Ruei‐Jiun Hung

ORCID: 0000-0002-0770-8031
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About
Contact & Profiles
Research Areas
  • Invertebrate Immune Response Mechanisms
  • Cellular Mechanics and Interactions
  • Insect symbiosis and bacterial influences
  • Advanced Fluorescence Microscopy Techniques
  • Aquaculture disease management and microbiota
  • CRISPR and Genetic Engineering
  • Genetics, Aging, and Longevity in Model Organisms
  • Axon Guidance and Neuronal Signaling
  • Zebrafish Biomedical Research Applications
  • Cellular transport and secretion
  • Neurobiology and Insect Physiology Research
  • Cardiomyopathy and Myosin Studies
  • Renal and related cancers
  • Physiological and biochemical adaptations
  • Angiogenesis and VEGF in Cancer
  • Insect behavior and control techniques
  • Wnt/β-catenin signaling in development and cancer
  • Marine Biology and Environmental Chemistry
  • Enzyme Structure and Function
  • Cancer, Hypoxia, and Metabolism
  • Redox biology and oxidative stress
  • Hippo pathway signaling and YAP/TAZ
  • Microfluidic and Capillary Electrophoresis Applications
  • Mosquito-borne diseases and control
  • Glycosylation and Glycoproteins Research

Harvard University
2016-2022

Boston VA Research Institute
2019-2022

The University of Texas Southwestern Medical Center
2010-2021

Southwestern Medical Center
2016

National Tsing Hua University
2007-2010

Georgetown University Medical Center
2004

Georgetown University
2004

Hongjie Li Jasper Janssens Maxime De Waegeneer Sai Saroja Kolluru Kristofer Davie and 95 more Vincent Gardeux Wouter Saelens Fabrice David Maria Brbić Katina I. Spanier Jure Leskovec Colleen N. McLaughlin Qijing Xie Robert C. Jones Katja Brueckner Jiwon Shim Sudhir Gopal Tattikota Frank Schnorrer Katja Rust Todd Nystul Zita Carvalho-Santos Carlos Ribeiro Soumitra Pal Sharvani Mahadevaraju Teresa M. Przytycka Aaron M. Allen Stephen F. Goodwin Cameron W. Berry Margaret T. Fuller Helen White‐Cooper Erika Matunis Stephen DiNardo Anthony Galenza Lucy Erin O’Brien Julian A. T. Dow Heinrich Jasper Brian Oliver Norbert Perrimon Bart Deplancke Stephen R. Quake Liqun Luo Stein Aerts Devika Agarwal Yasir H. Ahmed-Braimah Michelle N Arbeitman Majd Ariss Jordan Augsburger Kumar Ayush Catherine C. Baker Torsten U. Banisch Katja Birker Rolf Bodmer Benjamin Bolival Susanna E. Brantley Julie A. Brill Nora C. Brown Norene A. Buehner Xiaoyu Cai Rita Cardoso-Figueiredo Fernando Casares Amy K. Chang Thomas R. Clandinin Sheela Crasta Claude Desplan Angela M. Detweiler Darshan B. Dhakan Erika Donà Stefanie Engert Swann Floc’hlay Nancy George Amanda J. González-Segarra Andrew K. Groves Samantha C. Gumbin Yanmeng Guo D. Harris Yael Heifetz Stephen L. Holtz Felix Horns Bruno Hudry Ruei‐Jiun Hung Yuh Nung Jan Jacob S Jaszczak Gregory S.X.E. Jefferis Jim Karkanias Timothy L. Karr Nadja Sandra Katheder James Kezos Anna Kim Seung K. Kim Lutz Kockel Νικόλαος Κωνσταντινίδης Thomas B. Kornberg Henry M. Krause Andrew Thomas Labott Meghan Laturney Ruth Lehmann Sarah G. Leinwand Jun Li Joshua Shing Shun Li Kai Li

For more than 100 years, the fruit fly

10.1126/science.abk2432 article EN Science 2022-03-03

A protein involved in redox signaling disassembles actin filaments and alters their reassembly.

10.1126/science.1211956 article EN Science 2011-11-25

Studies of the adult Drosophila midgut have led to many insights in our understanding cell-type diversity, stem cell regeneration, tissue homeostasis, and fate decision. Advances single-cell RNA sequencing provide opportunities identify new types molecular features. We used characterize transcriptome epithelial cells identified 22 distinct clusters representing intestinal cells, enteroblasts, enteroendocrine (EEs), enterocytes. This unbiased approach recovered most known cells/enteroblast EE...

10.1073/pnas.1916820117 article EN Proceedings of the National Academy of Sciences 2020-01-08

blood cells, called hemocytes, are classified into plasmatocytes, crystal and lamellocytes based on the expression of a few marker genes cell morphologies, which inadequate to classify complete hemocyte repertoire. Here, we used single-cell RNA sequencing (scRNA-seq) map hemocytes across different inflammatory conditions in larvae. We resolved plasmatocytes states involved cycle, antimicrobial response, metabolism together with identification intermediate states. Further, discovered rare...

10.7554/elife.54818 article EN cc-by eLife 2020-05-12

Recent advances in single-cell sequencing provide a unique opportunity to gain novel insights into the diversity, lineage, and functions of cell types constituting tissue/organ. Here, we performed single-nucleus study adult Drosophila renal system, consisting Malpighian tubules nephrocytes, which shares similarities with mammalian kidney. We identified 11 distinct clusters representing stem cells, stellate regionally specific principal garland nephrocyte pericardial nephrocytes....

10.1073/pnas.2203179119 article EN cc-by Proceedings of the National Academy of Sciences 2022-06-13

Hippo signaling and the activity of its transcriptional coactivator, Yorkie (Yki), are conserved crucial regulators tissue homeostasis. In Drosophila midgut, after damage, Yki increases to stimulate stem cell proliferation, but how is turned off once repaired unknown. From an RNAi screen, we identified septate junction (SJ) protein tetraspanin 2A (Tsp2A) as a tumor suppressor. Tsp2A undergoes internalization facilitate endocytic degradation atypical kinase C (aPKC), negative regulator...

10.1016/j.celrep.2018.12.079 article EN cc-by-nc-nd Cell Reports 2019-01-01

Sphingosine 1-phosphate (S1P), a bioactive phospholipid, simultaneously induces actin cytoskeletal rearrangements and activation of matriptase, membrane-associated serine protease in human mammary epithelial cells. In this study, we used monoclonal antibody selective for activated, two-chain matriptase to examine the functional relationship between these two S1P-induced events. Ten minutes after exposure 184 A1N4 cells S1P, was observed accumulate at cell-cell contacts. Activated first began...

10.1152/ajpcell.00400.2003 article EN AJP Cell Physiology 2004-04-01

UDP-glucose dehydrogenase (UGDH) catalyzes a two-step NAD(+)-dependent oxidation of to produce UDP-glucuronic acid, which is common substrate for the biosynthesis exopolysaccharide. Searching Pseudomonas aeruginosa PAO1 genome data base UGDH has helped identify two open reading frames, PA2022 and PA3559, may encode UGDH. To elucidate their enzymatic identity, genes were cloned overexpressed in Escherichia coli, recombinant proteins purified. Both gene products are active as dimers capable...

10.1074/jbc.m701824200 article EN cc-by Journal of Biological Chemistry 2007-04-19

Many tumor cells are capable of migrating through endothelial cell (EC) junctions and disintegrating sub-endothelial extracellular matrix to achieve extravasation. We demonstrate in this study that certain solid can induce EC apoptosis facilitate their escape from the circulation. The is triggered by elevated intracellular reactive oxygen species (ROS) levels direct contacts with required. Treating ECs antioxidants, such as ascorbate N-acetyl-L-cysteine (NAC), a glutathione precursor rescue...

10.1002/jcp.22504 article EN Journal of Cellular Physiology 2010-11-10

Summary Drosophila blood cells, called hemocytes, are classified into plasmatocytes, crystal and lamellocytes based on the expression of a few marker genes cell morphologies, which inadequate to classify complete hemocyte repertoire. Here, we used single-cell RNA sequencing (scRNA-seq) map hemocytes across different inflammatory conditions in larvae. We resolved plasmatocytes states involved cycle, antimicrobial response, metabolism together with identification intermediate states. Further,...

10.1101/2019.12.20.884999 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2019-12-21

Studies on the adult Drosophila midgut have led many insights our understanding of cell type diversity, stem regeneration, tissue homeostasis and fate decision. Advances in scRNA-seq provide opportunities to identify new types molecular features. We used inDrop characterize transcriptome epithelial cells identified 12 distinct clusters representing intestinal (ISCs), enteroblasts (EBs), enteroendocrine (EEs), enterocytes (ECs). This unbiased approach recovered 90% known ISCs/EBs markers...

10.2139/ssrn.3259138 article EN SSRN Electronic Journal 2018-01-01

Abstract Studies of the adult Drosophila midgut have provided a number insights on cell type diversity, stem regeneration, tissue homeostasis and fate decision. Advances in single-cell RNA sequencing (scRNA-seq) provide opportunities to identify new types molecular features. We used inDrop characterize transcriptome epithelial cells identified 12 distinct clusters representing intestinal (ISCs), enteroblasts (EBs), enteroendocrine (EEs), enterocytes (ECs) from different regions, cardia. This...

10.1101/410423 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2018-09-08

SUMMARY Like humans, insects rely on precise regulation of their internal environments to survive. The insect renal system consists Malpighian tubules and nephrocytes that share similarities the mammalian kidney. Studies Drosophila have provided many insights into our understanding excretion waste products, stem cell regeneration, protein reabsorption, as human kidney disease models. Here, we analyzed single-nucleus RNA sequencing (snRNA-seq) data sets characterize types adult fly We...

10.1101/2021.09.03.458871 preprint EN cc-by-nc-nd bioRxiv (Cold Spring Harbor Laboratory) 2021-09-04

To change their behaviors, cells require actin proteins to assemble together into long polymers/filaments-and so a critical goal is understand the factors that control this filament (F-actin) assembly and stability. We have identified family of unusual regulators, MICALs, which are flavoprotein monooxygenase/hydroxylase enzymes associate with flavin adenine dinucleotide (FAD) use co-enzyme nicotinamide phosphate (NADPH) in Redox reactions. F-actin specific substrate for these MICAL enzymes,...

10.3390/ijms22041991 article EN International Journal of Molecular Sciences 2021-02-17

Like humans, insects rely on precise regulation of their internal environments to survive. The insect renal system consists Malpighian tubules and nephrocytes that share similarities the mammalian kidney. Studies Drosophila have provided many insights into our understanding excretion waste products, stem cell regeneration, protein reabsorption, as human kidney disease models. Here, we analyzed single-nucleus RNA sequencing (snRNA-seq) data sets characterize types adult fly We identified 11...

10.2139/ssrn.3932603 article EN SSRN Electronic Journal 2021-01-01
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