A5007615856- Burkholderia infections and melioidosis
- Bioeconomy and Sustainability Development
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- Antibiotic Resistance in Bacteria
- Chemical Looping and Thermochemical Processes
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- Bacterial Identification and Susceptibility Testing
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- Toxoplasma gondii Research Studies
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- Diabetes and associated disorders
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- Porphyrin Metabolism and Disorders
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Peter Doherty Institute
2022-2025
The University of Melbourne
2022-2025
Charles Darwin University
2019-2024
Menzies School of Health Research
2013-2024
University of California, Riverside
2024
Native Women's Association of Canada
2023
Royal Darwin Hospital
2021-2022
Cedars-Sinai Medical Center
2021
University of the Sunshine Coast
2021
Children’s Village
2019
Abstract Genomics is a cornerstone of modern pathogen epidemiology yet demonstrating transmission in One Health context challenging, as strains circulate and evolve within between diverse hosts environments. To identify phylogenetic linkages better define relevant measures genomic relatedness context, we collated 5471 Escherichia coli genome sequences from Australia originating humans ( n = 2996), wild animals 870), livestock 649), companion 375), environmental sources 292) food 289)...
Burkholderia pseudomallei is a Gram-negative environmental bacterium that causes melioidosis, potentially life-threatening infectious disease affecting mammals, including humans. Melioidosis symptoms are both protean and diverse, ranging from mild, localized skin infections to more severe often fatal presentations pneumonia, septic shock with multiple internal abscesses occasionally neurological involvement. Several ubiquitous virulence determinants in B. have already been discovered....
Melioidosis, caused by the bacterium Burkholderia pseudomallei, is an uncommon infection that typically associated with exposure to soil and water in tropical subtropical environments. It rarely diagnosed continental United States. Patients melioidosis States commonly report travel regions where endemic. We a cluster of four non-travel-associated cases Georgia, Kansas, Minnesota, Texas. These were same strain B. pseudomallei was linked aromatherapy spray product imported from...
Burkholderia pseudomallei, the causative agent of high-mortality disease melioidosis, is a gram-negative bacterium that naturally resistant to many antibiotics. There no vaccine for and effective eradication reliant on biphasic prolonged antibiotic administration. The carbapenem drug meropenem current gold standard option treating severe melioidosis. Intrinsic B. pseudomallei resistance toward has not yet been documented; however, could conceivably develop over course infection, leading...
The bacterium Burkholderia ubonensis is commonly co-isolated from environmental specimens harbouring the melioidosis pathogen, pseudomallei. B. has been reported in northern Australia and Thailand but not North America, suggesting similar geographic distribution to Unlike most other cepacia complex (Bcc) species, considered non-pathogenic, although its virulence potential tested. Antibiotic resistance ubonensis, particularly towards drugs used treat severe pseudomallei infections, also...
Burkholderia ubonensis is an environmental bacterium belonging to the cepacia complex (Bcc), a group of genetically related organisms that are associated with opportunistic but generally nonfatal infections in healthy individuals. In contrast, near-neighbour species pseudomallei causes melioidosis, disease can be fatal up 95% cases if left untreated. B. frequently misidentified as from soil samples using selective culturing on Ashdown's medium, reflecting both shared niche and morphological...
BackgroundAntimicrobial resistance (AMR) poses a major threat to human health. Whole-genome sequencing holds great potential for AMR identification; however, there remain gaps in accurately and comprehensively detecting across the spectrum of AMR-conferring determinants pathogens.MethodsUsing 16 wild-type Burkholderia pseudomallei 25 with acquired AMR, we first assessed performance existing software (ARIBA, CARD, ResFinder, AMRFinderPlus) clinically relevant this pathogen. B. was chosen due...
Melioidosis is caused by the environmental pathogen Burkholderia pseudomallei. Among 1,331 patients with melioidosis during 1989-2023 in Darwin Prospective Study Australia, we identified 6 locally acquired cases B. pseudomallei sequence type 46. Because of global transmission and expansion endemicity, clinicians should increase awareness melioidosis.
Abstract Melioidosis is a severe disease caused by the environmental bacterium Burkholderia pseudomallei that affects both humans and animals throughout northern Australia, Southeast Asia increasingly globally. While there considerable degree of genetic diversity amongst isolates, B. has robust global biogeographic structure populations are spatially clustered in environment. We examined distribution local spread Darwin, Northern Territory, which highest recorded urban incidence melioidosis...
Abstract Background The autotransporter protein Burkholderia intracellular motility A (BimA) facilitates the entry of pseudomallei into central nervous system (CNS) in mouse models melioidosis. Its role pathogenesis human cases CNS melioidosis is incompletely defined. Methods Consecutive culture-confirmed at 2 sites tropical Australia after 1989 were reviewed. Demographic, clinical, and radiological data patients with recorded. bimA allele (bimABm or bimABp) B. isolated from each patient was...
Abstract Ecological restoration often targets plant community recovery, but success may depend on the recovery of a complex web biotic interactions to maintain biodiversity and promote ecosystem services. Specifically, management that drives resource availability, such as seeding richness provenance, alter species across multiple trophic levels. Using experimentally seeded prairies, we examine three key groups—plants, pollinators goldenrod crab spiders ( Misumena vatia , predators...
ABSTRACT The soil-dwelling bacterium Burkholderia pseudomallei is the causative agent of potentially fatal disease melioidosis. lack a vaccine toward B. means that melioidosis treatment relies on prolonged antibiotic therapy, which can last up to 6 months in duration or longer. Due intrinsic resistance, few antibiotics are effective against . lengthy regimen required increases likelihood resistance development, with subsequent relapse. Doxycycline (DOX) has historically played an important...
The causative agent of melioidosis is the Gram-negative bacterium Burkholderia pseudomallei. Clinical presentations are notably diverse, with host risk factors considered central to progression from infection disease and clinical outcome. Ubiquitous variably present virulence determinants have been described for B pseudomallei, several minority genotypes associated specific presentations. lipopolysaccharide (LPS) O-antigen pseudomallei highly diverse 3 types described. In vitro data suggest...
Melioidosis is endemic in the remote Katherine region of northern Australia. In a population with high rates chronic disease, social inequities, and extreme remoteness, impact melioidosis exacerbated by severe weather events disproportionately affects First Nations Australians. All culture-confirmed cases Australian Top End between 1989–2021 were included study, clinical features epidemiology described. The diversity Burkholderia pseudomallei strains was investigated using genomic...
Cefiderocol is a siderophore cephalosporin designed mainly for treatment of infections caused by β-lactam and multidrug-resistant Gram-negative bacteria. Burkholderia pseudomallei clinical isolates are usually highly cefiderocol susceptible, with in vitro resistance found few isolates. Resistance B. from Australia hitherto uncharacterized mechanism. We show that, like other Gram-negatives, the PiuA outer membrane receptor plays major role nonsusceptibility Malaysia.
Abstract Melioidosis was first identified in Myanmar 1911 but for the last century it has remained largely unreported there. Burkholderia pseudomallei isolated from environment of 2016, confirming continuing endemicity. Recent genomic studies showed that B. originated Australia and spread to Asia, with phylogenetic evidence repeated reintroduction across countries bordered by Mekong River Malay Peninsula. We present whole-genome sequences isolates Myanmar: nine clinical seven environmental...
Burkholderia pseudomallei is the causative agent of high-mortality disease melioidosis. Although melioidosis classified as a tropical disease, rare autochthonous cases have been reported from temperate climatic regions, with uncertainty to whether B. persistent in local environment and specific genetic mechanisms facilitate survival outside tropics. Sporadic occurred valley region (latitude 31.6°S) southwest Western Australia, between 1966 1992. We report new cluster same following high...
In the wet-dry tropics of Northern Australia, drinking water in remote communities is mostly sourced from bores accessing groundwater. Many aquifers contain naturally high levels iron and some are shallow with surface intrusion wet season. Therefore, environmental bacteria such as iron-cycling promoting biofilm formation pipes or opportunistic pathogens can occur these waters. An pathogen endemic to northern Australia Southeast Asia emerging worldwide Burkholderia pseudomallei. It causes...
The Tier 1 select agent Burkholderia pseudomallei is an environmental bacterium that causes melioidosis, a high mortality disease. Variably present genetic markers used to elucidate strain origin, relatedness and virulence in B. include the intracellular motility factor A (bimA) filamentous hemagglutinin 3 (fhaB3) gene variants. Three lipopolysaccharide (LPS) O-antigen types have been described, which vary proportion between Australian Asian isolates. However, it remains unknown if these LPS...
Abstract Since 2005, the range of Burkholderia pseudomallei sequence type 562 (ST562) has expanded in northern Australia. During 2005–2019, ST562 caused melioidosis 61 humans and 3 animals. Cases initially occurred suburbs surrounding a creek before spreading across urban Darwin, Australia nearby island community. In 12% (53/440) cases, proportion that increased during study period. We analyzed 2 clusters cases with epidemiologic links used genomic analysis to identify previously...
Burkholderia cepacia complex (Bcc) and pseudomallei (Bpc) species include pathogens that are typically multidrug resistant. Dominant intrinsic acquired resistance mechanisms efflux mediated by pumps of the resistance-nodulation-cell division (RND) family. From comparative bioinformatic and, in many instances, functional studies, we infer RND pump-based conserved Burkholderia. We propose to use these findings as a foundation for adoption uniform pump nomenclature.