A5101450039- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Biochemical Acid Research Studies
- Metabolism and Genetic Disorders
- Enzyme Structure and Function
- Alcoholism and Thiamine Deficiency
- Amino Acid Enzymes and Metabolism
- Biofuel production and bioconversion
- Luminescence and Fluorescent Materials
- Organic Light-Emitting Diodes Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Enzyme Production and Characterization
- Organic Electronics and Photovoltaics
- Asymmetric Hydrogenation and Catalysis
- Pharmacogenetics and Drug Metabolism
- Pharmacological Effects of Natural Compounds
- Microbial Metabolites in Food Biotechnology
- Porphyrin and Phthalocyanine Chemistry
- Metalloenzymes and iron-sulfur proteins
- Plant biochemistry and biosynthesis
- Biochemical and Molecular Research
- NF-κB Signaling Pathways
- PARP inhibition in cancer therapy
- Advanced Drug Delivery Systems
- Cyclopropane Reaction Mechanisms
Jiangnan University
2013-2025
Ningbo University
2024-2025
State Key Laboratory of Food Science and Technology
2025
Ministry of Education Science and Technology
2025
Shanghai Ninth People's Hospital
2025
Sichuan University
2023
Rutgers, The State University of New Jersey
2014-2021
Shenyang Pharmaceutical University
2014-2019
Wuxi Institute of Technology
2016
Diaryl ketones are important building blocks for synthesizing pharmaceuticals and generally regarded as "difficult-to-reduce" due to the large steric hindrance of their two bulky aromatic side chains. Alcohol dehydrogenase from Kluyveromyces polyspora (KpADH) has been identified a robust biocatalyst its high conversion diaryl ketone substrate (4-chlorophenyl)(pyridine-2-yl)ketone (CPMK) with moderate R-selectivity 82% ee. To modulate stereoselectivity KpADH, "polarity scanning" strategy was...
A hydroclassified combinatorial saturation mutagenesis (HCSM) strategy was proposed for reshaping the substrate binding pocket by dividing 20 amino acids into four groups based on their hydrophobicity and size. These smart HCSM libraries could significantly reduce screening effort especially simultaneous of three or more residues lacking high throughput methods. Employing strategy, stereoselectivity KpADH, an alcohol dehydrogenase from Kluyveromyces polysporus, efficiently improved to 99.4%...
Biocatalyzed asymmetric reduction of ketones is one the most cost-effective and environmentally friendly approaches for preparing chiral alcohols. However, few natural ketone reductases (KREDs) or alcohol dehydrogenase (ADHs) with high activity stereoselectivity toward bulky–bulky have yet been reported due to strong steric hindrance these substrates. Recent developments in protein engineering led biocatalysts capable producing alcohols efficiency stereoselectivity. This review discusses...
A d-carbamoylase NiHyuC from Nitratireductor indicus was identified with high catalytic activity toward N-carbamoyl-d-tryptophan (3a). To further enhance its efficiency, both random mutagenesis and structure-guided evolution were performed. Variant M4 (D187N/A200N/S207A/R211G) showed a 43-fold increase in efficiency (kcat/Km = 1135.0 min–1 mM–1) 21-fold reduction Km value (0.4 mM) compared WT. Crystal structures of beneficial variants resolved to clarify the evolutionary changes underlying...
Herein are reported unique properties of the human 2-oxoglutarate dehydrogenase multienzyme complex (OGDHc), a rate-limiting enzyme in Krebs (citric acid) cycle. (a) Functionally competent (E1o-h) and dihydrolipoyl succinyltransferase components have been expressed according to kinetic spectroscopic evidence. (b) A stable free radical, consistent with C2-(C2α-hydroxy)-γ-carboxypropylidene thiamin diphosphate (ThDP) cation radical was detected by electron spin resonance upon reaction E1o-h...
We have developed a carbonyl group-dependent colorimetric method for assay of reductases using inexpensive 2,4-dinitrophenylhydrazine (DNPH).
Significance We applied hydrogen–deuterium exchange MS to a study of full-length Escherichia coli 1-deoxy- d -xylulose 5-phosphate synthase (DXPS), key enzyme in the methylerythritol 4-phosphate pathway, provide snapshot its conformational dynamics. The three regions displaying an EX1 (monomolecular) behavior, two which are absent X-ray structure, were shown be dynamic. Open and closed conformations those revealed with no ligand bound; substrate analogue bound, conformation was favored,...
Oral pathogens can produce volatile sulfur compounds (VSCs), which is the main reason for halitosis and indicates risk of periodontitis. High-sensitivity detection exhaled VSCs urgently desired promoting point-of-care testing (POCT) screening However, current methods often require bulky costly instruments, as well professional training, making them impractical widespread detection. Here, a structural color hydrogel naked-eye presented. reduce disulfide bonds within network, leading to...
The human 2-oxoglutaric acid dehydrogenase complex (hOGDHc) plays a pivotal role in the tricarboxylic (TCA) cycle, and its diminished activity is associated with neurodegenerative diseases. hOGDHc comprises three components, hE1o, hE2o, hE3, we recently reported functionally active E1o E2o enabling studies on their assembly. No atomic-resolution structure for hE2o component currently available, so here first studied interactions binary subcomplexes (hE1o–hE2o, hE1o–hE3, hE2o–hE3) to gain...
The regioselective glycosylation and product specificity of hydroxyflavonoid compounds profoundly influences their biological activity stability, offering significant therapeutic potential. However, most cyclodextrin glucosyltransferases (CGTases) inherently lack regioselectivity for flavone glycosylation. Herein, a CGTase from Paenibacillus macerans was engineered enhanced by combining molecular docking analysis saturation mutagenesis strategies. K232L (favoring 4′-and 6-hydroxyflavones)...
Rare ginsenoside Rh1, exhibiting great potential in the food industry, is limited by its natural scarcity. This constraint has driven development of biocatalytic synthesis approaches, yet robust enzymes capable efficient production remain elusive. Here, we employed ancestral sequence reconstruction (ASR) approach to create a thermostable UDP-dependent glycosyltransferase (UGT227) for Rh1 from 20(S)-protopanaxatriol (PPT). UGT227 exhibited enhanced thermostability (t1/2 = 44.2 h at 60 °C) but...
We previously found that intravenous injection of extracellular vesicles (EVs) from human adipose tissue–derived stem cells (hADSC) could ameliorate allergic rhinitis (AR) in mice through immunomodulatory effects. In clinical trials, nasal delivery has been an attractive treatment for AR. sought to determine whether there are differences the therapeutic effects between caudal and their combination. treated AR with ADSC-EVs via vein, cavity, or both. After treatment, were re-sensitized...
Abstract Nicotinamide cofactor biomimetics (NCBs) serve as low-cost alternatives to the expensive NAD(P) + /NAD(P)H, holding significant potential for applications in oxidoreductases. In this study, an alcohol dehydrogenase ( Sp ADH2) from Sphingobium sp. SYK-6 was identified utilization of synthetic NCBs. ADH2 exhibited a catalytic activity 11.55 U/g oxidation syringyl when utilizing para -3-carbamoyl-1-(4-carboxybenzyl)pyridin-1-ium p -BANA ) cofactor. Semi-rational engineering led...
Boron‐doped polycyclic aromatic hydrocarbons (PAHs) have demonstrated exceptional potential in the development of high‐performance organic light‐emitting diodes (OLEDs). In this study, we propose a novel strategy for constructing PAH structures with thermally activated delayed fluorescence (TADF) properties by incorporating boron‐oxygen (B‐O) covalent bonds, as opposed to doping isolated boron atoms. The newly designed multiheteroatom‐doped molecule, BNB‐OPTZ, was synthesized via...