Chlamydia psittaci is an avian pathogen and zoonotic agent of atypical pneumonia. The most pathogenic C. strains cluster into the 6BC clade, predicted to have recently emerged globally. Exposure infected parrots a risk factor with limited evidence also indirect exposure risk. Genome sequencing was performed on six Australian human single strain isolated over 9 year period. Only one five patients had explicit psittacine contact. Genomics analyses revealed that are remarkably similar,...

Chlamydia pecorum is a significant pathogen of domestic livestock and wildlife. We have developed C. pecorum-specific multilocus sequence analysis (MLSA) scheme to examine the genetic diversity relationships between Australian sheep, cattle, koala isolates. An MLSA seven concatenated housekeeping gene fragments was performed using 35 isolates, including 18 isolates (11 one cow, six U.S. isolates) 17 Phylogenetic analyses showed that formed distinct clade, with limited clustering from sheep....

Chlamydia psittaci is an avian pathogen capable of spill-over infections to humans. A parrot C. strain was recently detected in equine reproductive loss case associated with a subsequent cluster human infections. In this study, we screened for cases reported regional New South Wales, Australia during the 2016 foaling season. specific-PCR screening foetal and placental tissue samples from abortion (n = 161) foals compromised health status 38) revealed positivity 21.1% 23.7%, respectively....

Chlamydia pecorum is a globally recognised pathogen of livestock and koalas. To date, comparative genomics C. strains from sheep, cattle koalas has revealed that only single nucleotide polymorphisms (SNPs) limited number pseudogenes appear to contribute the genetic diversity this pathogen. No chlamydial plasmid been detected in these despite its ubiquitous presence almost all other species. Genomic analyses have not previously included porcine hosts. We sequenced genome three isolates pigs...

Chlamydia psittaci and pecorum are important veterinary pathogens, with the former also being responsible for zoonoses, latter adversely affecting koala populations in Australia livestock globally. The rapid detection of these organisms is still challenging, particularly at point-of-care (POC). In present study, we developed evaluated rapid, sensitive robust C. psittaci-specific pecorum-specific Loop Mediated Isothermal Amplification (LAMP) assays pathogens.The LAMP assays, performed a Genie...

Chlamydia psittaci is a globally distributed veterinary pathogen with zoonotic potential. Although C. infections have been reported in various hosts, isolation and culture of challenging, hampering efforts to produce contemporary global genomes. This particularly evident the lack avian genomes from Australia New Zealand. In this study, we used culture-independent probe-based whole-genome sequencing expand genome catalogue. Here, provide new two pigeons, six psittacines, novel hosts such as...

ABSTRACT Chlamydia pecorum is an important global pathogen of livestock, and it also a significant threat to the long-term survival Australia's koala populations. This study employed culture-independent DNA capture approach sequence C. genomes directly from clinical swab samples collected koalas with chlamydial disease as well sheep arthritis conjunctivitis. Investigations into single-nucleotide polymorphisms within each revealed that portion reads in sample belonged separate strains,...

Chlamydia pecorum is an important pathogen of domesticated livestock including sheep, cattle and pigs. This also a key factor in the decline koala Australia. We sequenced genomes three C. strains, isolated from urogenital tracts conjunctiva diseased koalas. The genome VR629 (IPA) strain, sheep with polyarthritis, was sequenced. Comparisons draft against complete isolates revealed that these strains have conserved gene content order, sharing nucleotide sequence similarity > 98%. Single...

Feral pigeons, common wood pigeons and Eurasian collared doves are the most representatives of Columbidae family in Switzerland mostly present highly populated, urban areas. Pigeons may carry various members obligate intracellular Chlamydiaceae family, particularly Chlamydia (C.) psittaci, a known zoonotic agent, C. avium. The objective study was to identify infection rates free-roaming for different species with overall aim assess risk pose public health. In this study, 431 (323 feral 34...

Despite its global recognition as a ruminant pathogen, cases of Chlamydia pecorum infection in Australian livestock are poorly documented. In this report, C. specific Multi Locus Sequence Analysis scheme was used to characterise the strains implicated two sporadic bovine encephalomyelitis confirmed by necropsy, histopathology and immunohistochemistry. This report provides first molecular evidence for presence mixed infections cattle. Affected animals were markedly depressed, dehydrated blind...

We assessed the effects of two different single-dose anti-Chlamydia pecorum (C. pecorum) vaccines (containing either Major Outer Membrane Protein (3MOMP) or Polymorphic (Pmp) as antigens) on immune response a group wild koalas. Both elicited systemic humoral seen by production anti-chlamydial IgG antibodies in more than 90% vaccinated A mucosal was also observed, with an increase Chlamydia-specific and/or IgA some koalas post-vaccination. cell-mediated measured cytokines IFN-γ and IL-17 To...

Arthritis is an economically significant disease in lambs and usually the result of a bacterial infection. One known agents this Chlamydia pecorum, globally recognised livestock pathogen associated with several diseases sheep, cattle other hosts. Relatively little published information available on clinical, diagnostic pathologic features C. pecorum arthritis hindering efforts to enhance our understanding disease. In case series, combination standard testing used routinely by veterinarians,...

Abstract Chlamydia pecorum is an established and prevalent infection that produces severe clinical disease in many koala populations, contributing to dramatic population declines. In wild South Australian C . occurrence distribution unknown. Here, pecorum- specific real-time quantitative PCR (qPCR) was applied ocular urogenital swabs from targeted surveys of koalas the mainland Mount Lofty Ranges (MLR) (n = 75) Kangaroo Island (KI) 170) populations. Historical data 13,081 KI (1997–2018)...

Abstract Chlamydial disease control is increasingly utilised as a management tool to stabilise declining koala populations, and yet we have limited understanding of the factors that contribute progression. To examine impact host pathogen genetics, selected two geographically separated south east Queensland differentially affected by chlamydial disease, analysed major histocompatibility complex (MHC) genes, circulating strains Chlamydia pecorum retrovirus (KoRV) subtypes in longitudinally...

Abstract Lamb survival is an important welfare and productivity issue for sheep industries worldwide. Lower lamb has been reported primiparous ewes, but the causes of this are not well studied. The aim study was to determine perinatal deaths lambs born ewes in Western Australia, identify if infectious diseases implicated. mortality from birth marking were determined 11 ewe flocks on 10 farms Australia. averaged 14% single-born 26% multiple-born lambs. necropsies ( n = 298) identified...

Chlamydia psittaci is an avian pathogen with zoonotic potential. In Australia, C. has been well reported as a cause of reproductive loss in mares which subsequently have the source infection and illness some in-contact humans. To date, molecular typing studies describe predominant clonal sequence type (ST)24 strains horse, psittacine, human infections. We sought to assess clonality between ST24 emergence equine comprehensive genomics approach. used culture-independent probe-based metagenomic...

Chlamydia pecorum is a widespread veterinary chlamydial species causing endemic infections in livestock, such as ruminants and pigs, globally. However, there limited contemporary knowledge on infecting strain diversity various hosts. This study aimed to evaluate the genetic of C. strains Swiss livestock through genotyping phylogenetic analyses comparison global population, while also assessing for plasmid carriage. A total 263 positive samples from clinically healthy ruminant pig herds...

To overcome shortcomings in discriminating Chlamydia pecorum strains infecting the koala (Phascolarctos cinereus) at local level, we developed a novel genotyping scheme for this pathogen to inform management fine-scale subpopulation level. We applied two geographically distinct populations New South Wales, Australia: Liverpool Plains and Southern Highlands South-west Sydney (SHSWS). Our method provides greater resolution than traditional multi-locus sequence typing, can be used monitor...

Chlamydia psittaci is an obligate, intracellular, bacterial pathogen generally associated with clinical and subclinical infection of birds. It a zoonotic in humans causing psittacosis serious respiratory disease reported to cause animals including cattle, sheep horses. Although there have been sporadic reports due C. horses since the last century, from Australia over decade highlighted potential transfer. Epizootics abortions stillbirths mares neonatal disease, termed equine (EP), both...

Chlamydia pecorum is a significant cause of morbidity and mortality in koalas major contributor to population decline. Due its crucial role vaccine development use as strain typing tool, multiple studies have investigated the prevalence diversity C. outer membrane protein A (MOMP), encoded by ompA. This prior work has shown that ompA genotypes vary across geographical regions, with identified Eastern Australia. Despite these earlier studies, our understanding distribution MOMP remains...