A5055669931- Insect symbiosis and bacterial influences
- Insect Utilization and Effects
- Evolution and Genetic Dynamics
- Forensic Entomology and Diptera Studies
- Gut microbiota and health
- Invertebrate Immune Response Mechanisms
- Vector-Borne Animal Diseases
- Plant and animal studies
- Evolutionary Game Theory and Cooperation
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Immune responses and vaccinations
- Insect behavior and control techniques
- Pharmaceutical and Antibiotic Environmental Impacts
Carnegie Institution for Science
2020-2024
University of Baltimore
2024
Department of Embryology
2020-2023
Carnegie Observatories
2020
University of California, Berkeley
2018
Abstract The gut is continuously invaded by diverse bacteria from the diet and environment, yet microbiome composition relatively stable over time for host species ranging mammals to insects, suggesting host-specific factors may selectively maintain key of bacteria. To investigate specificity, we used gnotobiotic Drosophila , microbial pulse-chase protocols, microscopy stability different strains in fly gut. We show that a host-constructed physical niche foregut binds with strain-level...
Predicting antibiotic efficacy within microbial communities remains highly challenging. Interspecies interactions can impact activity through many mechanisms, including alterations to bacterial physiology. Here, we studied synthetic constructed from the core members of fruit fly gut microbiota. Co-culturing Lactobacillus plantarum with Acetobacter species altered its tolerance transcriptional inhibitor rifampin. By measuring key metabolites and environmental pH, determined that counter...
Animals selectively acquire specific symbiotic gut bacteria from their environments that aid host fitness. To colonize, a symbiont must locate its niche and sustain growth within the gut. Adhesins are bacterial cell surface proteins facilitate attachment to tissues often virulence factors for opportunistic pathogens. However, attachments transient nonspecific, additional mechanisms required infection. In this work, we use live imaging of individual cells colonizing living Drosophila...
Abstract Animals throughout the metazoa selectively acquire specific symbiotic gut bacteria from their environment that aid host fitness. Current models of colonization suggest these use weakly receptors to stick tissues and results when they in a region overlaps with nutritional niche. An alternative model is unique receptor-ligand binding interactions provide specificity for target niches. Here we live imaging individual bacterial cells colonizing living Drosophila melanogaster show...
Abstract The intestines of animals are typically colonized by a complex, relatively stable microbiota that influences health and fitness, but the underlying mechanisms colonization remain poorly understood. As typical animal, fruit fly, Drosophila melanogaster, is associated with consistent set commensal bacterial species, yet reason for this consistency unknown. Here, we use gnotobiotic flies, microscopy, microbial pulse-chase protocols to show niche exists within proventriculus region...
The intestines of animals are colonized by commensal microbes, which impact host development, health, and behavior. Precise quantification colonization is essential for studying the complex interactions between microbe both to validate microbial composition study its effects. Drosophila melanogaster, has a low native diversity economical rear with defined composition, emerged as model organism gut microbiome. Analyzing microbiome an individual requires identification species present their...
The intestines of animals are colonized by commensal microbes, which impact host development, health, and behavior. Precise quantification colonization is essential for studying the complex interactions between microbe both to validate microbial composition study its effects. Drosophila melanogaster, has a low native diversity economical rear with defined composition, emerged as model organism gut microbiome. Analyzing microbiome an individual requires identification species present their...
Infection with Mycobacterium tuberculosis (Mtb) is characterized by an inflammatory response resulting in the formation of granulomas. These tight aggregates immune cells play important role bacterial containment and eventual outcome infection. Monocytes are a major component early to Mtb contribute cellular matrix newly forming granuloma. Therefore, defining which monocyte subset target mycobacterial infection critical. Here, we describe flow-cytometry-based assay analyze infectivity vitro...