A5041575100- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Gut microbiota and health
- Reproductive tract infections research
- Bacterial Identification and Susceptibility Testing
- Mycobacterium research and diagnosis
- Rabies epidemiology and control
- Antibiotics Pharmacokinetics and Efficacy
- Herpesvirus Infections and Treatments
- Zoonotic diseases and public health
- Burkholderia infections and melioidosis
- Wildlife Ecology and Conservation
- Antimicrobial Peptides and Activities
- Syphilis Diagnosis and Treatment
- Viral Infections and Vectors
- Microbial infections and disease research
- Animal Behavior and Welfare Studies
- Pediatric health and respiratory diseases
- Milk Quality and Mastitis in Dairy Cows
- Data-Driven Disease Surveillance
- Genomics and Phylogenetic Studies
University of the Sunshine Coast
2017-2024
Sunshine Coast University Hospital
2019-2024
The koala, the only extant species of marsupial family Phascolarctidae, is classified as 'vulnerable' due to habitat loss and widespread disease. We sequenced koala genome, producing a complete contiguous reference including centromeres. reveal that koala's ability detoxify eucalypt foliage may be expansions within cytochrome P450 gene family, its smell, taste moderate ingestion plant secondary metabolites in vomeronasal receptors. characterized novel lactation proteins protect young pouch...
Chlamydia psittaci and pecorum are important veterinary pathogens, with the former also being responsible for zoonoses, latter adversely affecting koala populations in Australia livestock globally. The rapid detection of these organisms is still challenging, particularly at point-of-care (POC). In present study, we developed evaluated rapid, sensitive robust C. psittaci-specific pecorum-specific Loop Mediated Isothermal Amplification (LAMP) assays pathogens.The LAMP assays, performed a Genie...
BackgroundAntimicrobial resistance (AMR) poses a major threat to human health. Whole-genome sequencing holds great potential for AMR identification; however, there remain gaps in accurately and comprehensively detecting across the spectrum of AMR-conferring determinants pathogens.MethodsUsing 16 wild-type Burkholderia pseudomallei 25 with acquired AMR, we first assessed performance existing software (ARIBA, CARD, ResFinder, AMRFinderPlus) clinically relevant this pathogen. B. was chosen due...
Abstract Background Antimicrobial resistance (AMR) is an intensifying threat that requires urgent mitigation to avoid a post-antibiotic era. Pseudomonas aeruginosa represents one of the greatest AMR concerns due increasing multi- and pan-drug rates. Shotgun sequencing gaining traction for in silico profiling its unambiguity transferability; however, accurate comprehensive prediction from P. genomes remains unsolved problem. Methods We first curated most database yet known variants. Next, we...
The rise of antimicrobial-resistant (AMR) bacteria is a global health emergency. One critical facet tackling this epidemic more rapid AMR diagnosis in serious multidrug-resistant pathogens like Pseudomonas aeruginosa. Here, we designed and then validated two multiplex quantitative real-time PCR (qPCR) assays to simultaneously detect differential expression the resistance-nodulation-division efflux pumps MexAB-OprM, MexCD-OprJ, MexEF-OprN, MexXY-OprM, AmpC β-lactamase, porin OprD, which are...
Koalas (Phascolarctos cinereus) are native Australian marsupials whose populations in decline from a range of threats. Infectious diseases caused by the bacterium Chlamydia pecorum and other pathogens particular concern. We analysed 26 poly-A selected RNA-sequencing libraries data set designed to study immune response koalas ocular chlamydial infection. Using virus discovery techniques, we identified coding-complete genome sequence novel picorna-like virus, denoted Burpengary that was most...
Background. Antimicrobial resistance (AMR) is an ever-increasing global health concern. One crucial facet in tackling the AMR epidemic earlier and more accurate diagnosis, particularly dangerous highly multi-drug-resistant ESKAPE pathogen, Pseudomonas aeruginosa.Objectives. We aimed to develop two SYBR Green-based mismatch amplification mutation assays (SYBR-MAMAs) targeting GyrA T83I (gyrA248) D87N, D87Y D87H (gyrA259). Together, these variants cause majority of fluoroquinolone (FQ) P....
Abstract Background Antimicrobial resistance (AMR) is an intensifying threat that requires urgent mitigation to avoid a post-antibiotic era. The ESKAPE pathogen, Pseudomonas aeruginosa , represents one of the greatest AMR concerns due increasing multi- and pan-drug rates. Shotgun sequencing quickly gaining traction for in silico profiling its unambiguity transferability; however, accurate comprehensive prediction from P. genomes remains unsolved problem. Methods We first curated most...
Introduction This study employs bulk RNA sequencing, PCR, and ELISA assays to analyze the pathological factors affecting outcomes of C. pecorum ocular infections in koalas. It investigates immune responses gene expression profiles associated with various stages koala chlamydiosis. Methods A cohort 114 koalas from Queensland, Australia were assessed, 47% displaying clinical signs disease. Animals classified into three cohorts: acute active disease (G1), chronic (G2), inactive (G3), along...
Abstract Background Antimicrobial resistance (AMR) poses a major threat to human health. Whole-genome sequencing holds great potential for AMR identification; however, there remain gaps in comprehensively detecting across the spectrum of AMR-conferring determinants and pathogens. Methods Using 16 wild-type Burkholderia pseudomallei 25 with acquired AMR, we first assessed performance existing software (ARIBA CARD) clinically relevant this pathogen. B. was chosen due limited treatment options,...
Abstract Background Antimicrobial resistance (AMR) is an ever-increasing global health concern. One crucial facet in tackling the AMR epidemic earlier and more accurate diagnosis, particularly dangerous highly multi-drug resistant ESKAPE pathogen, Pseudomonas aeruginosa . Objectives We aimed to develop two SYBR Green-based mismatch amplification mutation assays (SYBR-MAMAs) targeting GyrA T83I ( gyrA 248), D87N, D87Y, D87H 259). Together, these variants cause majority of fluoroquinolone (FQ)...
Koalas (Phascolarctos cinereus) are native Australian marsupials whose populations in decline from a range of threats. Infectious diseases caused by the bacterium Chlamydia pecorum and other pathogens particular concern. We analysed 26 poly-A selected RNA-sequencing libraries data set designed to study immune response koalas ocular chlamydial infection. Using virus discovery techniques, we identified coding-complete genome sequence novel picorna-like virus, denoted Burpengary that was most...
Abstract The rise of antimicrobial-resistant (AMR) bacteria is a global health emergency. One critical facet in tackling this epidemic more rapid AMR diagnosis serious multi-drug resistant pathogens like Pseudomonas aeruginosa . Here, we designed and then validated two multiplex quantitative real-time PCR (qPCR) assays to simultaneously detect differential expression the resistance-nodulation-division efflux pumps MexAB-OprM, MexCD-OprJ, MexEF-OprN, MexXY-OprM, AmpC β-lactamase, porin OprD,...