Trichomoniasis vaginalis is the most common nonviral sexually transmitted infection (STI) worldwide, with a particularly high prevalence in regions of human immunodeficiency virus (HIV) endemicity. However, its impact as cofactor for HIV acquisition poorly understood.Samples from 213 women who experienced seroconversion (cases) during longitudinal study involving 4450 Uganda and Zimbabwe were matched samples HIV-uninfected (controls). All underwent polymerase chain reaction (PCR) analysis...

Background The microbiome of the male urogenital tract is poorly described but it has been suggested that bacterial colonization urethra might impact risk sexually transmitted infection (STI). Previous cultivation-dependent studies showed a variety non-pathogenic bacteria colonize did not thoroughly characterize these microbiomes or establish links between compositions urethral and STI. Methodology/Findings Here, we used 16S rRNA PCR sequencing to identify in urine specimens collected from...

Repeated Chlamydia trachomatis infections are common among young sexually active women. The relative frequency of reinfection and antibiotic treatment failure is undefined.Adolescent women enrolled in a longitudinal cohort had behavioral transmitted infection assessments performed every 3 months, including amplification tests for C. trachomatis, ompA genotyping, interviews diary entries to document sex partner-specific coitus event-specific condom use. were classified as or by use an...

Tests for Chlamydia trachomatis and Neisseria gonorrhoeae, which can provide results rapidly to guide therapeutic decision-making, offer patient care advantages over laboratory-based tests that require several days results. We compared from the Cepheid GeneXpert CT/NG (Xpert) assay two currently approved nucleic acid amplification assays in 1,722 female 1,387 male volunteers. Results chlamydia females demonstrated sensitivities endocervical, vaginal, urine samples of 97.4%, 98.7%, 97.6%,...

Lactobacillus-dominated vaginal microbiotas are associated with reproductive health and STI resistance in women, whereas altered bacterial vaginosis (BV), risk poor outcomes. Putative taxa have been observed male first-catch urine, urethral swab coronal sulcus (CS) specimens but the significance of these observations is unclear. We used 16 S rRNA sequencing to characterize microbiota CS urine collected from 18 adolescent men over three consecutive months. most participants were more stable...

ABSTRACT The performance of the Becton Dickinson BDProbe Tec ET System Chlamydia trachomatis and Neisseria gonorrhoeae Amplified DNA Assays (BD Biosciences, Sparks, Md.) was evaluated in a multicenter study. Specimens were collected from 2,109 men women, with or without symptoms, attending sexually transmitted disease, family planning, obstetrics gynecology clinics. Both swab urine samples collected, results obtained 4,131 specimens compared to those culture LCx nucleic acid amplification...

Urine is the CDC-recommended specimen for STI testing. It was unknown if bacterial communities (microbiomes) in urine reflected those distal male urethra. We compared microbiomes of 32 paired and urethral swab specimens obtained from adult men attending an STD clinic, by 16S rRNA PCR deep pyrosequencing. Microbiomes swabs were remarkably similar, regardless status subjects. Thus, can be used to characterize when are undesirable, such as population-based studies microbiome or where multiple...

ABSTRACT Puberty is an important developmental stage wherein hormonal shifts mediate the physical and physiological changes that lead to menarche, but until now, bacterial composition of vaginal microbiota during this period has been poorly characterized. We performed a prospective longitudinal study perimenarcheal girls gain insight into timing sequence occur in vulvar puberty. The enrolled 31 healthy, premenarcheal between ages 10 12 years collected swabs quarterly for up 3 years....

In vitro susceptibility testing was done on urogenital isolates of Chlamydia trachomatis from five patients, four whom were suspected treatment failures. At least one isolate each patient wasresistant to tetracycline at concentrations ⩾8 p.g/ml, although <1% a population organisms showed high-level resistance. Fully resistant populations selected bypassage through 8 p.glml either died or lost their resistance further passage in antibiotic-freemedium. Relatively large inocula required...

In Brief Background: Trichomonas vaginalis (T. vaginalis) is the most common nonviral sexually transmitted infection in world. Despite coexisting global epidemics of T. and HIV, little attention has focused on emerging evidence that increases susceptibility to, potentially transmission of, HIV. Methods: We evaluated context a multisite, randomized controlled trial amongst women South Africa Zimbabwe, to determine first, if risk HIV acquisition was increased among recently infected with...

Objective. The purpose of this study was to determine the prevalence chronic ankle instability among high school and collegiate athletes. Design. Descriptive epidemiological survey. Methods. Athletes from four schools a division I university were contacted participate. For athletes, questionnaire packet distributed during preparticipation physicals. parental consent obtained then questionnaires physicals, parent meetings, or individual team meetings. All athletes completed Cumberland Ankle...

IntroductionThis study evaluates the impact of COVID-19 pandemic on testing for common sexually transmitted infections. Specifically, changes are measured in chlamydia and gonorrhea case detection among patients aged 14–49 years during pandemic.MethodsU.S. positivity were analyzed basis >18.6 million tests (13.6 female 4.7 male patients) performed by a national reference clinical laboratory from January 2019 through June 2020.ResultsChlamydia reached nadir early April 2020, with decreases...

To guide future preexposure prophylaxis (PrEP) implementation for women who inject drugs (WWID), a population increasingly represented in new HIV cases the United States, we present results from demonstration project integrated within syringe services program (SSP) Philadelphia, PA.WWID ≥18 years were educated about and offered 24 weeks of daily PrEP. Participants completed surveys clinical assessments at baseline 1, 3, 12, 24. We used descriptive statistics to estimate...

ABSTRACT Because self-collected vaginal swabs (VS) are potentially very useful for screening asymptomatic women Chlamydia trachomatis infection, a multicenter study evaluated that specimen with nucleic acid amplification tests (NAATs). The objective was to determine whether VS equal Food and Drug Administration (FDA)-cleared specimens (cervical first-catch urines [FCU]) diagnosing genital chlamydial infection. All NAATs then commercially available (October 1996 October 1999) were used...

BackgroundTrichomonas vaginalis infection is a sexually transmitted (STI) linked with reproductive health complications. However, few data exist concerning the epidemiologic profile of this pathogen in adolescent women, group at high risk for other STIs

ABSTRACT The fully automated COBAS AMPLICOR CT/NG and semiautomated tests were evaluated in a multicenter trial for their ability to detect Neisseria gonorrhoeae infections. Test performance compared that of culturing was 2,192 matched endocervical swab urine specimens obtained from women 1,981 urethral men. Culture-negative, PCR-positive tested positive confirmatory PCR test an alternative target sequence within the N. 16S rRNA gene considered be true positives. overall prevalences...

ABSTRACT The fully automated COBAS AMPLICOR CT/NG and semiautomated tests were evaluated in a multicenter trial for the ability to detect Chlamydia trachomatis infections. Test performance compared that of culture was 2,236 matched endocervical swab urine specimens obtained from women 1,940 urethral men. Culture-negative, PCR-positive tested positive direct fluorescent-antibody test or confirmatory PCR an alternative target sequence resolved as true positives. overall prevalences chlamydia...

Objective: To evaluate interrelationships between bacterial vaginosis (BV), vaginal yeast, practices (cleansing and drying/tightening), mucosal inflammation, HIV acquisition. Methods: A multicenter, prospective, observational cohort study was conducted, enrolling 4531 HIV-negative women aged 18 to 35 years attending family planning clinics in Zimbabwe Uganda. Participants were tested for reproductive tract infections interviewed about every 3 months 15 24 months. BV measured by Gram stain...