A5084758555- Infant Nutrition and Health
- Gut microbiota and health
- Reproductive tract infections research
- Diet and metabolism studies
- Breastfeeding Practices and Influences
- Probiotics and Fermented Foods
- Child Nutrition and Water Access
- Birth, Development, and Health
- Reproductive System and Pregnancy
- Pelvic floor disorders treatments
- Fatty Acid Research and Health
- Digestive system and related health
- Urinary Tract Infections Management
- Neonatal Respiratory Health Research
- Reproductive Physiology in Livestock
- Extracellular vesicles in disease
- Metabolomics and Mass Spectrometry Studies
- Nutrition, Genetics, and Disease
- Phytoestrogen effects and research
- Cell death mechanisms and regulation
- Diabetes and associated disorders
- Pediatric health and respiratory diseases
- Infant Health and Development
- Bioactive Compounds and Antitumor Agents
- MicroRNA in disease regulation
Arkansas Children's Nutrition Center
2016-2025
University of Arkansas for Medical Sciences
2014-2024
United States Department of Agriculture
2022-2024
Agricultural Research Service
2022-2024
Arkansas Children's Hospital
2012-2021
University of Arkansas Medical Center
2019
University of Nevada, Las Vegas
2005-2009
Cancer Research Center
2006-2008
Abstract IFN-β has been implicated as an effector of oviduct pathology resulting from genital chlamydial infection in the mouse model. In this study, we investigated role cytosolic DNA and engagement sensors expression during infection. We determined that three-prime repair exonuclease-1, a host 3′ to 5′ exonuclease, reduced significantly using small interfering RNA gene knockout fibroblasts, implicating ligand for response. The sensor cyclic GMP–AMP synthase (cGAS) shown bind generate...
The mechanism by which chlamydiae persist in vivo remains undefined; however, most animals the gastrointestinal tract (GI) and are transmitted via fecal–oral route. Oral infection of mice with Chlamydia muridarum was previously shown to establish a long-term persistent GI tract. In this study, BALB/c, DBA/2, C57Bl/6 mice, infected orally C. , were cecum for as long 100 days absence pathology. primary target tissue although large intestine also animals. A strong serum IgG cecal IgA antibody...
The gut microbiota of breast-fed and formula-fed infants differ significantly, as do the risks for allergies, dysfunction, upper respiratory tract infections. connections between breast milk, various formulas, profiles bacteria to these childhood illnesses, well mechanisms underlying effects, are not understood. We investigated distal colon by 16S RNA amplicon sequencing, morphology histomorphometry, immune response cytokine expression, tryptophan metabolism in a pig model which piglets were...
Neonatal diet has a large influence on child health and might modulate changes in fecal microbiota metabolites. The aim is to investigate metabolites at different ages infants who were breastfed (BF), received dairy-based milk formula (MF), or soy-based (SF). Fecal samples collected 3 (n = 16, 12, 14, respectively), 6 20, 19, 15, 9 11, 12 mo respectively) for BF, MF, SF infants. Infants that until switched considered as no longer breastfeeding mo. Microbiota data obtained using 16S ribosomal...
Abstract IL-1β has been implicated in the development of oviduct pathology during Chlamydia muridarum genital infection mouse model. The goal this study was to characterize role IL-1 signaling and inflammasome-activation pathways chlamydial infection. Compared with control mice, IL-1R–deficient mice displayed delayed clearance increased colonization. Consistent for clearance, deficient IL-1R antagonist cleared at a faster rate. Despite infection, had significantly reduced pathology, which...
ABSTRACT It is not currently possible to predict the probability of whether a woman with chlamydial genital infection will develop pelvic inflammatory disease (PID). To determine if specific biomarkers may be associated distinct pathotypes, we utilized two Chlamydia muridarum variants ( C. Var001 [CmVar001] and CmVar004) that differ in their abilities elicit upper tract pathology mouse model. CmVar004 has lower growth rate vitro induces only 20% C57BL/6 oviducts versus 83.3%...
Breastfeeding is associated with a variety of positive health outcomes in children and recommended exclusively for the first 6 months life; however, 50–70 % infants US are formula-fed. To test hypothesis that immune system development function neonates significantly influenced by diet, 2-day old piglets were fed soy or milk formula (n = 6/group/gender) until day 21 compared to sow-fed group 6/gender). Histomorphometric analyses ileum, jejunum Peyer's patches carried out, determine...
Human milk harbors complex carbohydrates, including human oligosaccharides (HMOs), the third most abundant component after lactose and lipids. HMOs have been shown to impact intestinal microbiota, modulate immune response, prevent pathogenic bacterial binding by serving as decoy receptors. However, direct effect of on function immunity remains be elucidated. To address this knowledge gap, 21-day-old germ-free mice (C57BI/6) were orally gavaged with 15 mg/day pooled for 7 or 14 days...
In recent years, studies with plant compounds have shown both chemotherapeutic and chemopreventive properties. This study stress hormones (jasmonates) showed growth inhibitory effects in breast cancer cells. cis-Jasmone methyl jasmonate (MJ) inhibited the long-term proliferation of MDA-MB-435 MCF-7 Cell cycle analysis G0/G1 S-phase arrest increasing apoptotic population. Cellular signaling MJ decreased membrane fluidity activation extrinsic intrinsic pathways. Specifically pathway increased...
ABSTRACT Evidence from animal studies suggests that chlamydiae may persist in the gastrointestinal tract (GI) and be a reservoir for reinfection of genital tract. We hypothesize there differential susceptibility organisms GI tracts. To determine effect azithromycin on persistent chlamydial gut infection, C57BL/6 BALB/c mice were infected orally genitally treated with (Az) (20, 40, or 80 mg/kg body weight), numbers determined cervix cecal tissues. The Az concentration cecum was measured by...
C hlamydia spp. are obligate, intracellular bacteria that infect humans and animals. Research on these important pathogens has been hindered due to a paucity of genetic tools. We recently adapted group II intron (GII) mutagenesis platform for creation ampicillin-selectable gene insertions in C. trachomatis L2. The aims this study were: (1) assess the stability intron-insertion an vivo infection model gauge efficacy tool long term animal studies (2) expand upon utility method by validating...
Single-dose azithromycin is recommended over multi-dose doxycycline as treatment for chlamydial infection. However, even with imperfect adherence, more effective in treating genital and rectal Recently, it has been suggested that autoinoculation from the rectum to genitals may be a source of persistent infection women. We estimated impact have on effectiveness. estimate effectiveness using simple mathematical model, incorporating data efficacy recent meta-analyses, prevalence women When...
Limited data are available regarding the balance of risks and benefits from human milk and/or breastfeeding during following maternal infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
Maternal diet influences the milk composition, yet little information is available on impact of maternal miRNAs expression. Further, association human to and microbiota not explored. In addition, role infant gut microbiota, growth development has been investigated.
ABSTRACT Not all women infected with chlamydiae develop upper genital tract disease, but the reason(s) for this remains undefined. Host genetics and hormonal changes associated menstrual cycle are possible explanations variable infection outcomes. It is also that disease severity depends on virulence of chlamydial inoculum. likely inoculum contains multiple genetic variants, differing in virulence. If virulent variants dominate, then individual more to severe disease. Based our previous...
The gut microbiome has the potential to create or modify xenometabolites (i.e., nonhost-derived metabolites) through de novo synthesis modification of exogenous and endogenous compounds. While there are isolated examples influencing host health disease, wide-scale characterization these metabolites remains limited. We developed a metabolomics platform ("XenoScan") using liquid chromatography-mass spectrometry characterize range known suspected their derivatives. This assay currently applies...