenesis. We discovered external citrate, originating outside the gonad, fuels production of Acetyl-coenzyme A by ATP-citrate lyase (dACLY). show this pathway is essential final spermatogenic stages, where a high level promotes NatB-dependent N-terminal...
Abstract Homochirality is a fundamental feature of all known forms life, maintaining biomolecules (amino-acids, proteins, sugars, nucleic acids) in one specific chiral form. While this condition central to biology, the mechanisms by which adverse accumulation non- l- α-amino-acids proteins lead pathophysiological consequences remain poorly understood. To address how heterochirality build-up impacts organism’s health, we use chiral-selective vivo assays detect protein-bound non -l -α-amino...
The emergence following gene duplication of a large repertoire Hox paralogue proteins underlies the importance taken by in controlling animal body plans development and evolution. Sequence divergence paralogous accounts for functional specialization, promoting axial morphological diversification bilaterian animals. Yet functionally specialized also continue performing ancient common functions. In this study, we investigate how highly divergent perform an identical function. This was achieved...
Abstract Editor's suggested further reading in BioEssays ftz Evolution: Findings, hypotheses and speculations (response to DOI 10.1002/bies.201100019 ) On the border of homeotic function: Re‐evaluating controversial role cofactor‐recruiting motifs Control DNA replication: A new facet Hox proteins? Classification sequence signatures: a guide protein function