CtIP plays an important role in homologous recombination (HR)-mediated DNA double-stranded break (DSB) repair and interacts with Nbs1 BRCA1, which are linked to Nijmegen breakage syndrome (NBS) familial breast cancer, respectively. We identified new CDK phosphorylation sites on found that of these newly induces association the N-terminus FHA BRCT domains Nbs1. further showed CDK-dependent events a prerequisite for ATM phosphorylate upon damage, is end resection activate HR by promoting...

MAPK phosphatase-1 (MKP-1) is an archetypical member of the dual-specificity phosphatase family that deactivates MAPKs. Induction MKP-1 has been implicated in attenuating LPS- or peptidoglycan-induced biosynthesis proinflammatory cytokines, but role noncoding RNA expression still poorly understood. In this study, we show a direct target microRNA-101 (miR-101). Transfection miR-101 attenuates induction by LPS as well prolonged activation p38 and JNK/stress-activated protein kinase, whereas...

Histone demethylases have emerged as important players in developmental processes. Jumonji domain containing-3 (Jmjd3) has been identified a key histone demethylase that plays critical role the regulation of gene expression; however, vivo function Jmjd3 embryonic development remains largely unknown. To this end, we generated global and conditional knockout mice. Global deletion induces perinatal lethality associated with defective lung development. Tissue stage-specific revealed is...

Background. Creating a clinically acceptable plan in the time-sensitive clinic workflow of brachytherapy is challenging. Deep learning-based dose prediction techniques have been reported as promising solutions with high efficiency and accuracy. However, current studies mainly target EBRT which are inappropriate for brachytherapy, model designed specifically has not yet well-established.Purpose. To predict distribution using novel Squeeze Excitation Attention Net (SE_AN) model.Method. We...

Abstract Efficient detection of surface defects is primary for ensuring product quality during manufacturing processes. To enhance the performance deep learning‐based methods in practical applications, authors propose Dense‐YOLO, a fast defect network that combines strengths DenseNet and you only look once version 3 (YOLOv3). The design lightweight backbone with improved densely connected blocks, optimising utilisation shallow features while maintaining high speeds. Additionally, refine...

A two-degree-of-freedom bistable energy harvester with a spring–magnet oscillator designed for ultra-low frequency vibration harvesting is presented in this paper. It combines magnetic plucking upconversion and variable potential function to achieve high-efficiency response while also being suitably installed applications spatial limitations. lumped parameter model of the piezoelectric dipoles applied develop theoretical system. The prototype fabricated, experimental investigations are...

Abstract Low-velocity water flow in ocean and rivers contains abundant energy that can be harnessed. In this paper, from low-velocity is converted to vibration of an underwater tube, which elastically-supported by two springs move along guiding slots. Piezoelectric cantilever beams are mounted inside the tube harvest inertial energy. Unlike other flow-induced converters, converters perpendicular direction, inclined converter structure applied. It found beneficial for exciting offers a...

Melanoma differentiation-associated gene-5 (MDA5) recognizes distinct subsets of viruses including Encephalomyocarditis virus (EMCV) picornavirus family, but the molecular mechanisms underlying specificity viral recognition MDA5 in immune cells remain obscure. DHX29 is an RNA helicase required for translation 5' structured mRNA host and many picornaviruses (such as EMCV). We identify that DXH29 a key co-sensor, plays significant role specific triggering anti-EMCV immunity. have observed...

Fast and accurate outlining of the organs at risk (OARs) high-risk clinical tumor volume (HRCTV) is especially important in high-dose-rate brachytherapy due to highly time-intensive online treatment planning process high dose gradient around HRCTV. This study aims apply a self-configured ensemble method for fast reproducible auto-segmentation OARs HRCTVs gynecological cancer.

We used a trapping laser to stop chromosome movements in Mesostoma and crane-fly spermatocytes inward of spindle poles after cuts across Potorous tridactylus (rat kangaroo) kidney (PtK2) cell half-spindles. spermatocyte kinetochores execute oscillatory away from the pole for 1-2 h, so we could trap multiple times same spermatocyte. The was focused single point using 63× oil immersion objective. Trap powers 15-23 mW caused kinetochore oscillations or decrease. Kinetochore resumed when...

DNA interstrand crosslinks (ICLs) are toxic lesions that block the progression of replication and transcription. CtIP is a conserved repair protein facilitates end resection in double-strand break (DSB) pathway. Here we show plays critical role during initiation ICL processing replicating human cells distinct from its DSB repair. depletion sensitizes to inducing agents significantly impairs accumulation damage response proteins RPA, ATR, FANCD2, γH2AX, phosphorylated ATM at sites laser...

The purpose of this study is to analyze human sperm motility and energetics in media with different viscosities. Multiple experiments were performed collect parameters using customized computer tracking software that measures the curvilinear velocity (VCL) minimum laser power (Pesc) necessary hold an individual optical trap. Pesc was measured by a 1064 nm Nd:YVO4 continuous wave optically traps motile at 450 mW focused trap spot. VCL frame before trapping. In order under viscous conditions...

Relative concentrations of Streptococcus mutans and sanguis are important parameters in the study dental caries, but current methods measuring these time consuming prone to inaccuracies. We investigate use Raman spectroscopy for relative two bacterial species solid mixtures. To our knowledge, this is first has been used analyze mixtures rather than identify a pure colony. Mixtures streptococcal various ratios measured 200 s using home-built microscope. Spectral correlations with content were...

Abstract Four different species of bacteria (streptococcus mutans, s. sanguis, intermedius and oralis) important in the field oral biology were studied using spontaneous Raman scattering spectroscopy. Spectra several bacterial colonies each type acquired a microscope system. After correction for system background fluorescence, spectra grouped blind clustering algorithm. The clusters thus generated found to correspond closely four bacteria, with 5 misclassifications out 80 samples. may be due...

Near-infrared Raman spectroscopy has been used for species identification of pure microbial specimens more than a decade. More recently, this optical method extended to the analysis containing multiple species. In report, we demonstrate rapid, reagent-free quantitative simplified model oral plaque three bacteria species, S. mutans, sanguis, and gordonii, using near-infrared spectroscopy. spectra were acquired from bacterial mixtures in 200 seconds. A prediction was calibrated by partial...

Although the knowledge-based dose-volume histogram (DVH) prediction has been largely researched and applied in External Beam Radiation Therapy, it is still less investigated domain of brachytherapy. The purpose this study to develop a reliable DVH method for high-dose-rate brachytherapy plans.A workflow combining kernel density estimation (KDE), k-nearest neighbor (kNN), principal component analysis (PCA) was proposed. PCA kNN were first employed together select similar patients based on...

A multi-joystick robotic laser microscope system used to control two optical traps (tweezers) and one scissors has been developed for subcellular organelle manipulation. The use of joysticks provided a "user-friendly" method both trapping cutting organelles such as chromosomes in live cells. This innovative design enabled the clean severing chromosome arms using well ability easily hold pull severed arm tweezers.

Polymers play a central role in controlling the crystallization of pharmaceuticals with effects as divergent amorphous form stabilization and acceleration crystallization. Here, using pyrazinamide hydrochlorothiazide model pharmaceuticals, it is demonstrated that same functional group interactions are responsible for these opposing behaviors whether polymer speeds or slows can be controlled by solubility. This concept applied discovery polymers to maintain drug supersaturation solution:...

Mutations in tumors can create a state of increased cellular plasticity that promotes resistance to treatment. Thus, there is an urgent need develop novel strategies for identifying key factors regulate order combat chemotherapy and radiation Here we report prostate epithelial cell reprogramming could be exploited identify required promoting cancer tumorigenesis plasticity. Deletion phosphatase tensin homolog (Pten) transforming growth factor-beta receptor type 2 (Tgfbr2) may increase...