A5068293016- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Streptococcal Infections and Treatments
- Infective Endocarditis Diagnosis and Management
- Bacterial biofilms and quorum sensing
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial Genetics and Biotechnology
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Biochemical and Structural Characterization
- Escherichia coli research studies
- Mycobacterium research and diagnosis
- Bacteriophages and microbial interactions
- Enterobacteriaceae and Cronobacter Research
- Marriage and Sexual Relationships
- Oral microbiology and periodontitis research
- Antibiotic Use and Resistance
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Pneumonia and Respiratory Infections
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Antifungal resistance and susceptibility
- Microbial Metabolism and Applications
The University of Texas Health Science Center at Houston
2015-2024
Texas Medical Center
2022
University of Redlands
2021
CNR de la Résistance aux Antibiotiques
2020
Florida College
2020
University of Florida
2020
The University of Texas MD Anderson Cancer Center
2019
The University of Texas at Austin
2006-2017
Tufts Medical Center
2016-2017
Washington University in St. Louis
2017
Evidence-based guidelines for the management of patients with methicillin-resistant Staphylococcus aureus (MRSA) infections were prepared by an Expert Panel Infectious Diseases Society America (IDSA). The are intended use health care providers who adult and pediatric MRSA infections. discuss a variety clinical syndromes associated disease, including skin soft tissue (SSTI), bacteremia endocarditis, pneumonia, bone joint infections, central nervous system (CNS) Recommendations provided...
To determine which preoperative factors might affect the development of cardiac complications after major noncardiac operations, we prospectively studied 1001 patients over 40 years age. By multivariate discriminant analysis, identified nine independent significant correlates life-threatening and fatal complications: third heart sound or jugular venous distention; myocardial infarction in preceding six months; more than five premature ventricular contractions per minute documented at any...
Evidence-based guidelines for the management of patients with methicillin-resistant Staphylococcus aureus (MRSA) infections were prepared by an Expert Panel Infectious Diseases Society America (IDSA). The are intended use health care providers who adult and pediatric MRSA infections. discuss a variety clinical syndromes associated disease, including skin soft tissue (SSTI), bacteremia endocarditis, pneumonia, bone joint infections, central nervous system (CNS) Recommendations provided...
Drs. Cesar Arias and Barbara Murray write that we have arrived at a point as frightening the preantibiotic era: for patients infected with multidrug-resistant bacteria, there is no magic bullet.
Infections caused by antibiotic-resistant bacteria, especially the "ESKAPE" pathogens, continue to increase in frequency and cause significant morbidity mortality. New antimicrobial agents are greatly needed treat infections gram-negative bacilli (GNB) resistant currently available agents. The Infectious Diseases Society of America (IDSA) continues propose legislative, regulatory, funding solutions this continuing crisis. current report updates status development approval systemic...
We demonstrate the use of nematode Caenorhabditis elegans as a facile and inexpensive model host for several Gram-positive human bacterial pathogens. Enterococcus faecalis, Streptococcus pneumoniae , Staphylococcus aureus, but not Bacillus subtilis, faecium or pyogenes kill adult C. elegans. Focusing our studies on enterococcal species, we found that both E. faecalis eggs hatchlings, although only kills adults. In case adults, low inoculum grows to high titer in intestine, resulting...
Epidemiologic evaluation of enterococci has been limited by the lack a simple and effective method for comparing strains. In this study, we have compared chromosomal restriction endonuclease digestion patterns 27 isolates Enterococcus faecalis from three different locations using pulsed-field electrophoresis large fragments (14 to 1,000 kilobases). All but two generated clear, evaluable pattern with single lysis digestion, remaining were visualized when larger quantity bacteria was used....
In October 1990, Enterococcus faecium that was highly resistant to glycopeptides, penicillins, and aminoglycosides isolated from the peritoneal dialysis fluid a patient in an intensive care unit. Over following 6 months, multiresistant E. organisms were cultures of blood, urine, or surgical wound specimens eight additional patients. Surveillance groin and/or rectal swabs positive for 37 patients four 62 employees at risk. Restriction endonuclease digestion chromosomal DNA outbreak isolates...
Daptomycin is a lipopeptide with bactericidal activity that acts on the cell membrane of enterococci and often used off-label to treat patients infected vancomycin-resistant enterococci. However, emergence resistance daptomycin during therapy threatens its usefulness.We performed whole-genome sequencing characterization envelope clinical pair Enterococcus faecalis isolates from blood patient fatal bacteremia; one isolate (S613) was drawn before treatment other (R712) after daptomycin. The...
ABSTRACT A multilocus sequence typing (MLST) scheme based on seven housekeeping genes was used to investigate the epidemiology and population structure of Enterococcus faecalis . MLST 110 isolates from different sources geographic locations revealed 55 types that grouped into four major clonal complexes (CC2, CC9, CC10, CC21) by use eBURST. Two these complexes, CC2 are particularly fit in hospital environment, as includes previously described BVE complex identified an alternative CC9...
Abstract Background Enterococcus faecalis has emerged as a major hospital pathogen. To explore its diversity, we sequenced E. strain OG1RF, which is commonly used for molecular manipulation and virulence studies. Results The 2,739,625 base pair chromosome of OG1RF was found to contain approximately 232 kilobases unique this compared V583, the only publicly available strain. Almost no mobile genetic elements were in OG1RF. 64 areas divergence classified into three categories. First, carries...
Streptococcus gallolyticus subsp. (Sg) has long been known to have a strong association with colorectal cancer (CRC). This knowledge important clinical implications, and yet little is about the role of Sg in development CRC. Here we demonstrate that promotes human colon cell proliferation manner depends on context, bacterial growth phase direct contact between bacteria cells. In addition, observed increased level β-catenin, c-Myc PCNA cells following incubation Sg. Knockdown or inhibition...
ABSTRACT Three agr -like genes ( fsrA , fsrB and fsrC for Enterococcus faecalis regulator) were found upstream of the previously reported gelatinase gene gelE ) a downstream putative serine protease sprE ; accession number Z12296 OG1RF. The deduced amino acid sequence shows 26% identity 38% similarity to Staphylococcus aureus AgrA (the response regulator accessory system in locus), FsrB 23% 41% S. AgrB, FsrC 36% AgrC sensor transducer Agr system). Northern blot analysis suggested that are...
Endocarditis isolates of Enterococcus faecalis produced biofilm significantly more often than nonendocarditis isolates, and 39% 79 versus 6% 84 strong (P < 0.0001). esp was not required, but its presence associated with higher amounts 0.001). Mutants disrupted in dltA, efaA, ace, lsa, six two-component regulatory systems were largely unaltered, while disruptions epa (encoding enterococcal polysaccharide antigen), atn an autolysin), gelE gelatinase), fsr the E. regulator) [corrected] resulted...
The presence of vancomycin-resistant enterococci (VRE) was looked for in fecal samples from 104 healthy volunteers (3 with hospital exposure), 100 selected hospitalized patients, and various environmental sources (44 commercial chickens, 5 farm-raised 3 turkeys, 2 chicken farm lagoon slurries). Five probiotic preparations were also studied. No VRE vanA or vanB genes isolated the without exposure, sources, preparations. found stools 16% high-risk patients one volunteer contact. All examined...
Journal Article Incidence of Hemolysin, Gelatinase, and Aggregation Substance among Enterococci Isolated from Patients with Endocarditis Other Infections Feces Hospitalized Community-Based Persons Get access Teresa M. Coque, Coque Search for other works by this author on: Oxford Academic PubMed Google Scholar Jan E. Patterson, Patterson James Steckelberg, Steckelberg Barbara Murray Reprints or correspondence: Dr. Murray, University Texas Medical School, Center Infectious Diseases, 6431...
An open reading frame (hylEfm) with homologies to previously described hyaluronidase genes has been identified in nonstool isolates of Enterococcus faecium. E. faecium (n=577) from diverse sources were screened for the presence hylEfm and espEfm, a putative virulence gene associated epidemic strains. The espEfm was roughly twice that hylEfm, but both found primarily vancomycin-resistant cultures obtained patients hospitalized United States. These data suggest specific strains may be enriched...
ABSTRACT Enterococcus faecalis isolates are resistant to clindamycin (CLI) and quinupristin-dalfopristin (Q-D), this is thought be a species characteristic. Disruption of gene ( abc-23 , now designated lsa for “lincosamide streptogramin A resistance”) E. was associated with ≥40-fold decrease in MICs Q-D (to 0.75 μg/ml), CLI 0.12 0.5 dalfopristin (DAL) 4 8 μg/ml) the wild-type parental strain (Q-D MIC, 32 μg/ml; 48 DAL 512 μg/ml). Complementation disruption mutant on shuttle plasmid resulted...