A5111168272- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- MicroRNA in disease regulation
- Mycobacterium research and diagnosis
- Air Quality and Health Impacts
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- Amino Acid Enzymes and Metabolism
- Glycosylation and Glycoproteins Research
- Biochemical and Structural Characterization
- Metabolism and Genetic Disorders
- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- Neuroendocrine Tumor Research Advances
- Proteoglycans and glycosaminoglycans research
- Botanical Research and Applications
- Endometriosis Research and Treatment
- Hybrid Renewable Energy Systems
- Economic and Financial Impacts of Cancer
- IL-33, ST2, and ILC Pathways
- Colorectal Cancer Treatments and Studies
- Energy and Environment Impacts
- RNA and protein synthesis mechanisms
- Cancer Immunotherapy and Biomarkers
Tongji University
2023-2025
Tongji Hospital
2023-2025
Nantong University
2024-2025
Shanghai University
2024-2025
Second Military Medical University
2025
Affiliated Hospital of Nantong University
2024-2025
Shanghai Pudong New Area Gongli Hospital
2025
Shenzhen University Health Science Center
2018-2024
Shanghai Sunshine Rehabilitation Center
2024
Shenzhen University
2020-2021
Airborne fine particulate matter (PM2.5) has been associated with lung cancer development and progression in never smokers. However, the molecular mechanisms underlying PM2.5-induced remain largely unknown. The aim of this study was to explore by which PM2.5 regulated carcinogenesis non-small cell (NSCLC).Paralleled ribosome sequencing (Ribo-seq) RNA (RNA-seq) were performed identify PM2.5-associated genes for further study. Quantitative real time-PCR (qRT-PCR), Western blot,...
Abstract Background Transfer RNA-derived fragments (tRFs) are a new class of small non-coding RNAs. Recent studies suggest that tRFs participate in some pathological processes. However, the biological functions and mechanisms non-small cell lung cancer (NSCLC) largely unknown. Methods Differentially expressed were identified by tRF tiRNA sequencing using 9 pairs pre- post-operation plasma from patients with NSCLC. Quantitative real-time PCR (qRT-PCR) fluorescence situ hybridization (FISH)...
Abstract Non-small cell lung cancer (NSCLC) is the leading cause of mortality worldwide. The mechanisms underlying NSCLC tumorigenesis are incompletely understood. Transfer RNA (tRNA) modification emerging as a novel regulatory mechanism for carcinogenesis. However, role tRNA in remains obscure. In this study, HPLC/MS assay was used to quantify levels tissues and cells. tRNA-modifying enzyme genes were identified by comparative genomics validated qRT-PCR analysis. biological functions gene...
Increasing evidence suggest that long non-coding RNAs (lncRNAs) play critical roles in cancers. However, the expression pattern and underlying mechanisms of lncRNAs non-small cell lung cancer (NSCLC) remain incompletely understood. This study aimed to elucidate functions molecular a certain lncRNA NSCLC.LncRNA microarray was performed identify differential expressed between pre- postoperation plasma NSCLC patients. The level candidate tissues, cells determined by quantitative real-time PCR...
BACKGROUND:There are various pathological types of lung cancer, including squamous cell carcinoma and adenocarcinoma. Although both them cancers, there significant differences in diagnosis, pathogenesis, location, imaging, metastasis, treatment. According to the competing endogenous RNA (ceRNA) theory, long non-coding RNAs (lncRNAs) compete with encoding protein genes (mRNAs) connect miRNAs, thus affecting level mRNA.d MATERIAL AND METHODS:First, using t test, we identified mRNAs lncRNAs...
Accumulating evidence has indicated that long non-coding RNAs (lncRNAs) play critical roles in the development and progression of cancers, including esophageal squamous cell carcinoma (ESCC). However, mechanisms lncRNAs ESCC are still incompletely understood therapeutic attempts for vivo targeting cancer-associated lncRNA remain a challenge. By RNA-sequencing analysis, we identified LLNLR-299G3.1 was novel ESCC-associated lncRNA. up-regulated tissues cells promoted proliferation invasion....
We previously found that intravenous injection of extracellular vesicles (EVs) from human adipose tissue–derived stem cells (hADSC) could ameliorate allergic rhinitis (AR) in mice through immunomodulatory effects. In clinical trials, nasal delivery has been an attractive treatment for AR. sought to determine whether there are differences the therapeutic effects between caudal and their combination. treated AR with ADSC-EVs via vein, cavity, or both. After treatment, were re-sensitized...
Background Human adipose tissue-derived stem cells (hADSCs) exert potent immunosuppressive effects in the allogeneic transplantation treatment. In mouse model of allergic rhinitis (AR), ADSCs partially ameliorated AR. However, no study has evaluated potential therapeutic hADSC-derived extracellular vesicles (hADSC-EVs) on Methods Female BALB/c mice were sensitized and challenged with ovalbumin (OVA) to induce One day after last nasal drop, each group received phosphate buffered saline (PBS)...
Peritoneal dissemination frequently develops in patients with ovarian cancer (OC) and is associated recurrence metastasis. However, the cellular components mechanisms supporting OC peritoneal metastasis are poorly understood. To elucidate these, we utilized RNA sequencing to investigate composition function. Insights from transcriptome analyses suggested that cells malignant ascites persisted a quiescent state of low metabolic activity after metastases peritoneum, arrested were reactivated...
Abstract Background Diabetes mellitus (DM) affects up to one-third of breast cancer (BC) patients. Patients with co-existing BC and DM (BC-DM) have worsened prognosis. Nevertheless, the molecular mechanisms orchestrating BC-DM prognosis remain poorly understood. tRNA-derived fragments (tRFs) been shown regulate progression. However, biological role tRFs in has not explored. Methods tRF levels tumor tissues cells were detected by sequencing qRT-PCR. The effects on cell malignancy assessed...
Abstract Fine particulate matter (PM2.5) exposure has been associated with increased incidence and mortality of lung cancer. However, the molecular mechanisms underlying PM2.5 carcinogenicity remain incompletely understood. Here, we identified that suppressed expression tRNA methyltransferase FTSJ1 Am modification level in vitro vivo. downregulation enhanced glycolytic metabolism non-small cell cancer (NSCLC) cells, as indicated by levels lactate, pyruvate, extracellular acidification rate...
e20542 Background: Transfer RNAs (tRNAs) are key molecules participating in protein synthesis. Modifications of nucleosides on tRNA crucial for efficient and accurate synthesis, modification defects frequently associated with disease. However, a comprehensive profile is still lacking NSCLC. Methods: was isolated from total RNA samples by Urea-PAGE electrophoresis 8 tumor tissues paired adjacent normal resected patients 60-90nt band excised purified ethanol precipitation. Analysis nucleoside...
Rationale: Placental-like chondroitin sulfate A (pl-CSA) is known to be exclusively synthesized in multiple cancer tissues and associated with disease severity.Here, we aimed assess whether pl-CSA released into bio-fluids can serve as a biomarker.Methods: novel ELISA was developed analyse content using binding protein an anti-pl-CSA antibody.Immunohistochemical staining of tissue chips used the gold standard control.Results: The method specific sensitive (1.22 μg/ml).The significantly higher...
e20539 Background: tRNA-derived RNA fragments (tRFs) represent a new class of non-coding single-stranded RNAs with 14-35 nt in length. Recent studies suggest that tRFs play important roles cellular processes and are involved multiple cancers. However, rather little is known about the role non-small cell lung cancer (NSCLC), one most common cancers worldwide. Methods: We used RNA-sequencing to compare expression profiles 10 pairs pre- post-operation plasma samples from patients NSCLC....
Abstract Background Accumulating evidence has indicated that long non-coding RNAs (lncRNAs) play critical roles in the development and progression of cancers, including esophageal squamous cell carcinoma (ESCC). However, mechanisms lncRNAs ESCC are still largely unknown therapeutic attempts for vivo targeting disease-associated lncRNA remain a challenge. Methods By RNA-sequencing analysis, we identified LNLR-299G3.1 was novel ESCC-associated lncRNA. Expression level LLNLR-299G3.1 determined...
Abstract BackgroundlncRNAs are dysregulated in many human cancers, including esophageal squamous cell carcinoma (ESCC), and associated cancer development progression. In the current study, we aimed to elucidate biological roles of lncRNA CTD-2017C7.1 ESCC. MethodThe functions were determined vitro vivo . RNA pull-down, MS, RIP, RNA-seq, qRT-PCR assays employed investigate mechanisms CTD-2017C7.1. ResultsCTD-2017C7.1 was up-regulated ESCC tissues cells, with poor clinical outcome....