A5050503972- Reproductive tract infections research
- Urinary Tract Infections Management
- Clostridium difficile and Clostridium perfringens research
- Bacteriophages and microbial interactions
- Yersinia bacterium, plague, ectoparasites research
- Nuclear Structure and Function
- Urinary and Genital Oncology Studies
- Microbial infections and disease research
- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Herpesvirus Infections and Treatments
- Oral microbiology and periodontitis research
- Advanced Electron Microscopy Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- Advanced Materials Characterization Techniques
- Parvovirus B19 Infection Studies
- Respiratory viral infections research
- HIV/AIDS drug development and treatment
- RNA and protein synthesis mechanisms
- Cervical Cancer and HPV Research
- Dental Anxiety and Anesthesia Techniques
- Microbial Metabolic Engineering and Bioproduction
- HIV Research and Treatment
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
Frederick National Laboratory for Cancer Research
2024-2025
Leidos (United States)
2025
Carnegie Institution for Science
2023-2024
Department of Embryology
2023
University of Maryland, Baltimore
2014-2023
Martin University
2023
University of Maryland Marlene and Stewart Greenebaum Comprehensive Cancer Center
2022
University of California, San Francisco
2001
University of London
1997-2000
London School of Hygiene & Tropical Medicine
2000
Four genes of Chlamydia psittaci strain guinea pig inclusion conjunctivitis (GPIC), whose predicted products are highly homologous to structural and regulatory components a contact‐dependent or type III secretion apparatus, were isolated. Related present in several animal plant bacterial pathogens, these may represent section previously undetected chromosomal virulence locus analogous recently described virulence‐associated loci. The existence strongly suggests that bacteria use pathogenic...
Abstract Secondary caries remains the main reason for dental restoration failure. Replacement of failed restorations accounts 50%–70% all performed. Antibacterial adhesives could inhibit biofilm acids at tooth‐restoration margins, and calcium phosphate (CaP) ions remineralize tooth lesions. The objectives this study were to: (1) incorporate nanoparticles silver (NAg), quaternary ammonium dimethacrylate (QADM), amorphous (NACP) into bonding agent; (2) investigate their effects on dentin...
Abstract The Nannochloropsis genus contains oleaginous microalgae that have served as model systems for developing renewable biodiesel. Recent genomic and transcriptomic studies on species provided insights into the regulation of lipid production in response to nitrogen stress. Previous focused responses short-term stress, but effect long-term deprivation remains largely unknown. In this study, physiological proteomic approaches were combined understand mechanisms by which oceanica IMET1 is...
Investigations conducted on feral African Sacred Ibises (Threskiornis aethiopicus) in western France led to the isolation of a strain with chlamydial genetic determinants. Ultrastructural analysis, comparative sequence analysis 16S rRNA gene, ompA, and concatenate 31 highly conserved genes, as well determination whole genome confirmed relatedness new isolate members Chlamydiaceae, while, at same time demonstrating unique position outside currently recognized species this family. We propose...
Abstract Background Immune-checkpoint inhibitors (ICIs) changed the therapeutic landscape of patients with lung cancer. However, only a subset them derived clinical benefit and evidenced need to identify reliable predictive biomarkers. Liquid biopsy is non-invasive repeatable analysis biological material in body fluids promising tool for cancer biomarkers discovery. In particular, there growing evidence that extracellular vesicles (EVs) play an important role tumor progression tumor-immune...
Lenacapavir (GS-6207; LEN) is a potent HIV-1 capsid inhibitor approved for treating multidrug-resistant infection. LEN binds to hydrophobic pocket between neighboring (CA) proteins in hexamers and stabilizes the lattice, but its effect on capsids not fully understood. Here, we labeled with green fluorescent protein fused CA (GFP-CA) or fluid-phase GFP content marker (cmGFP) assess LEN’s impact capsids. cores GFP-CA, cmGFP, could be immunostained an anti-GFP antibody were less sensitive...
Infections caused by the obligate intracellular pathogen Chlamydia trachomatis have a marked impact on human health. C. serovariants are leading cause of bacterial sexually transmitted disease and infectious preventable blindness. Despite decades effort, there is no practical vaccine against diseases. Here we report that all reference serotypes responsible for blinding trachoma synthesize highly conserved surface-exposed antigen termed polymorphic membrane protein D (PmpD). We show Ab...
Although endosomal compartments have been suggested to play a role in unconventional protein secretion, there is scarce experimental evidence for such involvement. Here we report that recycling endosomes are essential externalization of cytoplasmic secretory tissue transglutaminase (tTG). The de novo synthesized tTG does not follow the classical ER/Golgi-dependent secretion pathway, but targeted perinuclear endosomes, and delivered inside these vesicles prior externalization. On its route...
Many intracellular pathogens subvert host membrane trafficking pathways to promote their replication. Toxoplasma multiplies in a membrane-bound parasitophorous vacuole (PV) that interacts with mammalian organelles and intercepts Golgi Rab vesicles acquire sphingolipids. The mechanisms of vesicle internalization processing within the PV remain undefined. We demonstrate sequesters broad range into PV. Correlative light electron microscopy analysis infected cells illustrates intravacuolar Rab1A...
Genomic analysis of the Chlamydiaceae has revealed a multigene family encoding large, putatively autotransported polymorphic membrane proteins (Pmps) with nine members in sexually transmitted pathogen Chlamydia trachomatis. While various pathogenesis-related functions are emerging for Pmps, observed genotypic and phenotypic variation among several chlamydial Pmps species led us to hypothesize that pmp gene repertoire is basis previously undetected mechanism antigenic variation. To test this...
SINC, a new type III secreted protein of the avian and human pathogen Chlamydia psittaci, uniquely targets nuclear envelope C. psittaci-infected cells uninfected neighboring cells. Digitonin-permeabilization studies SINC-GFP-transfected HeLa indicate that SINC inner membrane. localization at was blocked by importazole, confirming import into nucleus. Candidate partners were identified proximity to biotin ligase-fused in HEK293 mass spectrometry (BioID). This strategy 22 candidates with high...
Animals selectively acquire specific symbiotic gut bacteria from their environments that aid host fitness. To colonize, a symbiont must locate its niche and sustain growth within the gut. Adhesins are bacterial cell surface proteins facilitate attachment to tissues often virulence factors for opportunistic pathogens. However, attachments transient nonspecific, additional mechanisms required infection. In this work, we use live imaging of individual cells colonizing living Drosophila...
The hypothesized variable expression of polymorphic membrane proteins (PmpA-PmpI) in Chlamydia trachomatis-infected patients was tested by examination the each Pmp subtype vitro-grown C. trachomatis. A panel monospecific polyclonal and monoclonal antibodies used to demonstrate surface exposure Pmps differential immunofluorescence (IF) with without prior detergent permeabilization paraformaldehyde-fixed inclusions for selected immunogold labelling. Although specific transcript detected pmp...
The proinflammatory state associated with diabetes mellitus (DM) remains poorly understood. We found patients DM have 3- to 14-fold elevations of blood-borne microparticles (MPs) that bind phalloidin (Ph; Ph positive [+] MPs), indicating the presence F-actin on their surface. hypothesized F-actin-coated MPs were an unrecognized cause for DM-associated status. Ph+MPs, but not Ph-negative MPs, activate human and murine (Mus musculus) neutrophils through biophysical attributes membrane...
The chlamydial life cycle involves the intimate interaction of components infectious elementary body (EB) surface with receptors on susceptible eukaryotic cell plasma membrane. We have developed an in vitro ligand binding assay system for identification and characterization detergent-extracted EB envelope proteins capable to glutaraldehyde-fixed HeLa surfaces. With this assay, developmentally regulated cysteine-rich protein Omp2 Chlamydia psittaci strain guinea pig inclusion conjunctivitis...
Abstract Background Like all retroviruses, two kinds of viral DNA are present in the nucleus HIV-infected cells: integrated and a pool unintegrated containing linear circular forms. For most part, it has been difficult to examine role forms life cycle presence form, or distinguish respective contributions context DNA. Results In our approach, we constructed 1-LTR form HIV order study its expression isolation from other forms; derived genomic lacking 5’-LTR (1-LTR ) molecular clone HIV. This...
Journal Article Workshop on In Vitro Neutralization of Chlamydia trachomatis: Summary Proceedings Get access G. I. Byrne, Byrne Reprints or correspondence: Dr. Gerald Dept. Medical Microbiology, University Wisconsin, 1300 Ave., Madison, WI 53706. Search for other works by this author on: Oxford Academic PubMed Google Scholar R. S. Stephens, Stephens Ada, Ada H. D. Caldwell, Caldwell Su, Su P. Morrison, Morrison B. Van Der Pol, Pol Bavoil, Bavoil L. Bobo, Bobo Everson, Everson ... Show more...
Late Chlamydia trachomatis inclusions express each member of the surface-exposed polymorphic membrane protein family (Pmp subtypes A through I) with a reproducible distribution fully-on, fully-off and intermediate phenotypes. This observation is consistent observed variable Pmp antibody profiles in C. trachomatis-infected patients has led to hypothesis that pmp gene forms basis phase variation-like mechanism antigenic variation. Here we investigate compare developmental expression nine genes...