Andrew King
A5001858084- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Genetic diversity and population structure
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- Genomics and Phylogenetic Studies
- Wildlife Ecology and Conservation
- Genomics and Chromatin Dynamics
- Genetic and phenotypic traits in livestock
- RNA modifications and cancer
- Yersinia bacterium, plague, ectoparasites research
- RNA Research and Splicing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomic variations and chromosomal abnormalities
- Immunotherapy and Immune Responses
- Bat Biology and Ecology Studies
- Species Distribution and Climate Change
- Reproductive System and Pregnancy
- Identification and Quantification in Food
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Angiogenesis and VEGF in Cancer
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
King's College Hospital NHS Foundation Trust
2016-2025
King's College London
2024-2025
Australian Museum
2011-2024
Louisiana State University Health Sciences Center New Orleans
2024
Louisiana State University
2024
King's College - North Carolina
2024
Agricultural Research Service
2017
GlaxoSmithKline (United Kingdom)
2003-2014
GlaxoSmithKline (United States)
2002-2011
Johnson & Johnson (Australia)
1999-2009
Knowledge of the complete genomic DNA sequence an organism allows a systematic approach to defining its genetic components. The provides access structures all genes, including those without known function, their control elements, and, by inference, proteins they encode, as well other biologically important sequences. Furthermore, is rich and permanent source information for design further biological studies study evolution through cross-species comparison. power this has been amply...
The koala, the only extant species of marsupial family Phascolarctidae, is classified as 'vulnerable' due to habitat loss and widespread disease. We sequenced koala genome, producing a complete contiguous reference including centromeres. reveal that koala's ability detoxify eucalypt foliage may be expansions within cytochrome P450 gene family, its smell, taste moderate ingestion plant secondary metabolites in vomeronasal receptors. characterized novel lactation proteins protect young pouch...
The finished sequence of human chromosome 20 comprises 59,187,298 base pairs (bp) and represents 99.4% the euchromatic DNA. A single contig 26 megabases (Mb) spans entire short arm, five contigs separated by gaps totalling 320 kb span long arm this metacentric chromosome. An additional 234,339 bp has been determined within pericentromeric region arm. We annotated 727 genes 168 pseudogenes in sequence. About 64% these have a 5' 3' untranslated complete open reading frame. Comparative analysis...
Myelosuppression has been observed with several multikinase angiogenesis inhibitors in clinical studies, although the frequency and severity varies among different agents. Inhibitors targeting vascular endothelial growth factor receptor (VEGFR) often inhibit other kinases, which may contribute to their adverse-event profiles. Kinase selectivity of pazopanib, sorafenib, sunitinib was evaluated a panel 242 kinases. Cellular potency measured using autophosphorylation assays. Effect on human...
A nonpeptidyl small molecule SB 247464, capable of activating granulocyte-colony-stimulating factor (G-CSF) signal transduction pathways, was identified in a high-throughput assay cultured cells. Like G-CSF, 247464 induced tyrosine phosphorylation multiple signaling proteins and stimulated primary murine bone marrow cells to form granulocytic colonies vitro. It also elevated peripheral blood neutrophil counts mice. The extracellular domain the G-CSF receptor required for activity suggesting...
Abstract The virulence of chlamydial infection in wild koalas is highly variable between individuals. Some can be infected (PCR positive) with Chlamydia for long periods but remain asymptomatic, whereas others develop clinical disease. the koala has traditionally been studied without regard to coinfection other pathogens, although are usually subject retrovirus (KoRV). Retroviruses immunosuppressive, and there evidence an immunosuppressive effect KoRV vitro . Originally thought a single...
The koala, Phascolarctos cinereus, is a biologically unique and evolutionarily distinct Australian arboreal marsupial. goal of this study was to sequence the transcriptome from several tissues two geographically separate koalas, create first comprehensive catalog annotated transcripts for species, enabling detailed analysis attributes threatened native marsupial, including infection by koala retrovirus. RNA-Seq data generated range one male female assembled de novo into using Velvet-Oases....
Short interfering RNAs (siRNAs) have been shown to induce immune stimulation through a number of different receptors in range cell types. In primary cells, both TLR7 and TLR8 recognise siRNAs however, despite the identification TLR7/8 stimulatory RNA motifs, complete definitive sequence determinants are yet be elucidated. A total 207 siRNA sequences were screened for human PBMCs. There was significant correlation between U count U-rich strand immunostimulatory activity duplex. Using...
Abstract The koala retrovirus (KoRV) is implicated in several diseases affecting the (Phascolarctos cinereus) . KoRV provirus can be present genome of koalas as an endogenous (present all cells via germline integration) or exogenous responsible for somatic integrations proviral a limited number cells). This ongoing invasion by provides powerful opportunity to assess viral strategies used individual. Analysis high-quality sequence single revealed 133 integration sites. Most contain...
Abstract Urbanization represents the most extreme form of land cover transformation and is expected to restrict dispersal animals, both because structural unsuitability novel habitat, as well through mechanisms associated with human activity, such disturbance. Fragmentation populations by urbanization considered be a significant threat several endangered bird populations, although isolation has seldom been demonstrated genetically. The aim this study was explore utility genetic criteria for...
The capacity for dispersal is an important determinant of a species’ resilience to decline from the serial extinction local populations. Dispersal animals can be difficult measure directly but population genetics provides powerful tool identifying limits. This study used microsatellite markers investigate structuring white-fronted chat (Epthianura albifrons) across its geographic range. Because species threatened in north-eastern part range, primary aim was identify barriers limiting...
Frontotemporal lobar degeneration with neuronal inclusions of the TAR DNA-binding protein 43 (FTLD-TDP) is a fatal neurodegenerative disorder only limited number risk loci identified. We report our comprehensive genome-wide association study as part International FTLD-TDP Whole-Genome Sequencing Consortium, including 985 patients and 3,153 controls compiled from 26 institutions/brain banks in North America, Europe Australia, meta-analysis Dementia-seq cohort. confirm UNC13A strongest overall...
SK&F 107647, a previously described synthetic immunomodulatory peptide, indirectly stimulates bone marrow progenitor cells and phagocytic cells, enhances host defense effector mechanisms in bacterial fungal infection models vivo. In vitro, 107647 induces the production of soluble mediator that augments colony forming cell (CFU-GM) formation presence CSFs. this paper we purified sequenced stromal cell-derived hematopoietic synergistic factors (HSF) secreted from both murine human lines...
A hammerhead ribozyme retrovial construct, denoted RRz2, targeting the coding region of human immunodeficiency virus type 1 (HIV-1) tat gene, has shown itself to be effective in a range test systems. Inhibition replication HIV-1 IIIB and primary drug-resistant strains pooled transduced CEMT4 cells was consistently found more than 80% compared with control-vector cells, whereas mutant RRz2 gave approximately 45% inhibition. multiple passage assay showed absence emergence mutations within...