A5051212192- Genetic and Kidney Cyst Diseases
- Protist diversity and phylogeny
- Microtubule and mitosis dynamics
- Advanced Battery Technologies Research
- Advancements in Battery Materials
- Advanced Battery Materials and Technologies
- Micro and Nano Robotics
- Renal and related cancers
- Analytical Chemistry and Sensors
- Catalysis and Oxidation Reactions
- Coordination Chemistry and Organometallics
- Bacteriophages and microbial interactions
- Synthesis and Catalytic Reactions
- Inorganic and Organometallic Chemistry
- Supercapacitor Materials and Fabrication
- Fluorine in Organic Chemistry
- Synthesis and pharmacology of benzodiazepine derivatives
- Tissue Engineering and Regenerative Medicine
- Autonomous Vehicle Technology and Safety
- Advanced Chemical Sensor Technologies
- Time Series Analysis and Forecasting
- Prenatal Screening and Diagnostics
- Chemical Synthesis and Characterization
- Silk-based biomaterials and applications
- Silkworms and Sericulture Research
University of California, San Francisco
2013-2023
Kyoto University
2001-2022
New York University
2017
Industrial Technology Institute
2011-2016
Nagaoka University of Technology
2011-2015
Tottori University
2014
Central Japan Railway (Japan)
2012
Nagaoka University
2012
Keio University
2012
Nagoya University
2011
In addition to the popular method of fluorescent protein fusion, live cell imaging has now seen more and application epitope tags. The small size these tags may reduce functional perturbation enable signal amplification. To address their background issue, we adapt self-complementing split proteins as for labelling. two tags, GFP11 sfCherry11 are derived from eleventh β-strand super-folder GFP sfCherry, respectively. FP11-tags enables a cost-effective scalable way insert them into endogenous...
The maintenance of flagellar length is believed to require both anterograde and retrograde intraflagellar transport (IFT). However, it difficult uncouple the functions from anterograde, as null mutants in dynein heavy chain 1b (DHC1b) have stumpy flagella, demonstrating solely that IFT required for assembly. We isolated a Chlamydomonas reinhardtii mutant (dhc1b-3) with temperature-sensitive defect DHC1b, enabling inducible inhibition full-length flagella. Although dhc1b-3 flagella at...
The axoneme—the conserved core of eukaryotic cilia and flagella—contains highly specialized doublet microtubules (DMTs). A long-standing question is what protein(s) compose the junctions between two tubules in DMT. Here we identify a flagellar-associated protein (FAP), FAP20, as an inner junction (IJ) component. flagella Chlamydomonas FAP20 mutants have normal length but beat with abnormal symmetrical three-dimensional pattern. In addition, mutant axonemes are liable to disintegrate during...
Ciliogenesis is regulated by context-dependent cellular cues, including some transduced through appendage-like structures on ciliary basal bodies called transition fibers and feet. However, the molecular basis for this regulation not fully understood. The Odf2 gene product, ODF2/cenexin, essential both ciliogenesis formation of distal subdistal appendages centrioles, which become bodies. We examined effects deletion constructs in Odf2-knockout F9 cells. Electron microscopy revealed that...
Abstract Outer dense fibre 2 (Odf2 or ODF2) is a cytoskeletal protein required for flagella (tail)-beating and stability to transport sperm cells from testes the eggs. There are infertile males, including human patients, who have high percentage of decapitated decaudated spermatozoa (DDS), whose semen contains abnormal with tailless heads headless tails due head-neck separation. DDS untreatable in reproductive medicine. We report first time new type Odf2-DDS heterozygous mutant Odf2 +/−...
Organoids, which are self-organizing three-dimensional cultures, provide models that replicate specific cellular components of native tissues or facets organ complexity. We describe a simple method to generate organoid cultures using isolated human tracheobronchial epithelial cells grown in mixed matrix and supplemented at day 14 with the Wnt pathway agonist R-spondin 2 (RSPO2) bone morphogenic protein antagonist Noggin. In contrast previous reports, our produces differentiated...
This paper describes the development of a self-coaching system to improve driving behavior by allowing drivers review record their own activity. By employing stochastic driver-behavior modeling, proposed is able detect wide range potentially hazardous situations, which conventional event data recorders are not capture, including those involving latent risks, themselves unaware. utilizing these automatically detected our web-based offers user-friendly interface for navigate and each situation...
Cilia and flagella are microtubule-based organelles that protrude from the surface of most cells, important to sensing extracellular signals, make a driving force for fluid flow. Maintenance flagellar length requires an active transport process known as intraflagellar (IFT). Recent studies reveal amount IFT injection negatively correlates with flagella. These observations suggest length-dependent feedback regulates IFT. However, it is unknown how cells recognize control Several theoretical...
Eukaryotic cilia and flagella are microtubule-based organelles whose relatively simple shape makes them ideal for investigating the fundamental question of organelle size regulation. Most flagellar materials transported from cell body via an active transport process called intraflagellar (IFT). The rate IFT entry into flagella, known as injection, has been shown to negatively correlate with length. However, it remains unknown how measures length its controls injection. One most-discussed...
New CaO‐P 2 P 5 glass ceramics were prepared by crystallizing rods or plates unidirectionally in a temperature‐gradient furnace. The crystallized showed extremely high bending strength (∼500 to 600 MN/m ) even when subjected severe abrasion treatment with Sic paper. Scanning electron microscopy of fracture surjaces revealed typical fiber‐reinforced‐composite structure. Crystalline phases and crystallographic directions checked X‐ray diflraction. mechanism is discussed terms microstructure.
Cilia are motile and sensory organelles with critical roles in physiology. Ciliary defects can cause numerous human disease symptoms including polycystic kidneys, hydrocephalus, retinal degeneration. Despite the importance of these organelles, their assembly function is not fully understood. The unicellular green alga Chlamydomonas reinhardtii has many advantages as a model system for studies ciliary function. Here we describe our initial efforts to build chemical-biology toolkit augment...