A5012477988- Reproductive tract infections research
- Gut microbiota and health
- Urinary Tract Infections Management
- Reproductive System and Pregnancy
- Genomics and Phylogenetic Studies
- Clostridium difficile and Clostridium perfringens research
- Antibiotic Resistance in Bacteria
- Plant and fungal interactions
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Microbial infections and disease research
- Yersinia bacterium, plague, ectoparasites research
- Cervical Cancer and HPV Research
- Syphilis Diagnosis and Treatment
- Bacterial Identification and Susceptibility Testing
- Plant Pathogenic Bacteria Studies
- Mycobacterium research and diagnosis
- Reproductive Physiology in Livestock
- Plant Toxicity and Pharmacological Properties
- HIV, TB, and STIs Epidemiology
- Brucella: diagnosis, epidemiology, treatment
- Bacterial Genetics and Biotechnology
- Ruminant Nutrition and Digestive Physiology
- HIV Research and Treatment
- Vibrio bacteria research studies
University of Technology Sydney
2015-2025
American Dental Education Association
2021
John Wiley & Sons (United States)
2021
University of Maryland, Baltimore
2004-2016
University of Maryland, College Park
2014
University of Bologna
2014
Human Genome Sciences (United States)
2008-2013
J. Craig Venter Institute
2006-2009
Los Alamos National Laboratory
2007
Biotechnology Institute
2004
The complete genome sequence of Enterococcus faecalis V583, a vancomycin-resistant clinical isolate, revealed that more than quarter the consists probable mobile or foreign DNA. One predicted elements is previously unknown vanB vancomycin-resistance conjugative transposon. Three plasmids were identified, including two pheromone-sensing plasmids, one encoding undescribed pheromone inhibitor. apparent propensity for incorporation probably contributed to rapid acquisition and dissemination drug...
Whole-genome sequencing has been skewed toward bacterial pathogens as a consequence of the prioritization medical and veterinary diseases. However, it is becoming clear that in order to accurately measure genetic variation within between pathogenic groups, multiple isolates, well commensal species, must be sequenced. This study examined pangenomic content Escherichia coli. Six distinct E. coli pathovars can distinguished using molecular or phenotypic markers, but only two six have subjected...
Pseudomonas fluorescens Pf-5 is a plant commensal bacterium that inhabits the rhizosphere and produces secondary metabolites suppress soilborne pathogens. The complete sequence of 7.1-Mb genome was determined. We analyzed repeat sequences to identify genomic islands that, together with other approaches, suggested P. Pf-5's recent lateral acquisitions include six metabolite gene clusters, seven phage regions mobile island. identified various features contribute its lifestyle on plants,...
Clostridium perfringens is a Gram-positive, anaerobic spore-forming bacterium commonly found in soil, sediments, and the human gastrointestinal tract. C. responsible for wide spectrum of disease, including food poisoning, gas gangrene (clostridial myonecrosis), enteritis necroticans, non-foodborne infections. The complete genome sequences strain ATCC 13124, isolate species type strain, enterotoxin-producing poisoning SM101, were determined compared with published 13 genome. Comparison three...
We present the complete 2,843,201-bp genome sequence of Treponema denticola (ATCC 35405) an oral spirochete associated with periodontal disease. Analysis T. reveals factors mediating coaggregation, cell signaling, stress protection, and other competitive cooperative measures, consistent its pathogenic nature lifestyle within mixed-species environment subgingival dental plaque. Comparisons previously sequenced genomes revealed specific contributing to differences similarities in physiology as...
The first microbial genome sequence, Haemophilus influenzae, was published in 1995. Since then, more than 400 sequences have been completed or commenced. This massive influx of data provides the opportunity to obtain biological insights through comparative genomics. However few tools are available for this scale analysis.The BLAST Score Ratio (BSR) approach, implemented a Perl script, classifies all putative peptides within three genomes using measure similarity based on ratio scores. output...
Coastal aquatic environments are typically more highly productive and dynamic than open ocean ones. Despite these differences, cyanobacteria from the genus Synechococcus important primary producers in both types of ecosystems. We have found that genome a coastal cyanobacterium, sp. strain CC9311, has significant differences an strain, WH8102, consistent with between their respective environments. CC9311 greater capacity to sense respond changes its (coastal) environment. It much larger...
The complete DNA sequence of the aerobic cellulolytic soil bacterium Cytophaga hutchinsonii, which belongs to phylum Bacteroidetes, is presented. genome consists a single, circular, 4.43-Mb chromosome containing 3,790 open reading frames, 1,986 have been assigned tentative function. Two most striking characteristics C. hutchinsonii are its rapid gliding motility over surfaces and contact-dependent digestion crystalline cellulose. mechanism not known, but contains homologs for each gld genes...
Chlamydiae are evolutionarily well-separated bacteria that live exclusively within eukaryotic host cells. They include important human pathogens such as Chlamydia trachomatis well symbionts of protozoa. As these experimentally challenging and genetically intractable, our knowledge about them is still limited. In this study, we obtained the genome sequences Simkania negevensis Z, Waddlia chondrophila 2032/99, Parachlamydia acanthamoebae UV-7. This enabled us to perform first comprehensive...
We know very little about the microbiota inhabiting upper female reproductive tract and how it impacts on fertility.This pilot study aimed to examine vaginal, cervical endometrial for women with a history of infertility compared fertility.Using retrospective case-control design, were recruited collection samples. The composition was analysed by 16S ribosomal RNA (rRNA) gene amplification expression selected human genes quantitative reverse transcription polymerase chain reaction.Sixty-five...
We developed an RNA-Seq-based method to simultaneously capture prokaryotic and eukaryotic expression profiles of cells infected with intracellular bacteria. As proof principle, this was applied Chlamydia trachomatis-infected epithelial cell monolayers in vitro, successfully obtaining transcriptomes both C. trachomatis the host at 1 24 hours post-infection. Chlamydiae are obligate bacterial pathogens that cause a range mammalian diseases. In humans chlamydiae responsible for most common...
Investigations conducted on feral African Sacred Ibises (Threskiornis aethiopicus) in western France led to the isolation of a strain with chlamydial genetic determinants. Ultrastructural analysis, comparative sequence analysis 16S rRNA gene, ompA, and concatenate 31 highly conserved genes, as well determination whole genome confirmed relatedness new isolate members Chlamydiaceae, while, at same time demonstrating unique position outside currently recognized species this family. We propose...
Brucella ovis is a veterinary pathogen associated with epididymitis in sheep. Despite its genetic similarity to the zoonotic pathogens B. abortus, melitensis and suis, does not cause disease. Genomic analysis of type strain ATCC25840 revealed high percentage pseudogenes increased numbers transposable elements compared species, suggesting that genome degradation has occurred concomitant narrowing host range ovis. The absence genomic island 2, encoding functions required for lipopolysaccharide...
Abstract Chlamydia trachomatis is a global cause of blinding trachoma and sexually transmitted infections (STIs). We used comparative genomics the family Chlamydiaceae to select conserved housekeeping genes for C. multilocus sequencing, characterizing 19 reference 68 clinical isolates from 6 continental/subcontinental regions. There were 44 sequence types (ST). Identical STs STI recovered different regions, whereas restricted by continent. Twenty-nine 52 alleles had nonuniform distributions...
Chlamydia psittaci is an avian pathogen and zoonotic agent of atypical pneumonia. The most pathogenic C. strains cluster into the 6BC clade, predicted to have recently emerged globally. Exposure infected parrots a risk factor with limited evidence also indirect exposure risk. Genome sequencing was performed on six Australian human single strain isolated over 9 year period. Only one five patients had explicit psittacine contact. Genomics analyses revealed that are remarkably similar,...
By sequencing a total of 2089 bp the 16S rRNA and phoE genes it was demonstrated that Calymmatobacterium granulomatis (the causative organism donovanosis) shows high level identity with Klebsiella species pathogenic to humans (Klebsiella pneumoniae, rhinoscleromatis). It is proposed C. should be reclassified as comb. nov. An emended description genus given.
Zoonotic infections are a growing threat to global health. Chlamydia pneumoniae is major human pathogen that widespread in populations, causing acute respiratory disease, and has been associated with chronic disease. C. was first identified solely populations; however, its host range now includes other mammals, marsupials, amphibians, reptiles. Australian koalas (Phascolarctos cinereus) widely infected two species of Chlamydia, pecorum pneumoniae. Transmission between animals humans not...
ABSTRACT The mouse chlamydial pathogen Chlamydia muridarum has been used as a model organism for the study of human trachomatis urogenital and respiratory tract infections. To date, two commonly C. isolates have interchangeably are essentially taken to be identical. Herein, we present data that indicate this is not case. Weiss isolate Nigg varied significantly in their virulences vivo possessed different growth characteristics vitro. Distinct differences were observed intravaginal 50%...
ABSTRACT It is not currently possible to predict the probability of whether a woman with chlamydial genital infection will develop pelvic inflammatory disease (PID). To determine if specific biomarkers may be associated distinct pathotypes, we utilized two Chlamydia muridarum variants ( C. Var001 [CmVar001] and CmVar004) that differ in their abilities elicit upper tract pathology mouse model. CmVar004 has lower growth rate vitro induces only 20% C57BL/6 oviducts versus 83.3%...
Chlamydia pecorum is a globally recognised pathogen of livestock and koalas. To date, comparative genomics C. strains from sheep, cattle koalas has revealed that only single nucleotide polymorphisms (SNPs) limited number pseudogenes appear to contribute the genetic diversity this pathogen. No chlamydial plasmid been detected in these despite its ubiquitous presence almost all other species. Genomic analyses have not previously included porcine hosts. We sequenced genome three isolates pigs...