A5078151077- Reproductive tract infections research
- Cervical Cancer and HPV Research
- Urinary Tract Infections Management
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Bacterial Genetics and Biotechnology
- Genital Health and Disease
- Pancreatic and Hepatic Oncology Research
- Syphilis Diagnosis and Treatment
- Urological Disorders and Treatments
- Adolescent Sexual and Reproductive Health
- CRISPR and Genetic Engineering
- Renal cell carcinoma treatment
- Influenza Virus Research Studies
- Cancer Genomics and Diagnostics
- Animal Disease Management and Epidemiology
- Immunotherapy and Immune Responses
- TGF-β signaling in diseases
- Immune Response and Inflammation
- Bacterial Infections and Vaccines
- Herpesvirus Infections and Treatments
Indiana University School of Medicine
2015-2025
Indiana University – Purdue University Indianapolis
2015-2025
GlaxoSmithKline (United States)
2022-2024
Aldevron (United States)
2023
Nature Technology Corporation (United States)
2009-2022
University of Washington
2016-2021
Seattle University
2021
Pfizer (United States)
2010-2020
Office of Infectious Diseases
2005-2019
Tufts University
2017
The benefits of screening hepatitis B surface antigen (HBsAg)-positive carriers for hepatocellular carcinoma (HCC) in terms long-term survival have not been established. We conducted a prospective 16-year, population-based cohort study to determine the impact HCC 1,487 HBsAg-positive Alaska native with alpha-fetoprotein (AFP) determinations every 6 months. Men and nonpregnant women an elevated AFP level were evaluated presence by ultrasound (US) examination. rate patients whose was detected...
Significance How did the first cells on Earth arise? In a minimal cell, membrane separates proteins and RNA from surrounding aqueous environment. Cell-like membranes spontaneously assemble simple prebiotic surfactants called fatty acids. However, acid are unstable in solutions containing salts that were likely present environments of early Earth. We find amino acids, building blocks proteins, bind to stabilize them against salts. Moreover, enhanced stabilization persists after dilution as...
BackgroundTrichomonas vaginalis infection is a sexually transmitted (STI) linked with reproductive health complications. However, few data exist concerning the epidemiologic profile of this pathogen in adolescent women, group at high risk for other STIs
Protein-mediated membrane fusion is an essential step in many fundamental biological events, including enveloped virus infection. The nature of protein and intermediates the sequence remodeling during these processes remain poorly understood. Here we used cryo-electron tomography (cryo-ET) to image interplay between influenza vesicles with a range lipid compositions. By following population kinetics imaged by cryo-ET, found that commenced hemagglutinin spikes grappling onto target membrane,...
ABSTRACT Increasing levels of plasmid vector-mediated activation innate immune signaling pathways is an approach to improve DNA vaccine-induced adaptive immunity for infectious disease and cancer applications. Retinoic acid-inducible gene I (RIG-I) a critical cytoplasmic double-stranded RNA (dsRNA) pattern receptor required in response viral infection. Activation RIG-I leads type interferon (IFN) inflammatory cytokine production through promoter stimulator 1 (IPS-1)-mediated regulatory...
Trichomonas vaginalis is a sexually transmitted infection, which largely underestimated because of ineffective screening protocols and lack public health attention.Two studies were conducted to assess the frequency missed diagnosis T. when using current routine practices for in high-risk female populations. The first study compares rate positivity detected wet preparation microscopy number cases found polymerase chain reaction (PCR) residual samples from women attending disease clinic....
Trichomonas vaginalis is the most prevalent nonviral sexually transmitted infection worldwide, and improved diagnostic methods are critical for controlling this pathogen. Diagnostic assays that can be used in conjunction with routine chlamydia/gonorrhea nucleic acid-based screening likely to have impact on disease control. Here we describe performance of new BD T. Qx (TVQ) amplified DNA assay, which performed automated Viper system. We focus data from vaginal swab samples, since specimen...
<h3>Background</h3> There is little information regarding the composition of peripheral blood immunity in sarcoma patients and even less context pediatric sarcomas. We describe immune status using flow cytometry with osteosarcoma Ewing demonstrate excessive CD14 tumor tissues. <h3>Methods</h3> Peripheral from OS ES was collected at diagnosis or relapse, used for phenotyping 74 different leukocyte phenotypes. Blood young adult healthy volunteers as controls. Tumor tissues were analyzed by...
The BD Max CT/GC/TV (MAX) assay is a true multiplex for simultaneous detection of chlamydia (CT), gonorrhea (GC), and trichomonas (TV). We evaluated performance women using endocervical vaginal swabs as well urine specimens. A total 1,143 were tested CT, GC, TV and, subsequently, another 847 (1,990 women) CT GC only, with positivity rates 7.1%, 2.3%, 13.5%, respectively. In men, the was determined only not assessed in male samples. Among 181/830 (21.8%) 108/840 (12.9%) infections,...
The origin, composition, and significance of the distal male urethral microbiome are unclear, but vaginal dysbiosis is linked to new sex partners several urogynecological syndromes. We characterized 110 specimens from men without symptoms, infections, or inflammation using shotgun metagenomics. Most contain characteristic lactic acid bacteria Corynebacterium spp. In contrast, associated with were present only in who reported intercourse. Sexual behavior, not other evaluated behavioral,...
This study aims to determine the relative contribution of oocyte and/or sperm dysfunction reduction fertilization rates in vitro cases minor endometriosis and prolonged unexplained infertility. The results in-vitro (IVF) treatment with ovarian stimulation have been compared between couples above conditions women tubal infertility (as control for function) use donor spermatozoa function). Fertilization cleavage using husband's were significantly reduced (56%, n = 194, P < 0.001) further (52%,...
Background. Leflunomide is an experimental drug with demonstrated ability to prevent and reverse acute allograft xenograft rejection. The two biochemical activities reported for the active metabolite of leflunomide, A77 1726, are inhibition tyrosine phosphorylation dihydroorotate dehydrogenase, enzyme necessary de novo pyrimidine synthesis. These can be distinctly separated in vitro by use uridine, which reverses anti-proliferative effects 1726 caused We report effect uridine on vivo...
ABSTRACT Trichomonas vaginalis PCR using reagents from a commercially available assay for Chlamydia trachomatis and Neisseria gonorrhoeae was evaluated detection of infection in women men attending sexually transmitted disease clinic. Evaluations included three primer sets, endocervical swabs, vaginal swabs urine, various storage conditions. The TVK3/TVK7 set optimal our hands with sensitivities ranging 69.5 to 96.8%. In all comparisons, T. performed better than routine diagnostics...
Bacterial plasmids are the vectors of choice for DNA vaccines and short-term gene therapeutics. Growing plasmid by microbial (Escherichia coli) fermentation is usually combined with alkaline lysis/chromatography methods purification. To date, typical media processes result in yields 100-250 mg DNA/l culture medium, using standard high-copy pUC origin-containing plasmids. In order to address this initial yield-limiting upstream step, we identified novel control parameters fed-batch...
DNA vaccines have tremendous potential for rapid deployment in pandemic applications, wherein a new antigen is "plugged" into validated vector, and rapidly produced validated, fermentation-purification process. For this application, it essential that the vector fermentation process function with variety of different genes. However, many genes are unpredictably "toxic" or otherwise low yielding standard processes. We report cell bank unit operation innovations reduce plasmid-mediated...
An assessment has been made of 25 patients who have undergone ileorectal anastomosis for Crohn's disease. The rate anastomotic leakage was high (32%) but fatal in only one patient. likelihood not affected by a safety valve ileostomy, failure to excise all diseased bowel, or steroid therapy. overall recurrence 60 per cent. This the age patient, duration disease, therapy, leak adversely presence concurrent small bowel disease and residual rectal at site anastomosis.