A5053132852- Insect symbiosis and bacterial influences
- Gut microbiota and health
- Evolution and Genetic Dynamics
- Invertebrate Immune Response Mechanisms
- Insect Utilization and Effects
- Genetic and Kidney Cyst Diseases
- Micro and Nano Robotics
- Plant and animal studies
- Evolutionary Game Theory and Cooperation
- Protist diversity and phylogeny
- Neurobiology and Insect Physiology Research
- Insect behavior and control techniques
- Genetics, Aging, and Longevity in Model Organisms
- Bioinformatics and Genomic Networks
- Gene Regulatory Network Analysis
- Genetic diversity and population structure
- Enzyme Production and Characterization
- Microbial Community Ecology and Physiology
- Cell Image Analysis Techniques
- Mosquito-borne diseases and control
- Forensic Entomology and Diptera Studies
- Studies on Chitinases and Chitosanases
- Genomics and Phylogenetic Studies
- Clostridium difficile and Clostridium perfringens research
- Insect and Pesticide Research
Carnegie Institution for Science
2017-2025
Department of Embryology
2017-2024
Johns Hopkins University
2018-2024
University of Baltimore
2024
Carnegie Observatories
2019-2023
University of California, Berkeley
2016-2022
University of California, San Francisco
2009-2019
Institut thématique Génétique, génomique et bioinformatique
2017
Stanford University
2004-2006
Pacific University
2004
Gut bacteria can affect key aspects of host fitness, such as development, fecundity, and lifespan, while the host, in turn, shapes gut microbiome. However, it is unclear to what extent individual species versus community interactions within microbiome are linked fitness. Here, we combinatorially dissect natural Drosophila melanogaster reveal that between shape fitness through life history tradeoffs. Empirically, made germ-free flies colonized with each possible combination five core fly...
Abstract The gut is continuously invaded by diverse bacteria from the diet and environment, yet microbiome composition relatively stable over time for host species ranging mammals to insects, suggesting host-specific factors may selectively maintain key of bacteria. To investigate specificity, we used gnotobiotic Drosophila , microbial pulse-chase protocols, microscopy stability different strains in fly gut. We show that a host-constructed physical niche foregut binds with strain-level...
The assembly and maintenance of eukaryotic flagella are regulated by intraflagellar transport (IFT), the bidirectional traffic IFT particles (recently renamed trains) within flagellum. We previously proposed balance-point length control model, which predicted that frequency train should decrease as a function flagellar length, thus modulating length-dependent rate. However, this model was challenged differential interference contrast microscopy observation is independent. Using total...
Cilia and flagella are microtubule-based organelles that protrude from the cell body. Ciliary assembly requires intraflagellar transport (IFT), a motile system delivers cargo body to flagellar tip for assembly. The process controlling injections of IFT proteins into compartment is, therefore, crucial ciliogenesis. Extensive biochemical genetic analyses have determined molecular machinery IFT, but these studies do not explain what regulates injection rate. Here, we provide evidence result...
Highlights•Microbial association promotes fly longevity and development on low-protein diet•A wide range of microbes can serve as a source protein during undernutrition•The extent effects correlates with microbiota quantity biomass•The most impactful microbial species simply thrive culture mediumSummaryIn Drosophila, promote or extend life. We tested the impact malnutrition show that is predictor longevity. Although all microbes, when abundantly provided, rescue lifespan diet, effect single...
Predicting antibiotic efficacy within microbial communities remains highly challenging. Interspecies interactions can impact activity through many mechanisms, including alterations to bacterial physiology. Here, we studied synthetic constructed from the core members of fruit fly gut microbiota. Co-culturing Lactobacillus plantarum with Acetobacter species altered its tolerance transcriptional inhibitor rifampin. By measuring key metabolites and environmental pH, determined that counter...
Observational studies reveal substantial variability in microbiome composition across individuals. Targeted gnotobiotic animals underscore this by showing that some bacterial strains colonize deterministically, while others stochastically. While of can be explained external factors like environmental, dietary, and genetic differences between individuals, paper we show for the model organism Drosophila melanogaster, interactions bacteria affect assembly process, contributing to a baseline...
The animal foregut is the first tissue to encounter ingested food, bacteria, and viruses. We characterized adult Drosophila using transcriptomics better understand how it triages consumed items for digestion or immune response manages resources. Cell types were assigned validated GFP-tagged Gal4 reporter lines. Foregut-associated neuroendocrine cells play a major integrative role by coordinating gut activity with nutrition, microbiome, circadian cycles; some express clock genes. Multiple...
Gut bacteria are prevalent throughout the Metazoa and form complex microbial communities associated with food breakdown, nutrient provision disease prevention. How hosts acquire maintain a consistent bacterial flora remains mysterious even in best-studied animals, including humans, mice, fishes, squid, bugs, worms flies. This essay visits evidence that have co-evolved relationships specific some of these supported by specialized physical niches select, sequester symbionts. Genetics...
The commensal microbiome has been shown to protect against newly introduced enteric pathogens in multiple host species, a phenomenon known as priority effect. Multiple mechanisms can contribute this protective effect, including antimicrobial compounds, nutrient competition, and pH changes. In Drosophila melanogaster , Lactiplantibacillus plantarum pathogens. However, the strains of L. studied were derived from laboratory flies or non-fly environments have found be unstable colonizers fly gut...
Animals selectively acquire specific symbiotic gut bacteria from their environments that aid host fitness. To colonize, a symbiont must locate its niche and sustain growth within the gut. Adhesins are bacterial cell surface proteins facilitate attachment to tissues often virulence factors for opportunistic pathogens. However, attachments transient nonspecific, additional mechanisms required infection. In this work, we use live imaging of individual cells colonizing living Drosophila...
Abstract The optimisation of synthetic and natural microbial communities has vast potential for emerging applications in medicine, agriculture industry. Realising this goal is contingent on a close correlation between theory, experiments, the real world. Although temporal pattern resource supply can play major role community assembly, dynamics are commonly treated inconsistently theoretical experimental research. Here we explore how composition varies under continuous supply, typical...
Abstract Animals throughout the metazoa selectively acquire specific symbiotic gut bacteria from their environment that aid host fitness. Current models of colonization suggest these use weakly receptors to stick tissues and results when they in a region overlaps with nutritional niche. An alternative model is unique receptor-ligand binding interactions provide specificity for target niches. Here we live imaging individual bacterial cells colonizing living Drosophila melanogaster show...
Cell- and tissue-level processes often occur across days or weeks, but few imaging methods can capture such long timescales. Here, we describe Bellymount, a simple, noninvasive method for longitudinal of the Drosophila abdomen at subcellular resolution. Bellymounted animals remain live intact, so same individual be imaged serially to yield vivid time series multiday processes. This feature opens door studies internal organs in their native context. Exploiting Bellymount's capabilities, track...
Climate change produces extremes in both temperature and precipitation causing increased drought severity reliance on groundwater resources. Agricultural practices, which rely groundwater, are sensitive to but also sources of contaminants, including nitrate. How agricultural contamination drives geochemistry through microbial metabolism is poorly understood.On an active cow dairy the Central Valley California, we sampled from three wells at depths 4.3 m (two wells) 100 (one well) below...