A5076075079- Adipokines, Inflammation, and Metabolic Diseases
- Diabetic Foot Ulcer Assessment and Management
- Extracellular vesicles in disease
- Traumatic Brain Injury and Neurovascular Disturbances
- Autophagy in Disease and Therapy
- Cardiovascular and Diving-Related Complications
- Wound Healing and Treatments
- Advanced Glycation End Products research
- Diabetes and associated disorders
- Signaling Pathways in Disease
- Intracerebral and Subarachnoid Hemorrhage Research
- Heme Oxygenase-1 and Carbon Monoxide
- Calcium signaling and nucleotide metabolism
- Cerebrospinal fluid and hydrocephalus
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Galectins and Cancer Biology
- Natural Antidiabetic Agents Studies
- Liver Disease Diagnosis and Treatment
- Protein Tyrosine Phosphatases
- Phytochemistry and Bioactivity Studies
- Gastrointestinal disorders and treatments
- Histone Deacetylase Inhibitors Research
- Body Contouring and Surgery
- COVID-19 Clinical Research Studies
- Neuroscience of respiration and sleep
University of Maryland, Baltimore
2018-2025
University of Maryland Medical Center
2024
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2023
National Institutes of Health
2023
Ben-Gurion University of the Negev
2014-2022
City Of Hope National Medical Center
2022
City of Hope
2022
National Eye Institute
2022
Banaras Hindu University
2011-2015
Institute of Medical Sciences
2011-2015
Blood-borne extracellular vesicles and inflammatory mediators were evaluated in divers using a closed circuit rebreathing apparatus custom-mixed gases to diminish some diving risks. “Deep” (n = 8) dove once mean (±SD) 102.5 ± 1.2 m of sea water (msw) for 167.3 11.5 min. “Shallow” 6) 3 times on day 1, then repetitively over 7 days 16.4 3.7 msw, 49.9 11.9 There statistically significant elevations microparticles (MPs) deep (day 1) shallow at that expressed proteins specific microglia,...
Oxygen is a powerful trigger for cellular reactions, but there are few comparative investigations assessing the effects over large range of partial pressures. We investigated metabolic response to single exposures either normobaric (10%, 15%, 30%, 100%) or hyperbaric (1.4 ATA, 2.5 ATA) oxygen. Forty-eight healthy subjects (32 males/16 females; age: 43.7 ± 13.4 years, height: 172.7 10.07 cm; weight 68.4 15.7 kg) were randomly assigned, and blood samples taken before 2 h after each exposure....
The proinflammatory state associated with diabetes mellitus (DM) remains poorly understood. We found patients DM have 3- to 14-fold elevations of blood-borne microparticles (MPs) that bind phalloidin (Ph; Ph positive [+] MPs), indicating the presence F-actin on their surface. hypothesized F-actin-coated MPs were an unrecognized cause for DM-associated status. Ph+MPs, but not Ph-negative MPs, activate human and murine (Mus musculus) neutrophils through biophysical attributes membrane...
Uncontrolled blood sugar is a major cause of vascular complications and delayed wound healing in diabetes mellitus. During process, normally, apoptosis responsible for events such as removal inflammatory cells evolution granulation tissue into scar which occur during the late phase healing. Early can lead to abnormal by removing including fibroblast, endothelial cell, small vessels. To determine role association with hyperglycemia diabetic healing, apoptosis-related intracellular marker...
The mechanism for neurological deficits from carbon monoxide (CO) poisoning is unclear. In a series of 150 patients with CO poisoning, we found marked elevations blood-borne inflammatory filamentous (F-) actin-coated microparticles (MPs), neutrophil activation, and 90% reduction in the normal level plasma gelsolin (pGSN), protein capable lysing F-actin-coated MPs. This led to studies murine model where same events occur cause neuroinflammation cognitive dysfunction. All are recapitulated...
A murine model of central nervous system (CNS) decompression sickness demonstrates that high gas pressure activates astrocytes and microglia triggering inflammatory microparticle (MP) production. Thrombospondin-expressing MPs are released from the CNS via enhanced glymphatic flow to systemic circulation where they activate neutrophils. Secondary production neutrophil-derived causes further cell activation neutrophil adherence brain microvasculature establishing a feed-forward neuroinflammatory cycle.
The T cell lineage–restricted protein THEMIS plays a critical role in development at the positive selection stage. In SHP1 activation model, is proposed to enhance activity of tyrosine phosphatase (encoded by Ptpn6 ), thereby dampening antigen receptor (TCR) signaling and preventing inappropriate negative CD4 + CD8 thymocytes positively selecting ligands. contrast, inhibition suppress activity, rendering more sensitive TCR initiated low-affinity ligands promote selection. We sought resolve...
A blood component analysis is an early step for evaluating inflammatory disorders, but it can be unfeasible in some settings. This pilot study assessed whether extracellular vesicle (EV) changes perspiration are parallel to those occurring as alternative or complementary option diagnose response. In studies, EVs were analyzed and obtained before after five self-contained underwater breathing apparatus (SCUBA) divers at the National Aquarium Baltimore performed a dive 3.98 m of sea water 40...
The T-lineage restricted protein THEMIS has been shown to play a critical role in T cell development. THEMIS, via its distinctive CABIT domains, inhibits the catalytic activity of tyrosine phosphatase SHP1 (PTPN6). and bind ubiquitous cytosolic adapter GRB2, purported formation tri-molecular THEMIS-GRB2-SHP1 complex facilitates inactivation by THEMIS. importance this function GRB2 among numerous documented activities is unclear as binds multiple proteins participates several signaling...
Elevated glymphatic flow due to astrocyte and microglial activation from high-pressure exposure triggers release of microparticles (MPs) the circulation where neutrophil production filamentous (F)-actin expressing MPs result in a persistent feed-forward neuroinflammatory cycle functional deficits lasting for at least 12 days.
Diabetes mellitus is a metabolic disorder. It the most common cause of blindness, kidney failure, and amputations in adults leading heart disease stroke. These patients are more prone to infection which shows that there suppressed cell mediated immunity during diabetes progression. Lymphocytes play crucial role maintenance cellular & humoral both its development requires selection remove potentially auto reactive cells via apoptosis. Apoptosis lymphocyte clones pivotal purging body...
Background: Lymphocytes play an important role in wound healing and the removal of circulating T lymphocytes inhibits cascade. Decreased stimulation survival factors increased levels dead signals may lead to malfunction many cells, including lymphocytes. Aims & Objective: To explore involvement healing. Material Methods: Study participants were divided into three groups: group A, B C (30 each). Annexin-V-FITC+CD-3-PE kit used for lymphocyte apoptosis estimation diabetic foot patients by...
Abstract Introduction: Diabetes mellitus (DM) is a group of metabolic disorders which opens the door for many diseases leading to various complications like neuropathy, nephropathy and retinopathy. Type 2 diabetes (T2DM) associated with elevated level oxidative stress, one most important factors responsible development chronic this disease. Antioxidants reduced glutathione (GSH), superoxide dismutase (SOD) catalase protects cells against damage. Purpose: Our present study suggests that...