A5089450547- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Toxin Mechanisms and Immunotoxins
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Probiotics and Fermented Foods
- Barrier Structure and Function Studies
- Viral gastroenteritis research and epidemiology
- Botulinum Toxin and Related Neurological Disorders
- Bacterial Identification and Susceptibility Testing
- Helicobacter pylori-related gastroenterology studies
- Microscopic Colitis
- Bacterial Genetics and Biotechnology
- Pharmacological Effects of Natural Compounds
- Bacterial biofilms and quorum sensing
- Gastrointestinal motility and disorders
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Transgenic Plants and Applications
- Diphtheria, Corynebacterium, and Tetanus
- Mycobacterium research and diagnosis
- Microbial Inactivation Methods
- Enterobacteriaceae and Cronobacter Research
- Caveolin-1 and cellular processes
- Plant-based Medicinal Research
University of Pittsburgh
2016-2025
Oslo University Hospital
2017
Universität Ulm
2017
University of Arizona
1998-2016
Australian Research Council
2006-2015
Monash University
2006-2015
Brigham and Women's Hospital
2006-2010
Dana-Farber Brigham Cancer Center
2010
Dana-Farber Cancer Institute
2010
The University of Texas MD Anderson Cancer Center
2010
Clostridium perfringens is a Gram-positive, anaerobic spore-forming bacterium commonly found in soil, sediments, and the human gastrointestinal tract. C. responsible for wide spectrum of disease, including food poisoning, gas gangrene (clostridial myonecrosis), enteritis necroticans, non-foodborne infections. The complete genome sequences strain ATCC 13124, isolate species type strain, enterotoxin-producing poisoning SM101, were determined compared with published 13 genome. Comparison three...
Clostridium perfringens is an extremely versatile pathogen of humans and livestock, causing wound infections like gas gangrene (clostridial myonecrosis), enteritis/enterocolitis (including one the most common human food-borne illnesses), enterotoxemia (where toxins produced in intestine are absorbed damage distant organs such as brain). The virulence this Gram-positive, spore-forming, anaerobe largely attributable to its copious toxin production; diverse actions roles infection these now...
Previous epidemiological studies have implicated Clostridium perfringens enterotoxin (CPE) as a virulence factor in the pathogenesis of several gastrointestinal (GI) illnesses caused by C. type A isolates, including food poisoning and non‐food‐borne GI illnesses, such antibiotic‐associated diarrhoea sporadic diarrhoea. To further evaluate importance CPE these diseases, allelic exchange was used to construct cpe knock‐out mutants both SM101 (a derivative isolate carrying chromosomal gene)...
Clostridium perfringens enterotoxin (CPE) is an important sporulation-associated virulence factor in several illnesses of humans and domestic animals, including C. type A food poisoning. Therefore, the ability to determine enterotoxigenicity or fecal isolates with simple, rapid assays should be helpful for epidemiologic investigations. In this study, Western immunoblotting (to detect CPE production vitro) was compared PCR digoxigenin-labeled probe all part cpe gene) as a method determining...
Summary Clostridium perfringens type C isolates, which cause enteritis necroticans in humans and enterotoxaemias of domestic animals, typically produce (at minimum) beta toxin (CPB), alpha (CPA) perfringolysin O (PFO) during log‐phase growth. To assist development improved vaccines therapeutics, we evaluated the contribution these three toxins to intestinal virulence disease isolate CN3685. Similar natural infection, vegetative cultures wild‐type CN3685 caused haemorrhagic necrotizing rabbit...
Summary Clostridium perfringens type A isolates carrying an enterotoxin ( cpe ) gene are important cause of human gastrointestinal diseases, including food poisoning, antibiotic‐associated diarrhoea (AAD) and sporadic (SD). Using polymerase chain reaction (PCR), the current study determined that cpb2 encoding recently discovered beta2 toxin is present in <15% poisoning isolates, which typically carry a chromosomal gene. However, >75% AAD/SD usually plasmid gene, tested + by PCR....
Clostridium perfringens is a gram-positive anaerobic rod that classified into 5 toxinotypes (A, B, C, D, and E) according to the production of 4 major toxins, namely alpha (CPA), beta (CPB), epsilon (ETX) iota (ITX).However, this microorganism can produce up 16 toxins in various combinations, including lethal such as perfringolysin O (PFO), enterotoxin (CPE), beta2 toxin (CPB2).Most diseases caused by are mediated one or more these toxins.The role CPA intestinal disease mammals controversial...
The pathogenesis of avian necrotic enteritis involves NetB, a pore-forming toxin produced by virulent isolates Clostridium perfringens type A. To determine the location and mobility netB structural gene, we examined derivative tetracycline-resistant strain EHE-NE18, in which was insertionally inactivated chloramphenicol thiamphenicol resistance gene catP. Both tetracycline could be transferred either together or separately to recipient plate matings. separate transconjugants act as donors...
Clostridium perfringens type A strains producing enterotoxin (CPE) cause one of the most common bacterial food-borne illnesses, as well many cases non-food-borne human gastrointestinal disease. Recent studies have shown that an Agr-like quorum-sensing system controls production chromosomally encoded alpha-toxin and perfringolysin O by C. perfringens, sporulation botulinum sporogenes. The current study explored whether also regulates two plasmid-encoded toxins (CPE beta2 toxin) may contribute...
ABSTRACT Clostridium perfringens type D causes disease in sheep, goats, and other ruminants. Type isolates produce, at minimum, alpha epsilon (ETX) toxins, but some express up to five different raising questions about which toxins are necessary for the virulence of these bacteria. We evaluated contribution ETX C. pathogenicity an intraduodenal challenge model mice using a virulent wild-type strain (WT), isogenic null mutant ( etx mutant), where mutation has been reversed complemented). All...
Multiple Sclerosis (MS) is a complex disease of the CNS thought to require an environmental trigger. Gut dysbiosis common in MS, but specifically causative species are unknown. To address this knowledge gap, we used sensitive and quantitative PCR detection show that people with MS were more likely harbor greater abundance epsilon toxin (ETX)-producing strains C. perfringens within their gut microbiomes compared healthy controls (HC). patient-derived isolates produced functional ETX had...
ABSTRACT Clostridium perfringens enterotoxin (CPE) is an important virulence factor for both C. type A food poisoning and several non-food-borne human gastrointestinal diseases. Recent studies have indicated that isolates associated with carry a chromosomal cpe gene, while disease plasmid gene. However, no explanation has been provided the strong associations between certain genotypes particular CPE-associated Since usually involves cooked meat products, we hypothesized are so strongly...
ABSTRACT Clostridium perfringens enterotoxin (CPE) is responsible for the diarrheal and cramping symptoms of human C. type A food poisoning. CPE-producing isolates have also recently been associated with several non-food-borne gastrointestinal (GI) illnesses, including antibiotic-associated diarrhea sporadic diarrhea. The current study has used restriction fragment length polymorphism (RFLP) pulsed-field gel electrophoresis (PFGE) analyses to compare genotypes 43 cpe -positive obtained from...
ABSTRACT Currently there is only limited understanding of the reservoirs for Clostridium perfringens type A food poisoning. recent survey (Y.-T. Lin and R. Labbe, Appl. Environ. Microbiol. 69: 1642-1646, 2003) non-outbreak American retail foods did not identify presence a single C. isolate carrying enterotoxin gene ( cpe ) necessary causing The present study revisited this issue, using revised methodology sampling strategies. In our survey, -positive isolates were detected in ∼1.4% ∼900...
ABSTRACT Clostridium perfringens type A isolates producing enterotoxin (CPE) are an important cause of food poisoning and non-food-borne human gastrointestinal (GI) diseases, including antibiotic-associated diarrhea (AAD). Recent studies suggest that C. is caused by carrying a chromosomal cpe gene, while CPE-associated GI such as AAD, plasmid isolates. Those putative relationships, obtained predominantly with European isolates, were tested in the current study examining 34 -positive, fecal...
ABSTRACT Although Clostridium perfringens is recognized as an important cause of clostridial enteric diseases, only limited knowledge exists concerning the association particular C. toxinotypes (type A to E) with gastrointestinal (GI) diseases in domestic animals. Some isolates also produce newly discovered beta2-toxin (CPB2). Recent epidemiological studies suggested that carrying gene encoding CPB2 ( cpb2 ) are strongly associated GI animals, including necrotic enteritis piglets and...
A complete copy of the gene (cpe) encoding Clostridium perfringens enterotoxin (CPE), an important virulence factor involved in C. food poisoning and other gastrointestinal illnesses, has been cloned, sequenced, expressed Escherichia coli. The cpe was shown to encode a 319-amino-acid polypeptide with deduced molecular weight 35,317. There no consensus sequence for typical signal peptide present 5' region cpe. Cell lysates from recombinant cpe-positive E. coli were by quantitative immunoblot...