A5101987321- Multiple Myeloma Research and Treatments
- Chronic Lymphocytic Leukemia Research
- Peptidase Inhibition and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Lymphoma Diagnosis and Treatment
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Antimicrobial Resistance in Staphylococcus
- Protein Degradation and Inhibitors
- Cancer therapeutics and mechanisms
- Cancer-related molecular mechanisms research
- Viral-associated cancers and disorders
- Synthesis and Biological Evaluation
- Liver Disease Diagnosis and Treatment
- Cancer Treatment and Pharmacology
- Streptococcal Infections and Treatments
- CAR-T cell therapy research
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
- Traditional Chinese Medicine Analysis
- RNA modifications and cancer
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Chromosomal and Genetic Variations
- Oral microbiology and periodontitis research
Janssen (United States)
2016-2025
Changsha Medical University
2020-2025
Zhejiang Chinese Medical University
2022-2025
Nanjing Forestry University
2025
Hunan University of Traditional Chinese Medicine
2018-2025
Affiliated Hospital of Southwest Medical University
2022-2025
Shanghai Clinical Research Center
2022-2025
Shenzhen Bao'an District People's Hospital
2024
Shenzhen University Health Science Center
2022-2024
Shenzhen University
2022-2024
Next-generation sequencing technologies are revolutionizing human genomics, promising to yield draft genomes cheaply and quickly. One such technology has now been used analyse much of the genetic code a single individual — who happens be James D. Watson. The procedure, which involves no cloning genomic DNA, makes use latest 454 parallel instrument. sequence cost less than US$1 million (and mere two months) produce, compared approximately US$100 reported for Craig Venter's genome by...
Daratumumab, a human IgGκ monoclonal antibody that targets CD38, induces direct and indirect antimyeloma activity has shown substantial efficacy as monotherapy in heavily pretreated patients with multiple myeloma, well combination bortezomib newly diagnosed myeloma.In this phase 3 trial, we randomly assigned 498 relapsed or refractory myeloma to receive (1.3 mg per square meter of body-surface area) dexamethasone (20 mg) alone (control group) daratumumab (16 kilogram body weight)...
The rhesus macaque (Macaca mulatta) is an abundant primate species that diverged from the ancestors of Homo sapiens about 25 million years ago. Because they are genetically and physiologically similar to humans, monkeys most widely used nonhuman in basic applied biomedical research. We determined genome sequence Indian-origin Macaca mulatta female compared data with chimpanzees humans reveal structure ancestral genomes identify evidence for positive selection lineage-specific expansions...
Daratumumab showed promising efficacy alone and with lenalidomide dexamethasone in a phase 1–2 study involving patients relapsed or refractory multiple myeloma.
Many genomes have been sequenced to high-quality draft status using Sanger capillary electrophoresis and/or newer short-read sequence data and whole genome assembly techniques. However, even the best contain gaps other imperfections due limitations in input techniques used build assemblies. Sequencing biases, repetitive genomic features, polymorphism, complicating factors all come together make some regions difficult or impossible assemble. Traditionally, were upgraded "phase 3 finished"...
A survey of genetic diversity cattle suggests two domestication events in Asia and selection by husbandry.
The process of generating raw genome sequence data continues to become cheaper, faster, and more accurate. However, assembly such into high-quality, finished sequences remains challenging. Many tools are available, but they differ greatly in terms their performance (speed, scalability, hardware requirements, acceptance newer read technologies) final output (composition assembled sequence). More importantly, it largely unclear how best assess the quality sequences. Assemblathon competitions...
News from the Inner Tube of Life A major initiative by U.S. National Institutes Health to sequence 900 genomes microorganisms that live on surfaces and orifices human body has established standardized protocols methods for such large-scale reference sequencing. By combining previously accumulated data with new data, Nelson et al. (p. 994 ) present an initial analysis 178 bacterial genomes. The sampling so far barely scratches surface microbial diversity found humans, but work provides...
We demonstrate the use of nematode Caenorhabditis elegans as a facile and inexpensive model host for several Gram-positive human bacterial pathogens. Enterococcus faecalis, Streptococcus pneumoniae , Staphylococcus aureus, but not Bacillus subtilis, faecium or pyogenes kill adult C. elegans. Focusing our studies on enterococcal species, we found that both E. faecalis eggs hatchlings, although only kills adults. In case adults, low inoculum grows to high titer in intestine, resulting...
Enteric dysbiosis plays an essential role in the pathogenesis of alcoholic liver disease (ALD). Detailed characterization alterations gut microbiome is needed for understanding their pathogenic ALD and developing effective therapeutic approaches using probiotic supplementation. Mice were fed liquid Lieber-DeCarli diet without or with alcohol (5% v/v) 6 weeks. A subset mice administered Lactobacillus rhamnosus GG (LGG) from to 8 Indicators intestinal permeability, hepatic steatosis,...
Andrew Foote and colleagues report the whole-genome sequences de novo assemblies of three marine mammal species—the walrus, killer whale manatee—and an improved bottlenose dolphin genome. Their comparative genomic analysis finds evidence parallel evolution across genomes. Marine mammals from different mammalian orders share several phenotypic traits adapted to aquatic environment therefore represent a classic example convergent evolution. To investigate at level, we sequenced performed...
Systemic immunoglobulin light-chain (AL) amyloidosis is characterized by deposition of amyloid fibrils light chains produced clonal CD38+ plasma cells. Daratumumab, a human CD38-targeting antibody, may improve outcomes for this disease. We randomly assigned patients with newly diagnosed AL to receive six cycles bortezomib, cyclophosphamide, and dexamethasone either alone (control group) or subcutaneous daratumumab followed single-agent every 4 weeks up 24 (daratumumab group). The primary end...
More detailed sequence standards that keep up with revolutionary sequencing technologies will aid the research community in evaluating data.
Escherichia coli DH10B was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage properties such as high transformation efficiency and maintenance plasmids. The strain constructed by serial genetic recombination steps, but underlying sequence changes remained unverified. We report complete genomic using reads accumulated from bovine sequencing project at Baylor College Medicine assembled with DNAStar's SeqMan genome assembler. largely...
Daptomycin is a lipopeptide with bactericidal activity that acts on the cell membrane of enterococci and often used off-label to treat patients infected vancomycin-resistant enterococci. However, emergence resistance daptomycin during therapy threatens its usefulness.We performed whole-genome sequencing characterization envelope clinical pair Enterococcus faecalis isolates from blood patient fatal bacteremia; one isolate (S613) was drawn before treatment other (R712) after daptomycin. The...
Abstract Background Enterococcus faecalis has emerged as a major hospital pathogen. To explore its diversity, we sequenced E. strain OG1RF, which is commonly used for molecular manipulation and virulence studies. Results The 2,739,625 base pair chromosome of OG1RF was found to contain approximately 232 kilobases unique this compared V583, the only publicly available strain. Almost no mobile genetic elements were in OG1RF. 64 areas divergence classified into three categories. First, carries...
In POLLUX, daratumumab (D) plus lenalidomide/dexamethasone (Rd) reduced the risk of disease progression or death by 63% and increased overall response rate (ORR) versus Rd in relapsed/refractory multiple myeloma (RRMM). Updated efficacy safety after >3 years follow-up are presented. Patients (N = 569) with ≥1 prior line received (lenalidomide, 25 mg, on Days 1-21 each 28-day cycle; dexamethasone, 40 weekly) ± at approved dosing schedule. Minimal residual (MRD) was assessed next-generation...
This study aims to identify the causative strain of SARS-CoV-2 in a cluster vaccine breakthroughs. Vaccine breakthrough by highly transmissible is risk global public health.
PURPOSE With the initial analysis of POLLUX at a median follow-up 13.5 months, daratumumab in combination with lenalidomide and dexamethasone (D-Rd) significantly prolonged progression-free survival versus (Rd) alone patients relapsed or refractory multiple myeloma (RRMM). We report updated efficacy safety results time final for overall (OS). METHODS was multicenter, randomized, open-label, phase III study during which eligible ≥ 1 line prior therapy were randomly assigned 1:1 to D-Rd Rd...
At the primary analysis of CASTOR (median follow-up, 7.4 months), daratumumab plus bortezomib and dexamethasone (D-Vd) significantly prolonged progression-free survival versus (Vd) alone in relapsed or refractory multiple myeloma (RRMM). We report updated efficacy safety results at final for overall (OS).CASTOR was a multicenter, randomized, open-label, phase III study during which eligible patients with ≥ 1 line prior therapy were randomly assigned to Vd (up eight cycles) without (until...
Abstract Background Transfer RNA-derived fragments (tRFs) are a new class of small non-coding RNAs. Recent studies suggest that tRFs participate in some pathological processes. However, the biological functions and mechanisms non-small cell lung cancer (NSCLC) largely unknown. Methods Differentially expressed were identified by tRF tiRNA sequencing using 9 pairs pre- post-operation plasma from patients with NSCLC. Quantitative real-time PCR (qRT-PCR) fluorescence situ hybridization (FISH)...